Acquisition of a 600 MHz NMR Console

9317917 Gorenstein需要购买600 MHz NMR控制台作为Varian VXR 600 NMR光谱仪的升级。还要求对Varian VXR 500 MHz分光计和离线工作站的计算机进行Sparcl 0升级。这些光谱仪升级将提高校园的NMR能力，包括现有的普渡大学生物化学磁共振实验室（PUBMRL）和生物分子NMR，结构和设计的NSF生物设施中心。这些中心和化学系的高场光谱仪致力于提供最先进的高分辨率NMR，计算机图形/分子建模和分子动力学计算方法，以确定生物分子的3D溶液结构和动力学。脉冲场梯度和三重共振，三维和四维核磁共振将通过升级成为可能。升级后的控制台和计算机将允许更大的蛋白质，蛋白质药物复合物和修饰的寡核苷酸类似物（反义和核酶）的解决方案结构测定比目前在校园里可行。在许多合作和主要用户项目中，这些生物分子将使用已建立的合成有机方法或通过自动化DNA或寡肽合成仪合成。 或者，重组DNA方法将用于过量生产野生型和突变蛋白。校园内的各种NMR中心将提供基础设施支持，通过多维NMR方法确定生物分子的3D结构。 设计的生物分子的结构和活性将进行比较，以更好地了解这些分子的生物功能的结构基础。

9317917 Gorenstein Support is requested for purchase of a 600 MHz NMR console as an upgrade to our Varian VXR 600 NMR spectrometer. Sparcl0 upgrades of computers for a Varian VXR 500 MHz spectrometer and off-line workstation are also requested. These spectrometer upgrades will enhance the NMR capabilities on campus, including the existing Purdue University Biochemical Magnetic Resonance Laboratory (PUBMRL) and an NSF Biological Facilities Center in Biomolecular NMR, Structure and Design. These Centers and the high field spectrometers in the Chemistry Department have sought to provide state-of-the-art high resolution NMR, computer graphics/molecular modeling, and molecular dynamics calculation methodologies to determine the 3D solution structure and dynamics of biomolecules. Pulsed field gradient and triple resonance, 3D and 4D NMR will be made possible by the upgrade. The upgraded console and computers will allow the solution structural determination of much larger proteins, protein drug complexes, and modified oligonucleotide analogs (antisense and ribozymes) than are currently feasible on campus. In a number of collaborative and major user projects, these biomolecules will be synthesized using established synthetic organic methods or through automated DNA or oligopeptide synthesizers. Alternatively, recombinant DNA methodologies will be used to overproduce wild-type and mutant proteins. The various NMR Centers on campus will provide the infrastructure support to determine the 3D structure of the biomolecules via multidimensional NMR methods. Structure and activity of the designed biomolecules will be compared to understand better the structural basis for biological function of these molecules.