CAREER: Solid-State NMR of Half-Integer Quadrupolar Vanadium Sites in Vanadium Haloperoxidases

职业生涯：钒卤过氧化物酶中半整数四极钒位点的固态核磁共振

• 批准号: 0350385
• 负责人: Tatyana Polenova
• 金额: $ 50.09万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0350385&HistoricalAwards=false
• 关键词: CAREER; Solid; State; NMR; Half

In this CAREER project funded by the Experimental Physical Chemistry Program of the Chemistry Division, Polenova will study vanadium-containing enzymes and associated model compounds using a variety of NMR techniques. The NMR experiments will be interpreted using density matrix simulations of spectra, and the electric quadrupole gradients for the vanadium will be interpreted using parameterized electronic structure calculations. These methods will be applied to haloperoxidases as well as to studies of vanadyl model compounds with known crystal structure.Metal ions serve a number of purposes in proteins, including catalytic, storage, and structural functions. Many of the mechanistic aspects behind these functions are not well understood. The application of solid-state NMR to metal sites in proteins is an important application. In this work Polenova is able to gather data about metal complexes with high sensitivity and high resolution. These data should have an impact on understanding biologically relevant systems as well as helping advance the state of knowledge at the interface between spectroscopy, bioinorganic chemistry, and metallobiochemistry. The results have the potential to help develop more environmentally friendly methods of catalysis. In her research program Polenova involves not only graduate and undergraduate students, but she also performs outreach to high school students. In addition, her education activities encourage underrepresented groups to pursue careers in science.

在这个由化学部实验物理化学计划资助的职业项目中，Polenova将使用各种核磁共振技术研究含钒酶和相关的模型化合物。核磁共振实验将用光谱的密度矩阵模拟来解释，钒的电四极梯度将用参数电子结构计算来解释。这些方法将被应用于卤代过氧化物酶以及具有已知晶体结构的钒模型化合物的研究。金属离子在蛋白质中具有许多用途，包括催化、存储和结构功能。这些功能背后的许多机械方面并没有得到很好的理解。固体核磁共振在蛋白质金属中心的应用是一个重要的应用。在这项工作中，Polenova能够以高灵敏度和高分辨率收集关于金属络合物的数据。这些数据应该对理解与生物相关的系统产生影响，并有助于推进光谱学、生物无机化学和矿物生物化学之间的界面知识状态。这一结果有可能有助于开发更环保的催化方法。在她的研究项目中，Polenova不仅涉及研究生和本科生，她还向高中生进行推广。此外，她的教育活动鼓励代表不足的群体追求科学事业。