High Voltage Electrophoresis in Capillary Tubes

毛细管高压电泳

基本信息

项目摘要

This project, supported by the Analytical and Surface Chemistry Program, is directed at the development of new instrumentation and methodologies to enhance the capabilities of capillary electrophoresis (CE). A novel double-beam absorbance geometry is employed whereby two photodiode arrays are used to obtain spatially resolved detection while correcting for background absorbance and source drift. Development of flow counterbalanced CE, based on a hydrostatically driven liquid flow, is directed at enhancing detectability and resolution. A jet flow interface is being investigated as a rapid means of sample introduction. Size exclusion chromatography is used as an additional separation means prior to the presently employed liquid chromatography-CE multidimensional methodology. Finally, the development of methods for using ultra-high voltages (300 kV) is investigated in an attempt to open a new mode of CE operation. Methods of separating the constituents of complex samples are integral to developments in biological and environmental analyses. Professor Jorgenson's laboratory is a leader in the development of new instrumentation for capillary electrophoresis. Capillary electrophoresis is a very important tool in the analysis of biological samples, such as blood and urine, and is finding increased usage in the analysis of environmental specimens as well. Work in this area has led to faster, more sensitive, and more reliable analyses. The work to be done by Professor Jorgenson and his students is likely to result in new approaches and instrumentation for complex chemical analyses.
该项目由分析和表面化学项目支持,旨在开发新的仪器和方法,以提高毛细管电泳(CE)的能力。一种新的双光束吸收几何结构被采用,其中两个光电二极管阵列被用来获得空间分辨检测,同时校正背景吸收和源漂移。基于流体静力学驱动的液体流动,流量平衡CE的发展旨在提高可探测性和分辨率。射流界面是一种快速的样品导入方法。尺寸排除色谱被用作一种额外的分离手段之前,目前采用液相色谱- ce多维方法学。最后,研究了超高压(300千伏)的发展方法,试图开辟一种新的CE运行模式。分离复杂样品成分的方法是生物和环境分析发展中不可或缺的一部分。乔根森教授的实验室在开发毛细管电泳新仪器方面处于领先地位。毛细管电泳是一种非常重要的生物样品分析工具,如血液和尿液,在环境样品分析中也越来越多地使用。在这个领域的工作已经导致了更快、更敏感和更可靠的分析。乔根森教授和他的学生所做的工作很可能会为复杂的化学分析带来新的方法和仪器。

项目成果

期刊论文数量(0)
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会议论文数量(0)
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James Jorgenson其他文献

James Jorgenson的其他文献

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{{ truncateString('James Jorgenson', 18)}}的其他基金

Upgrade of UNC Chemistry X-ray Single Crystal Diffraction Facility
北卡罗来纳大学化学X射线单晶衍射设备升级
  • 批准号:
    0443601
  • 财政年份:
    2005
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Standard Grant
LCT Mass Spectrometer
LCT质谱仪
  • 批准号:
    0342701
  • 财政年份:
    2004
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Standard Grant
High Voltage Electrophoresis in Capillary Tubes
毛细管高压电泳
  • 批准号:
    9215320
  • 财政年份:
    1992
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Continuing Grant
High Voltage Electrophoresis in Capillary Tubes
毛细管高压电泳
  • 批准号:
    8912926
  • 财政年份:
    1989
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Continuing Grant
High Voltage Electrophoresis in Capillary Tubes
毛细管高压电泳
  • 批准号:
    8607899
  • 财政年份:
    1986
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Continuing Grant
High Voltage Electrophoresis in Capillary Tubes (Chemistry)
毛细管高压电泳(化学)
  • 批准号:
    8213771
  • 财政年份:
    1983
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Continuing Grant

相似海外基金

High-resolution mass spectrometer with Liquid Chromatography (LC) and Capillary Electrophoresis (CE)
具有液相色谱 (LC) 和毛细管电泳 (CE) 功能的高分辨率质谱仪
  • 批准号:
    515835726
  • 财政年份:
    2023
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Major Research Instrumentation
Sodium Dodecyl Sulfate Removal Interface to Enable Characterization of Fragment Impurities in Monoclonal Antibodies by Capillary Electrophoresis Sodium Dodecyl Sulfate Coupled to Mass Spectrometry
十二烷基硫酸钠去除接口可通过十二烷基硫酸钠毛细管电泳与质谱联用对单克隆抗体中的片段杂质进行表征
  • 批准号:
    10759354
  • 财政年份:
    2023
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    $ 50.27万
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Planar Capillary Electrophoresis
平面毛细管电泳
  • 批准号:
    2247387
  • 财政年份:
    2023
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Continuing Grant
New Advances in Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry for Accelerating Biomarker Discovery in Metabolomics
多段注射-毛细管电泳-质谱法加速代谢组学生物标志物发现的新进展
  • 批准号:
    RGPIN-2020-06630
  • 财政年份:
    2022
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Discovery Grants Program - Individual
Development of Desorption Techniques for Capillary Electrophoresis Analysis of Environmentally Toxic and Biochemically Active Contaminants on Nanoparticles and Nanomaterials in Water after Preconcentration by Liposome Encapsulation
脂质体封装预浓缩后水中纳米颗粒和纳米材料上环境毒性和生化活性污染物毛细管电泳分析解吸技术的发展
  • 批准号:
    RGPIN-2018-05320
  • 财政年份:
    2022
  • 资助金额:
    $ 50.27万
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    Discovery Grants Program - Individual
New Advances in Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry for Accelerating Biomarker Discovery in Metabolomics
多段注射-毛细管电泳-质谱法加速代谢组学生物标志物发现的新进展
  • 批准号:
    RGPIN-2020-06630
  • 财政年份:
    2021
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Discovery Grants Program - Individual
SBIR Phase II: Next-Generation Capillary Electrophoresis with Mass Spectrometry for Biopharmaceutical and Biomedical Applications
SBIR 第二阶段:用于生物制药和生物医学应用的下一代毛细管电泳和质谱分析
  • 批准号:
    2025299
  • 财政年份:
    2021
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Cooperative Agreement
Development of Desorption Techniques for Capillary Electrophoresis Analysis of Environmentally Toxic and Biochemically Active Contaminants on Nanoparticles and Nanomaterials in Water after Preconcentration by Liposome Encapsulation
脂质体封装预浓缩后水中纳米颗粒和纳米材料上环境毒性和生化活性污染物毛细管电泳分析解吸技术的发展
  • 批准号:
    RGPIN-2018-05320
  • 财政年份:
    2021
  • 资助金额:
    $ 50.27万
  • 项目类别:
    Discovery Grants Program - Individual
Development of methodologies for the analysis of glycans by capillary electrophoresis and mass spectrometry
开发毛细管电泳和质谱分析聚糖的方法
  • 批准号:
    RGPIN-2016-03929
  • 财政年份:
    2021
  • 资助金额:
    $ 50.27万
  • 项目类别:
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Study on the analytical assay system using capillary gel electrophoresis method for quality and functional evaluation of natural sulfated polysaccharides
毛细管凝胶电泳法评价天然硫酸多糖质量和功能的分析测定系统研究
  • 批准号:
    21K05423
  • 财政年份:
    2021
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    $ 50.27万
  • 项目类别:
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