Taxol Biosynthesis: Identity of Taxol Direct Precursor in Cell Culture
Taxol Biosynthesis: Identity of Taxol Direct Precursor in Cell Culture
批准号:
9632961
负责人:
Michael Shuler
金额:
$5.0万
依托单位:
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
1996
资助国家:
美国
项目状态:
已结题
起止时间:
1996-04-15 至 1998-03-31
中文摘要
9632961舒勒,这项拟议研究的目标是鉴定和研究在使用某些代谢抑制剂进行植物组织培养实验时,原理调查员实验室最近发现的一种未知紫杉烷的生物合成意义。这些实验包括培养产生紫杉醇的红豆杉,紫杉醇是一种重要的抗癌新药。目前普遍认为巴卡菌素III是紫杉醇的直接生物合成前体。然而,PI最近的实验表明,巴卡菌素III可以被选择性地完全抑制,而不会对紫杉醇的产生产生任何显著影响。其他抑制实验表明,积累了一种未知的紫杉烷,它不是巴卡菌素III,但实际上可能是紫杉醇的直接前体。这项建议概述了一种多学科的方法来鉴定这种未知的化合物,并利用红豆杉细胞培养对紫杉醇生产的操纵知识和生物合成化学中的成熟技术来研究其生物合成意义。***
英文摘要
9632961 Shuler The goal of this proposed research is to identify and investigate the biosynthetic significance of an unknown taxane recently discovered in the Principle Investigators laboratory while performing plant tissue culture experiments with certain metabolic inhibitors. These experiments involved the culture of Taxus species which produce Taxol, an important new anti-cancer drug. It is currently generally accepted that baccatin III is the direct biosynthetic precursor of Taxol. However, recent experiments by the PI indicate that baccatin III could be selectively and completely inhibited without any significant effect on Taxol production. Other inhibition experiments have shown the accumulation of a single unknown taxane, which is not baccatin III, but which could,, in fact, be the direct precursor of Taxol. This proposal outlines a multidisciplinary approach to identify this unknown compound and investigate its biosynthetic significance using knowledge of manipulation of Taxol production by Taxus cell cultures, and established techniques in biosynthetic chemistry. ***
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Targeting cancer stem cell self-renewal and proliferation mechanisms using in vitro microscale models
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批准号:1106153
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项目类别:Standard Grant
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资助金额:$50.0万
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财政年份:2011
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负责人:Michael Shuler
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依托单位:
New, GK-12 -Collaborative Curriculum Development: Interdisciplinary Middle and High School Education in Biomedical Engineering through Graduate Student/Teacher Interaction
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资助金额:$291.0万
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财政年份:2009
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负责人:Michael Shuler
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GOAL: Development of a Cell Culture Analog Device to Assess MultiDrug Resistant Suppressors
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批准号:0342985
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资助金额:$0.0万
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财政年份:2003
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负责人:Michael Shuler
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依托单位:
Overcoming Barriers to Protein Production in Plant Cell Cultures
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批准号:0109936
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项目类别:Continuing grant
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资助金额:$0.0万
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财政年份:2001
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负责人:Michael Shuler
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依托单位:
A Minimal Cell Model to Determine Genomic Structure
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批准号:9909133
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项目类别:Continuing grant
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资助金额:$41.04万
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财政年份:2000
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负责人:Michael Shuler
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依托单位:
Collaborative Research: Metabolic Manipulation of Taxus sp. Cell Cultures for Taxane Production
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批准号:9625405
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项目类别:Standard Grant
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资助金额:$0.0万
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财政年份:1996
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负责人:Michael Shuler
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依托单位:
Research Experience for Undergraduates in Chemical Engineering
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批准号:9322049
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项目类别:Continuing grant
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资助金额:$0.0万
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财政年份:1994
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负责人:Michael Shuler
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依托单位:
Collaborative Research: Improving Post-Translational Processing in Baculovirus Expression System
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批准号:9412249
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项目类别:Continuing grant
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资助金额:$0.0万
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财政年份:1994
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负责人:Michael Shuler
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依托单位:
Continuous Production of Alkaloids from Immobilized Catharanthus Roseus
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批准号:9117252
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项目类别:Standard Grant
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资助金额:$0.0万
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财政年份:1992
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负责人:Michael Shuler
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依托单位:
Use of Insect Cell Culture for Production of Proteins and Viral Pesticides
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批准号:9111091
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项目类别:Continuing Grant
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资助金额:$56.41万
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财政年份:1992
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负责人:Michael Shuler
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依托单位:
Biochemical Engineering Laboratory Development
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批准号:9051404
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项目类别:Standard Grant
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资助金额:$0.0万
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财政年份:1991
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负责人:Michael Shuler
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依托单位:
Engineering Research Equipment: Animal Cell Culture Facility
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批准号:9007140
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项目类别:Standard Grant
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资助金额:$3.07万
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财政年份:1990
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负责人:Michael Shuler
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依托单位:
Development of Cell Culture Analog Systems for Use With Pharmokinetic Models
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批准号:9016798
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项目类别:Standard Grant
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资助金额:$4.0万
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财政年份:1990
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负责人:Michael Shuler
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依托单位:
Bioreactor Development for the Induction, Proliferation, andUse of Structurally Differentiated Plant Tissue Culture for Secondary Metabolite Production
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批准号:8801492
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项目类别:Continuing grant
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资助金额:$0.0万
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财政年份:1988
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负责人:Michael Shuler
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依托单位:
Development of a Rational Basis for Scaling Bioreactors for Production of Proteins from Cloned Eukaryotic Genes and Viral Pesticides Using Insect Cell Cultures
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批准号:8807089
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项目类别:Continuing Grant
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资助金额:$75.98万
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财政年份:1988
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负责人:Michael Shuler
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依托单位:
Design of Bioreactors for Insect Cell Cultures for the Production of Viral Pesticides and Foreign Gene Products
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批准号:8700739
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项目类别:Standard Grant
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资助金额:$0.0万
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财政年份:1987
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负责人:Michael Shuler
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依托单位:
Mechanisms and Control of Protein Excretion in E. Coli as Related to Production of Plasmid-Encoded Proteins in Immobilized Cell Reactors
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批准号:8513612
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项目类别:Continuing grant
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资助金额:$0.0万
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财政年份:1986
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负责人:Michael Shuler
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依托单位:
Bioreactors for Use with Plant Cell Tissue Cultures
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批准号:8503183
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项目类别:Standard Grant
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资助金额:$0.0万
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财政年份:1985
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负责人:Michael Shuler
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依托单位:
Entrapped Plant Cell Cultures
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批准号:8114995
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项目类别:Continuing grant
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资助金额:$0.0万
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财政年份:1982
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负责人:Michael Shuler
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依托单位:
Mathematical Models For Product Formation From Microbial Systems
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批准号:7921259
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项目类别:Continuing grant
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资助金额:$21.68万
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财政年份:1980
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负责人:Michael Shuler
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依托单位:
海外基金