High Voltage Electrophoresis in Capillary Tubes

这项研究是在分析和表面化学的一般领域和分离科学的子领域。本研究的重点是毛细管区带电泳的持续发展，用于分析生物样品的复杂混合物。主要研究领域包括：1)几十秒内的快速分离；2)液相色谱和毛细管区带电泳（CZE）的二维分离；3)通过自动化CZE系统获取理化数据/迁移预测。4)超微尺度分析。本研究利用高压电场（电泳）中电荷的迁移，结合毛细管柱的新发展，实现了带电物质的高效分离。这项新技术将进一步发展并应用于复杂生物样品的分析。

This research is in the general area of analytical and surface chemistry and in the subfield of separation science. This research focuses on the continued development of capillary zone electrophoresis for analysis of complex mixtures from biological samples. Several major areas of research will be investigated including 1) fast separations in a time frame of tens of seconds, 2) two-dimensional separations involving a combination of liquid chromatography and capillary zone electrophoresis (CZE), 3) acquisition of physicochemical data / migration prediction via an automated CZE system, and 4) ultra- microscale analysis. This research uses charge species migration in high voltage fields (electrophoresis) in combination with new developments in capillary columns to achieve efficient separation of charged species. This new technology will be further developed and applied to the analysis of complex biological samples.