Acquisition of a 600 MHz NMR Spectrometer

我们建议购买一台600 MHz NMR光谱仪。 的 为新仪器提出的研究将改善我们的 测定高分辨率蛋白质和核酸的能力 二维核磁共振实验中的酸结构 并将提高我们阐明蛋白质和核酸 酸络合物 最有效的获取核酸的方法 酸和蛋白质溶液结构， 二维NOE谱的弛豫矩阵分析，以获得许多 基于核间距和标量耦合的二维NMR 实验产生扭转角约束，结合 利用距离几何的计算技术， 力学和受约束的分子动力学将被探索。 反向检测异质结2D NMR实验将使 共振分配和结构洞察。 改进的 这种能力也有助于我们研究配体与 生物聚合物，从而导致更大的机械见解。

We propose the acquisition of a 600 MHz NMR spectrometer. The research proposed for the new instrument will improve our capability for determining high-resolution protein and nucleic acid structures in solution from two-dimensional NMR experiments and will improve our ability to elucidate protein and nucleic acid complexes. The most effective means of deriving nucleic acid and protein solution structure by using the complete relaxation matrix analysis of 2D NOE spectra to obtain numerous internuclear distances and scalar-coupling-based 2D NMR experiments to yield torsion angle constraints, in conjunction with the computational techniques of distance geometry, molecular mechanics and restrained molecular dynamics will be explored. Reverse-detection heteronuclear 2D NMR experiments will enable resonance assignments and structural insights. The improved capabilities should also aid in our studies of ligand binding to biopolymers, thus leading to greater mechanistic insights.