Kartenbasierte und Funktionsorientierte genomische Ansätze zur Analyse der Vererbung und Ausprägung von Merkmalen des Wasserbindungsvermögens in kommerziellen Schweinepopulationen

在这个项目中，我们将研究商品猪群体中导致滴水损失变异的基因。一方面，我们将继续分析在水滴计划研究单位的初始阶段确定的候选基因。另一方面，我们还将采用新的尖端实验策略，这些策略仅在过去两年才开始使用。我们计划利用平铺微阵列研究包括非编码RNA在内的所有转录本在QTL间隔的选定区域中的表达。通过这种方法，我们将寻找表型分化猪和QTL基因型分化猪之间表达模式的差异。此外，我们还将进行全基因组关联分析，以直接确定商品猪种群滴漏损失的致病基因。选定的候选基因将被详细描述，以提供标记，以改进与肉类质量有关的育种计划，理想情况下，还将识别致病突变本身。

In this project we will investigate the genes underlying drip loss variation in commercial pig populations. On the one hand, we will continue to analyse candidate genes identified during the initial phase of the DRIP research unit. On the other hand, we will also employ novel cutting edge experimental strategies, which became only available during the last two years. We plan to study the expression of all transcripts including non-coding RNAs in selected regions of the QTL-intervals using tiling microarrays. With this approach we will look for differences in the expression patterns between phenotypically divergent and QTL-genotype divergent pigs. Furthermore, we will perform a genome-wide association analysis to directly identify the causative loci for drip loss in commercial pig populations. Selected candidate genes will be characterised in detail to provide markers for improved breeding programs with respect to meat quality and ideally also to identify the causative mutations themselves.