Ethylene and Pathogen Infection Responses in Tomato and Arabidopsis

番茄和拟南芥的乙烯和病原体感染反应

• 批准号: 0091064
• 负责人: Harry Klee
• 金额: --
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2004-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0091064&HistoricalAwards=false
• 关键词: Ethylene; Pathogen; Infection; Responses; Tomato

The interactions of plants and disease-causing pathogens generally fall into two types: compatible and incompatible. Incompatibility leads to resistance while compatibility leads to disease. In the absence of a resistant response, disease symptoms can spread, leading to severe damage or death of the plant. Analysis of mutants unable to synthesize ethylene, salicylic acid (SA) or jasmonic acid (JA), defined a multistage sequence of disease symptom development during a compatible tomato:pathogen interaction. Synthesis of all three phytohormones is essential for complete symptom development. The spread of disease is associated with an ethylene-dependent accumulation of SA. The position of JA within this cascade of events has yet to be determined. We intend to elucidate how these three hormones control symptom development. Experiments will define at a molecular genetic level the roles of ethylene, SA and JA in symptom development in tomato. Through a combination of hormone quantitation and add-backs, the order of action of the three hormones relative to one another will be determined. Microarray analyses will be performed to identify components of the progression from infection to cell death. These experiments will be complemented by work involving Arabidopsis:Xanthomonas interactions. In Arabidopsis, there is differential attenuation of disease symptoms with ethylene insensitive mutants in the various hormone receptors. This result suggests distinct functions for the five ethylene receptors. Molecular and genetic approaches will be used to define the differential responses. These experiments have the potential to identify unique functions for what has heretofore been considered a redundant receptor family.

植物与致病病原体的相互作用一般分为两种类型：相容和不相容。不相容导致抵抗，而相容导致疾病。在缺乏抗性反应的情况下，病害症状会蔓延，导致植物严重受损或死亡。对不能合成乙烯、水杨酸（SA）或茉莉酸（JA）的突变体的分析，确定了相容番茄：病原体相互作用期间疾病症状发展的多阶段序列。所有这三种植物激素的合成是必要的完整的症状发展。疾病的传播与SA的乙烯依赖性积累有关。JA在这一连串事件中的位置还有待确定。我们打算阐明这三种激素是如何控制症状发展的。实验将在分子遗传学水平上确定乙烯、SA和JA在番茄症状发展中的作用。通过激素定量和加回相结合，将确定三种激素相互之间的作用顺序。将进行微阵列分析，以确定从感染到细胞死亡进程的组成部分。这些实验将由拟南芥与黄单胞菌相互作用的工作来补充。在拟南芥中，各种激素受体中乙烯不敏感突变体的疾病症状有不同的衰减。这一结果表明五种乙烯受体具有不同的功能。分子和遗传方法将被用来定义不同的反应。这些实验有可能确定迄今为止被认为是冗余受体家族的独特功能。