Continuous Production of Alkaloids from Immobilized Catharanthus Roseus

虽然从长春花中提取的吲哚生物碱在美国并不是一种重要的产品，但它是开发有效生产潜在更重要化合物(如紫杉醇)的“使能技术”的有用模式。以前的工作表明，将四种治疗策略结合起来可以使阿玛利辛的胞外产量增加50倍。这四种策略是：(1)诱导；(2)生产介质开发；(3)海藻酸钙固定化；(4)用XAD-7树脂原位吸附。它们协同作用，并在间歇反应器中完成。反复批量种植的尝试表明，维持高水平的生产存在困难。这项工作试图回答以下问题：(1)在长期培养过程中，什么因素会导致生产力损失和细胞生理变化；以及(2)使用四种策略相结合的持续高水平生产是可能的吗？

Although the indole alkaloid ajmalicine, obtained from Catharanthus roseus, is not an important product in the U.S., it serves as a useful model for the development of an "enabling technology" for the efficient production of potentially more important compounds, such as taxol. Previous work has shown that combining four treatment strategies can result in a 50-fold increase in the extracellular production of ajmalicine. These four strategies are: (1) elicitation; (2) production medium development; (3) calcium alginate immobilization; and (4) in situ adsorption with XAD-7 resin. They interacted synergistically, and were accomplished in batch reactors. Attempts with repeated batch cultivation revealed difficulties in sustaining a high level of production. This work seeks to answer the following questions: (1) what factors cause the loss of productivity and alterations in cell physiology during prolonged culture; and (2) is continuous high-level production using the combined four strategies possible?