Acquisition of Beckman XL-1 Analytical Ultracentrifuges

所要求的XL-L分析超速离心机将用于包括研究和教育在内的五项不同但相互关联的活动。(1)该中心将提供一个显著增强的资源，供生物化学系和其他机构教员用于进行NSF/NIH支持的研究。(2)中心将与机构教员密切合作，为继续或新的赠款提案生成初步数据。(3)该中心将与圣安东尼奥的德克萨斯大学健康科学中心以外的研究人员开展合作，使他们能够进行关键的分析性超速离心实验，否则由于缺乏可用的仪器而无法进行这些实验。(4)中心将根据van Holde和Weischet的方法为XL-I开发公共领域软件，允许确定扩散校正的沉积系数分布。这种分析方法使人们能够严格表征其溶液状态行为过于复杂的超分子组件，而不能通过凝胶、过滤、凝胶电泳或密度梯度超速离心法等标准方法进行表征。(5)该中心将在机构和国家层面大力推广分析超速离心学教育。

The requested XL-L analytical ultracentrifuge will be utilized for five distinct but interrelated activities encompassing both research and education. (1) The Center will provide a significantly enhanced resource to be used by both Department of Biochemistry and other institutional faculty to conduct NSF/NIH supported research. (2) The Center will work closely with institutional faculty to generate preliminary data for continuing or new grant proposals. (3) The Center will develop collaborations with researchers outside the University of Texas Health Science Center at San Antonio to allow them to perform key analytical ultracentrifugation experiments that otherwise would not be possible due to the lack of available instrumentation. (4) The Center will develop public domain software for the XL-I based on the method of van Holde and Weischet that permits determination of diffusioncorrected sedimentation coefficient distributions. This analysis method allows one to rigorously characterize supramolecular assemblies whose solution-state behavior is too vomplex to allow characterization by standard methods such as gel; filtration, gel electrophoresis or density gradient ultracentrifugation. (5) The Center will vigorously promote analytical ultracentrifugation education at both the institutional and national levels.