Biotransformations Near and Above 100 Degrees Celsius

接近或高于 100 摄氏度的生物转化

• 批准号: 0004257
• 负责人: Michael Adams
• 金额: $ 24.79万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0004257&HistoricalAwards=false
• 关键词: Biotransformations; Near; Above; 100; Degrees

The objective of this project is to focus on enzymes of technical importance from two key organisms, Pyrococcus furiosus and Thermotoga maritima. Both of these have been well characterized by the Principal Investigators and are suitable models for this project. Basic and applied objectives are to be pursued. The project is divided into five main areas. Areas one to four focus on answering specific biochemical questions on four enzymes: chitinases, glycosyl transferases, prolidases, and aminoacylases. The fifth area addresses the optimization of the hyperthermophile gene expression in E. coli.

该项目的目标是集中在两个关键生物，激烈火球菌和海栖热袍菌的酶的技术重要性。 这两种模型均已被主要研究者充分表征，是本项目的合适模型。 应追求基本目标和应用目标。 该项目分为五个主要领域。 第一至第四部分集中于回答关于四种酶的特定生物化学问题：几丁质酶、糖基转移酶、脯氨酰二肽酶和氨基酰化酶。 第五个方面是优化超嗜热菌基因在大肠杆菌中的表达。杆菌