Acquisition of a 600-MHz NMR Spectrometer for Biomolecular and Materials Research

该奖项为购买600 MHz核磁共振(核磁共振)光谱仪提供部分支持，用于多学科研究项目，包括：(1)膜蛋白质结构和动力学，(2)聚合物和催化材料研究，(3)细胞信号分子机制，(4)植物代谢物水平和代谢通量，(5)化学反应动力学。该仪器将提供增强的灵敏度和分辨率能力，以及爱荷华州立大学研究人员目前无法获得的先进核磁共振方法。600 MHz核磁共振的灵敏度和分辨率的提高将使研究膜蛋白构象变化和超分子结构的新的2D和3D固态核磁共振相关技术的实施和开发成为关键。该仪器将极大地推动对现代催化剂和复杂聚合物结构-性质关系的尖端研究，并将促进多维异核溶液核磁共振实验，以询问参与生物信号传输的蛋白质。此外，核磁共振将为植物代谢工程的代谢通量图的构建注入活力。600 MHz核磁共振将使ISU的大量用户受益，他们从事从可生物降解聚合物到酶动力学、从植物碳水化合物到自组装金属络合物的各种研究领域。来自邻近较小机构的学生和教职员工将参与高场乐器的研究和培训，以及本科生研究经验(REU)和少数族裔工程学徒计划(MEAP)的学生。

This award provides partial support for the acquisition of a 600 MHz nuclear magnetic resonance (NMR) spectrometer to be used for multidisciplinary research projects, including: (1) membrane protein structure and dynamics, (2) polymeric and catalytic materials studies, (3) molecular mechanism of cell signaling, (4) plant metabolite levels and metabolic fluxes, and (5) chemical reaction kinetics. The instrument will provide enhanced sensitivity and resolution capabilities, and advanced NMR methods not currently available to Iowa State University researchers. The increased sensitivity and resolution of the 600 MHz NMR will critically enable the implementation and development of novel 2D and 3D solid-state NMR correlation techniques for studying membrane protein conformational changes and supramolecular structures. The instrument will dramatically advance cutting-edge research into the structure-property relations of modern catalysts and complex polymers, and will facilitate multi-dimensional heteronuclear solution NMR experiments that interrogate proteins involved in biological signal transmission. Additionally, the NMR will vitalize the construction of metabolic flux maps for plant metabolic engineering. The 600 MHz NMR will benefit a large user base at ISU, engaged in diverse research areas ranging from biodegradable polymers to enzyme kinetics, from plant carbohydrates to self-assembled metal complexes. Students and faculty from smaller neighboring institutions will participate in research and training on the high-field instrument, as well as students from the Research Experiences for Undergraduates (REU) and Minority Engineering Apprenticeship (MEAP) programs.