Biocatalysis Near and Above 100 degrees Celsius: Physiological, Enzymological and Engineering Studies

9632657凯利这项建议正在进行研究，以研究两种模型嗜热菌的酶的基本和应用问题，这两种模型是古热球菌(Tmax 105℃)和细菌Thermotoga maritima(Tmax 90 C)。这将使用两种生物整个基因组的序列信息来完成，这些信息将在项目过程中可用。此外，还将审查深海和大陆地热地点中极端嗜热菌的多样性，并将研究它们所含酶的类型，以了解它们的生理意义、耐热性/热活性和/或生物技术潜力。重点放在与碳水化合物和多肽代谢有关的水解酶和氧化还原酶上。将通过重组和生化技术来研究该酶群的多样性和生化特征，以改变底物专一性的模式。超嗜热酶的热稳定和化学稳定的机理也将被讨论。此外，现有酶和新酶的使用将与工业合作一起在各种生物技术应用中进行评估。***

9632657 Kelly This proposal is on research to examine both basic and applied issues of enzymes from two model hyperthermophiles, the archaeon Pyrococcus furiosus (Tmax 105 C) and the bacterium Thermotoga maritima (Tmax 90 C). This will be done using sequence information on the entire genomes of both organisms which will be available during the course of the project. Also, the diversity of hyperthermophiles in both deep sea and continental geothermal sites will be examined, and the types of enzymes they contain will be studied for their physiological significance, thermmostability/thermoactivity and/or biotechnological potential. Emphasis will be placed on hydrolytic and redox enzymes involved in the metabolism of carbohydrates and peptides. Characterization of the diversity and biochemical features of this enzyme group will be investigated by both recombinant and biochemical techniques to change patterns of substrate specificity. Mechanisms of thermostabilization and chemical stabilization of hyperthermophilic enzymes will also be addressed. Also, the use of both existing and new enzymes will be evaluated in a variety of biotechnological applications in conjunction with an industrial collaboration. ***