Integration of Liquid-Chromatography Mass Spectrometry Across the Chemistry Curriculum

液相色谱质谱法在化学课程中的整合

• 批准号: 0088566
• 负责人: Michael Chiarelli
• 金额: $ 7.05万
• 依托单位: Loyola University of Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0088566&HistoricalAwards=false
• 关键词: Integration; Liquid; Chromatography; Mass; Spectrometry

Chemistry (12) Analytical methods incorporating liquid chromatography (LC) and mass spectrometry are an integral part of research in academic, pharmaceutical, and industrial research laboratories. While LC/MS is discussed in undergraduate analytical lecture courses associated with Instrumental and Quantitative analysis, undergraduates rarely obtain any "hands-on" experience with LC/MS instrumentation. We are integrating into our undergraduate curriculum three laboratory experiments, adapted from the research literature, that utilize electrospray ionization (ESI) and LC/ Ion-Trap MS as an analytical component. Chemistry majors are introduced to LC/MS in their second semester of organic chemistry where they study the reactivity of various amino acids in solid phase peptide synthesis. Undergraduate biochemistry students use ESI and LC/Ion Trap MS to measure the molecular weight of the protein glyceraldehyde-3-phosphate dehydrogenase and the masses of its tryptic fragments. Students in our instrumental analysis laboratory compare ESI and Ion Trap MS to ICP-quadrupole MS and other analytical methods for the determination of lead in the local environment. In addition, undergraduate students are using ESI and LC/ Ion-Trap MS in their research projects.

化学(12)结合了液相色谱(LC)和质谱学的分析方法是学术、制药和工业研究实验室研究的一个组成部分。虽然LC/MS在与仪器和定量分析相关的本科生分析课程中讨论，但本科生很少获得任何LC/MS仪器的实践经验。我们正在将三个实验室实验整合到我们的本科课程中，这些实验改编自研究文献，利用电喷雾电离(ESI)和LC/离子陷阱MS作为分析组件。化学专业的学生在有机化学的第二学期学习LC/MS，在那里他们研究各种氨基酸在固相肽合成中的反应性。生物化学专业本科生使用电喷雾化技术和LC/Ion Trap MS来测量蛋白质甘油醛-3-磷酸脱氢酶的相对分子质量及其胰酶片段的质量。我们仪器分析实验室的学生将ESI和离子陷阱MS与电感耦合等离子体四极杆MS和其他分析方法进行比较，以测定当地环境中的铅。此外，本科生在他们的研究项目中使用ESI和LC/离子陷阱MS。