Continuous Production of Alkaloids from Immobilized Catharanthus Roseus
Continuous Production of Alkaloids from Immobilized Catharanthus Roseus
批准号:
9117252
负责人:
Michael Shuler
金额:
$0.0万
依托单位:
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
1992
资助国家:
美国
项目状态:
已结题
起止时间:
1992-03-01 至 1995-02-28
中文摘要
虽然吲哚生物碱ajmalicine,从 长春花,不是一个重要的产品, 美国,它是一个有用的模式, 高效生产的“赋能技术”, 可能更重要的化合物,如紫杉醇。 以前的研究表明,结合四种治疗方法, 战略可以导致50倍的增长, 阿曲霉素的细胞外产生。 这四 策略是:(1)诱导;(2)生产培养基 (3)海藻酸钙固定化;(4) XAD-7树脂原位吸附。 他们互动 协同作用,并在间歇反应器中完成。 重复分批培养的尝试显示 难以维持高水平的生产。 本文试图回答以下问题:(1) 哪些因素导致生产力下降, 在长期培养期间细胞生理学的改变; (2)是使用该方法的连续高水平生产, 四种策略的结合可能吗?
英文摘要
Although the indole alkaloid ajmalicine, obtained from Catharanthus roseus, is not an important product in the U.S., it serves as a useful model for the development of an "enabling technology" for the efficient production of potentially more important compounds, such as taxol. Previous work has shown that combining four treatment strategies can result in a 50-fold increase in the extracellular production of ajmalicine. These four strategies are: (1) elicitation; (2) production medium development; (3) calcium alginate immobilization; and (4) in situ adsorption with XAD-7 resin. They interacted synergistically, and were accomplished in batch reactors. Attempts with repeated batch cultivation revealed difficulties in sustaining a high level of production. This work seeks to answer the following questions: (1) what factors cause the loss of productivity and alterations in cell physiology during prolonged culture; and (2) is continuous high-level production using the combined four strategies possible?
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Targeting cancer stem cell self-renewal and proliferation mechanisms using in vitro microscale models
-
批准号:1106153
-
项目类别:Standard Grant
-
资助金额:$50.0万
-
财政年份:2011
-
负责人:Michael Shuler
-
依托单位:
New, GK-12 -Collaborative Curriculum Development: Interdisciplinary Middle and High School Education in Biomedical Engineering through Graduate Student/Teacher Interaction
-
批准号:0841291
-
项目类别:Continuing Grant
-
资助金额:$291.0万
-
财政年份:2009
-
负责人:Michael Shuler
-
依托单位:
GOAL: Development of a Cell Culture Analog Device to Assess MultiDrug Resistant Suppressors
-
批准号:0342985
-
项目类别:Standard Grant
-
资助金额:$0.0万
-
财政年份:2003
-
负责人:Michael Shuler
-
依托单位:
Overcoming Barriers to Protein Production in Plant Cell Cultures
-
批准号:0109936
-
项目类别:Continuing grant
-
资助金额:$0.0万
-
财政年份:2001
-
负责人:Michael Shuler
-
依托单位:
A Minimal Cell Model to Determine Genomic Structure
-
批准号:9909133
-
项目类别:Continuing grant
-
资助金额:$41.04万
-
财政年份:2000
-
负责人:Michael Shuler
-
依托单位:
Collaborative Research: Metabolic Manipulation of Taxus sp. Cell Cultures for Taxane Production
-
批准号:9625405
-
项目类别:Standard Grant
-
资助金额:$0.0万
-
财政年份:1996
-
负责人:Michael Shuler
-
依托单位:
Taxol Biosynthesis: Identity of Taxol Direct Precursor in Cell Culture
-
批准号:9632961
-
项目类别:Standard Grant
-
资助金额:$5.0万
-
财政年份:1996
-
负责人:Michael Shuler
-
依托单位:
Research Experience for Undergraduates in Chemical Engineering
-
批准号:9322049
-
项目类别:Continuing grant
-
资助金额:$0.0万
-
财政年份:1994
-
负责人:Michael Shuler
-
依托单位:
Collaborative Research: Improving Post-Translational Processing in Baculovirus Expression System
-
批准号:9412249
-
项目类别:Continuing grant
-
资助金额:$0.0万
-
财政年份:1994
-
负责人:Michael Shuler
-
依托单位:
Use of Insect Cell Culture for Production of Proteins and Viral Pesticides
-
批准号:9111091
-
项目类别:Continuing Grant
-
资助金额:$56.41万
-
财政年份:1992
-
负责人:Michael Shuler
-
依托单位:
Biochemical Engineering Laboratory Development
-
批准号:9051404
-
项目类别:Standard Grant
-
资助金额:$0.0万
-
财政年份:1991
-
负责人:Michael Shuler
-
依托单位:
Engineering Research Equipment: Animal Cell Culture Facility
-
批准号:9007140
-
项目类别:Standard Grant
-
资助金额:$3.07万
-
财政年份:1990
-
负责人:Michael Shuler
-
依托单位:
Development of Cell Culture Analog Systems for Use With Pharmokinetic Models
-
批准号:9016798
-
项目类别:Standard Grant
-
资助金额:$4.0万
-
财政年份:1990
-
负责人:Michael Shuler
-
依托单位:
Bioreactor Development for the Induction, Proliferation, andUse of Structurally Differentiated Plant Tissue Culture for Secondary Metabolite Production
-
批准号:8801492
-
项目类别:Continuing grant
-
资助金额:$0.0万
-
财政年份:1988
-
负责人:Michael Shuler
-
依托单位:
Development of a Rational Basis for Scaling Bioreactors for Production of Proteins from Cloned Eukaryotic Genes and Viral Pesticides Using Insect Cell Cultures
-
批准号:8807089
-
项目类别:Continuing Grant
-
资助金额:$75.98万
-
财政年份:1988
-
负责人:Michael Shuler
-
依托单位:
Design of Bioreactors for Insect Cell Cultures for the Production of Viral Pesticides and Foreign Gene Products
-
批准号:8700739
-
项目类别:Standard Grant
-
资助金额:$0.0万
-
财政年份:1987
-
负责人:Michael Shuler
-
依托单位:
Mechanisms and Control of Protein Excretion in E. Coli as Related to Production of Plasmid-Encoded Proteins in Immobilized Cell Reactors
-
批准号:8513612
-
项目类别:Continuing grant
-
资助金额:$0.0万
-
财政年份:1986
-
负责人:Michael Shuler
-
依托单位:
Bioreactors for Use with Plant Cell Tissue Cultures
-
批准号:8503183
-
项目类别:Standard Grant
-
资助金额:$0.0万
-
财政年份:1985
-
负责人:Michael Shuler
-
依托单位:
Entrapped Plant Cell Cultures
-
批准号:8114995
-
项目类别:Continuing grant
-
资助金额:$0.0万
-
财政年份:1982
-
负责人:Michael Shuler
-
依托单位:
Mathematical Models For Product Formation From Microbial Systems
-
批准号:7921259
-
项目类别:Continuing grant
-
资助金额:$21.68万
-
财政年份:1980
-
负责人:Michael Shuler
-
依托单位:
海外基金