Integration of Liquid-Chromatography Mass Spectrometry Across the Chemistry Curriculum

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Chemistry (12) Analytical methods incorporating liquid chromatography (LC) and mass spectrometry are an integral part of research in academic, pharmaceutical, and industrial research laboratories. While LC/MS is discussed in undergraduate analytical lecture courses associated with Instrumental and Quantitative analysis, undergraduates rarely obtain any "hands-on" experience with LC/MS instrumentation. We are integrating into our undergraduate curriculum three laboratory experiments, adapted from the research literature, that utilize electrospray ionization (ESI) and LC/ Ion-Trap MS as an analytical component. Chemistry majors are introduced to LC/MS in their second semester of organic chemistry where they study the reactivity of various amino acids in solid phase peptide synthesis. Undergraduate biochemistry students use ESI and LC/Ion Trap MS to measure the molecular weight of the protein glyceraldehyde-3-phosphate dehydrogenase and the masses of its tryptic fragments. Students in our instrumental analysis laboratory compare ESI and Ion Trap MS to ICP-quadrupole MS and other analytical methods for the determination of lead in the local environment. In addition, undergraduate students are using ESI and LC/ Ion-Trap MS in their research projects.