Joint NSF/ERA-CAPS: RegulaTomE - Regulating Tomato Quality through Expression

NSF/ERA-CAPS 联合：RegulaTomE - 通过表达调节番茄质量

• 批准号: 1539831
• 负责人: Harry Klee
• 金额: $ 237.42万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1539831&HistoricalAwards=false
• 关键词: Joint; NSF; ERA; CAPS; RegulaTomE

PI: Harry J. Klee (University of Florida-Gainesville) CoPIs: James J. Giovannoni and Zhangjun Fei (USDA-ARS/ Boyce Thompson Institute for Plant Research)ERA-CAPS Collaborators: Cathie Martin (John Innes Centre, Norwich, United Kingdom), Alisdair Fernie (Max Planck Institute for Molecular Plant Physiology, Golm, Germany), Bjoern Usadel (Aachen University, Aachen, Germany), and Dani Zamir (Hebrew University of Jerusalem, Rehovot, Israel) Producers are under pressure to produce more, cheaper food with reduced inputs and impact on the environment. Consumers need and want healthier, better tasting foods than those produced by today's typical production systems focused on quantity rather than quality. This project focuses on tomato, the most economically important fruit/vegetable crop worldwide. The overall goal of this project is to elucidate the genetic mechanisms controlling plant responses to environmental stresses such as drought and to understand how the environment influences the endogenous chemistry of flavor and nutritional qualities in tomato. This research will deliver mechanistic knowledge as well as tangible tools for agronomic and quality improvement of tomato and other crops. Specifically, naturally occurring versions of genes permitting more robust responses to abiotic stress and positively impacting flavor and nutritional quality will be identified and disseminated to public and private breeders so as to inform their selection processes. The effectiveness of these resources and the significance of the knowledge acquired will ensure that the project contributes directly to food security and sustainable fruit cultivation through use of currently untapped genetic information residing in underutilized wild tomato varieties. With regard to outreach and training, the project will provide unique cross-training opportunities for undergraduate and graduate students as well as postdoctoral scientists on both sides of the Atlantic. In addition, the project will provide summer research internships in genomics and bioinformatics for undergraduate students from Fort Valley State, California State University, Fresno and California State University, Sacramento, all minority serving institutions. The twin objectives of this project are to determine the importance of transcriptional regulation of the metabolic pathways defining quality traits in tomato and to identify such transcriptional regulators at the molecular level. The selected quality traits include those determining antioxidant capacity that impacts shelf life and nutritional value as well as those determining fruit flavor. Loci contributing to abiotic stress tolerance will also be identified with the goal of developing more nutritious and sustainable crops. Natural variation available in introgression lines (ILs) resulting from wild species crosses to tomato will be used to assess the importance of transcriptional regulation, identify regulatory genes and assess epigenetic variation. To accomplish these ends, resources that include a genome reference sequence for S. lycopersicoides and metabolite, DNA methylation and transcriptome profiles of IL fruit will be developed and made freely available. Deliverables include regulatory gene identification and new tools for metabolic engineering of fruit quality. This collaborative US-EU project will allow development of tools and resources on a scale unavailable at a national level. All data produced will be freely and continuously shared within the consortium. Specifically, sequence based datasets will be accessible through a consortium database as well as through publicly available data repositories including GenBank, European Bioinformatics Institute (EBI), and the SOL Genomics Network (SGN).

首席研究员：Harry J. Klee（佛罗里达大学盖恩斯维尔分校） 首席研究员：James J. Giovannoni 和 Zhuangjun Fei（USDA-ARS/Boyce Thompson 植物研究所） ERA-CAPS 合作者：Cathie Martin（英国诺里奇约翰英尼斯中心）、Alisdair Fernie（德国戈尔姆马克斯普朗克分子植物生理学研究所）、Bjoern Usadel（亚琛大学，德国亚琛）和 Dani Zamir（耶路撒冷希伯来大学，以色列雷霍沃特）生产者面临着生产更多、更便宜的食品、减少投入和对环境影响的压力。消费者需要并且想要比当今注重数量而不是质量的典型生产系统生产的食品更健康、味道更好。该项目重点关注番茄，这是全球经济上最重要的水果/蔬菜作物。该项目的总体目标是阐明控制植物对干旱等环境胁迫反应的遗传机制，并了解环境如何影响番茄风味和营养品质的内源化学。这项研究将为番茄和其他作物的农艺和质量改进提供机械知识和有形工具。具体来说，将确定天然存在的基因版本，这些基因可以对非生物胁迫做出更强有力的反应，并对风味和营养质量产生积极影响，并将其传播给公共和私人育种者，以便为他们的选择过程提供信息。这些资源的有效性和所获得知识的重要性将确保该项目通过利用未充分利用的野生番茄品种中目前未开发的遗传信息，直接为粮食安全和可持续水果种植做出贡献。在推广和培训方面，该项目将为大西洋两岸的本科生、研究生以及博士后科学家提供独特的交叉培训机会。此外，该项目还将为来自福特谷州立大学、加州州立大学弗雷斯诺分校和加州州立大学萨克拉门托分校以及所有少数族裔服务机构的本科生提供基因组学和生物信息学方面的暑期研究实习机会。 该项目的双重目标是确定定义番茄品质性状的代谢途径转录调控的重要性，并在分子水平上识别此类转录调控因子。选定的质量性状包括决定影响保质期和营养价值的抗氧化能力的质量性状以及决定水果风味的质量性状。还将确定有助于非生物胁迫耐受性的基因座，以开发更有营养和可持续的作物。野生物种与番茄杂交产生的基因渗入系（IL）中存在的自然变异将用于评估转录调控的重要性，识别调控基因并评估表观遗传变异。为了实现这些目标，将开发并免费提供资源，包括番茄番茄基因组参考序列和 IL 果实的代谢物、DNA 甲基化和转录组图谱。可交付成果包括调控基因鉴定和水果质量代谢工程的新工具。这一美国-欧盟合作项目将允许开发国家层面无法实现的大规模工具和资源。所有产生的数据将在联盟内自由、持续地共享。具体来说，基于序列的数据集将可通过联盟数据库以及公共数据存储库（包括 GenBank、欧洲生物信息学研究所 (EBI) 和 SOL 基因组网络 (SGN)）访问。