Overcoming Barriers to Protein Production in Plant Cell Cultures

克服植物细胞培养中蛋白质生产的障碍

• 批准号: 0109936
• 负责人: Michael Shuler
• 金额: --
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0109936&HistoricalAwards=false
• 关键词: Overcoming; Barriers; Protein; Production; Plant

The objective of this project is to identify bottlenecks in heterologous protein production from transgenic plant cell suspensions, and to develop genetic strategies to overcome these limitations. Three model proteins will be studied. These include, hepatitis B surface antigen, Norwalk virus capsid protein, and human secreted alkaline phosphatase. These proteins will be studied in two host systems, i. e. tobacco and soybean suspension cultures. The Principal Investigators (PIs) will test the hypothesis that transcribed mRNA may have increased opportunities for translation in the late exponential/stationary phase as compared with the exponential growth phase due to competition with endogenous mRNA for translational machinery. If this is true, then inducible expression during the late exponential growth/stationary phase might lead to higher product levels.

本项目的目的是确定从转基因植物细胞悬浮液中生产异源蛋白的瓶颈，并开发克服这些限制的遗传策略。 将研究三种模型蛋白。 包括B型肝炎表面抗原、诺瓦克病毒衣壳蛋白和人分泌型碱性磷酸酶。 这些蛋白质将在两个宿主系统中进行研究，e.烟草和大豆悬浮培养物。 主要研究者（PI）将检验以下假设：由于与内源性mRNA竞争翻译机制，与指数生长期相比，转录mRNA在指数生长期后期/稳定期的翻译机会可能增加。 如果这是真的，那么在指数生长后期/稳定期的诱导型表达可能导致更高的产物水平。