SBIR PHASE II: Genetic Improvement of Penaeus Monodon

SBIR 第二阶段：斑节对虾的遗传改良

• 批准号: 9732533
• 负责人: Robert Shleser
• 金额: $ 39.96万
• 依托单位: Aquatic Farms
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9732533&HistoricalAwards=false
• 关键词: SBIR; PHASE; II; Genetic; Improvement

*** 9732533 Shleser This Small Business Innovation Research (SBIR) Phase II project focuses on genetic improvement of the Black Tiger shrimp, Penaeus monodon. This species represents 75% of world production of farmed shrimp valued at more than 3 billion dollars annually. The overall objective is selective breeding of this shrimp for performance in a farming environment. Specific pathogen free stocks will be genetically identified for selective breeding. The ability to identify members of full-sibling families to establish and maintain pedigree records over several generations is essential to a selective breeding program. Polymerase Chain Reaction (PCR) amplification of introns in conserved nuclear genes will be used to identify alleles that can be used to manage the breeding program. Phase II will identify specific pathogen free stocks; identify alleles that can be used to direct the selection process; develop cost effective methods to monitor populations; and develop additional markers to monitor heterogeneity and reduce inbreeding. Commercial application is expected in genetically improved broodstock for export and post larvae producion in Hawaii to shrimp producers worldwide. ***

小型企业创新研究（SBIR）二期项目重点研究黑虎对虾（Penaeus monodon）的遗传改良。该品种占世界养殖虾产量的75%，每年价值超过30亿美元。总体目标是在养殖环境中选择性地培育这种虾的性能。将对特定的无病原体种群进行遗传鉴定，以便进行选择性育种。识别全同胞家庭成员的能力，建立和保持几代人的血统记录，对于选择性育种计划是必不可少的。聚合酶链反应（PCR）扩增的内含子在保守的核基因将用于识别等位基因，可用于管理育种计划。第二阶段将确定特定的无病原体种群；确定可用于指导选择过程的等位基因；制定具有成本效益的方法监测人口；并开发额外的标记来监测异质性和减少近亲繁殖。预计将在夏威夷向世界各地的虾生产商出口和后期仔虾生产的转基因改良亲鱼中进行商业应用。* * *