SGER: Pulsed Laser Readout of Large Molecular Libraries on an Optical Fiber Support

SGER：光纤支持上大分子文库的脉冲激光读数

• 批准号: 0078895
• 负责人: Peter Geissinger
• 金额: $ 6.5万
• 依托单位: University of Wisconsin-Milwaukee
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-15 至 2001-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0078895&HistoricalAwards=false
• 关键词: SGER; Pulsed; Laser; Readout; Large

This Small Grants for Exploratory Research (SGER) award to Professor Peter Geissinger of the University of Wisconsin-Milwaukee is supported by the Analytical and Surface Chemistry Program in the Chemistry Division. The project targets two specific and important areas of analytical chemistry; development of a sensitive and rapid detection scheme for a sensor array and an identification strategy to determine the active components in a combinatorial library. The research uses a novel approach of segmenting an optical fiber into regions that are used in a parallel synthesis scheme. Reactants, along with a fluorophore are added to each fiber segment. Upon excitation with a laser pulse, the evanescent excitation of the fluorophore is detected. The position of the fluorophore is calculated by the time difference between the pulse and the return signal. The position of each compound is known from the synthetic scheme. This allows the active components of the library to be quickly identified. Combinatorial chemistry libraries are becoming increasingly important in synthesis of new chemicals. Accurate and rapid identification of the active species in the library is essential for the optimal use of the library. This project focuses on developing an alternative technique for preparing and screening libraries. This allows quick and economical identification of species that might be effective in applications such as pharmaceuticals. This SGER award to Professor Peter Geissinger of the University of Wisconsin-Milwaukee is supported by the Analytical and Surface Chemistry Program in the Chemistry Division. The project targets two specific and important areas of analytical chemistry; development of a sensitive and rapid detection scheme for a sensor array and an identification strategy to determine the active components in a combinatorial library. The research uses a novel approach of segmenting an optical fiber into regions that are used in a parallel synthesis scheme. Reactants, along with a fluorophore are added to each fiber segment. Upon excitation with a laser pulse, the evanescent excitation of the fluorophore is detected. The position of the fluorophore is calculated by the time difference between the pulse and the return signal. The position of each compound is known from the synthetic scheme. This allows the active components of the library to be quickly identified. Combinatorial chemistry libraries are becoming increasingly important in synthesis of new chemicals. Accurate and rapid identification of the active species in the library is essential for the optimal use of the library. This project focuses on developing an alternative technique for preparing and screening libraries. This allows quick and economical identification of species that might be effective in applications such as pharmaceuticals.

这个探索性研究（SGER）的小赠款奖教授的威斯康星大学密尔沃基分校的彼得盖辛格是由化学部的分析和表面化学计划的支持。该项目针对分析化学的两个特定且重要的领域;开发传感器阵列的灵敏且快速的检测方案以及确定组合库中活性成分的识别策略。该研究使用了一种新的方法，将光纤分割成并行合成方案中使用的区域。将反应物沿着荧光团加入到每个纤维段中。在用激光脉冲激发时，检测荧光团的倏逝激发。通过脉冲和返回信号之间的时间差来计算荧光团的位置。每种化合物的位置从合成方案中已知。这允许快速识别库的活动组件。 组合化学库在新化学品合成中变得越来越重要。 准确和快速地鉴定库中的活性物质对于库的最佳利用是必不可少的。该项目的重点是开发一种制备和筛选文库的替代技术。这允许快速和经济地鉴定可能在诸如药物的应用中有效的物种。 该SGER奖授予威斯康星大学密尔沃基分校的Peter Geissinger教授，由化学系的分析和表面化学项目支持。该项目针对分析化学的两个特定且重要的领域;开发传感器阵列的灵敏且快速的检测方案以及确定组合库中活性成分的识别策略。该研究使用了一种新的方法，将光纤分割成并行合成方案中使用的区域。将反应物沿着荧光团加入到每个纤维段中。在用激光脉冲激发时，检测荧光团的倏逝激发。通过脉冲和返回信号之间的时间差来计算荧光团的位置。每种化合物的位置从合成方案中已知。这允许快速识别库的活动组件。 组合化学库在新化学品合成中变得越来越重要。 准确和快速地鉴定库中的活性物质对于库的最佳利用是必不可少的。该项目的重点是开发一种制备和筛选文库的替代技术。这允许快速和经济地鉴定可能在诸如药物的应用中有效的物种。