Continuous Production of Alkaloids from Immobilized Catharanthus Roseus

虽然吲哚生物碱ajmalicine，从 长春花，不是一个重要的产品， 美国，它是一个有用的模式， 高效生产的“赋能技术”， 可能更重要的化合物，如紫杉醇。 以前的研究表明，结合四种治疗方法， 战略可以导致50倍的增长， 阿曲霉素的细胞外产生。 这四 策略是：（1）诱导;（2）生产培养基 （3）海藻酸钙固定化;（4） XAD-7树脂原位吸附。 他们互动 协同作用，并在间歇反应器中完成。 重复分批培养的尝试显示 难以维持高水平的生产。 本文试图回答以下问题：（1） 哪些因素导致生产力下降， 在长期培养期间细胞生理学的改变; （2）是使用该方法的连续高水平生产， 四种策略的结合可能吗？

Although the indole alkaloid ajmalicine, obtained from Catharanthus roseus, is not an important product in the U.S., it serves as a useful model for the development of an "enabling technology" for the efficient production of potentially more important compounds, such as taxol. Previous work has shown that combining four treatment strategies can result in a 50-fold increase in the extracellular production of ajmalicine. These four strategies are: (1) elicitation; (2) production medium development; (3) calcium alginate immobilization; and (4) in situ adsorption with XAD-7 resin. They interacted synergistically, and were accomplished in batch reactors. Attempts with repeated batch cultivation revealed difficulties in sustaining a high level of production. This work seeks to answer the following questions: (1) what factors cause the loss of productivity and alterations in cell physiology during prolonged culture; and (2) is continuous high-level production using the combined four strategies possible?