NER: Surface-enhanced hyper-Raman scattering for biosensing and bioimaging

NER：用于生物传感和生物成像的表面增强超拉曼散射

• 批准号: 0507960
• 负责人: Anne Kelley
• 金额: $ 12.03万
• 依托单位: University of California - Merced
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-15 至 2007-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0507960&HistoricalAwards=false
• 关键词: NER; Surface; enhanced; hyper; Raman

0507960KelleyThis proposal was received in response to Nanoscale Science and Engineering initiative, NSF 04-043, category NER.The nonlinear technique of surface-enhanced hyper-Raman spectroscopy (SEHRS) will be explored for its possible applications to bioanalytical sensing and imaging. The linear analog, surface-enhanced Raman spectroscopy (SERS) is a well-known technique widely applied for obtaining vibrational spectra of analytes at low concentrations, in some cases down to the single-molecule level. SERS is proving valuable for biological sensing applications including intracellular detection, but its limitations include the usual sensitivity of Raman-based methods to interference from fluorescence and sample photodegradation. SEHRS, in which red or nearinfrared laser excitation produces Raman scattering in the near-uv to visible, has a number of potential advantages including less sensitivity to fluorescence, less sample photodamage, greater selectivity for the analyte of interest, better spatial resolution in imaging applications, and the ability to use a simpler detection system because of the large difference between the excitation and detection frequencies. The main disadvantage is the usual weakness of the effect. The goal of this research is to determine the extent to which this can be mitigated by combining the small focal volume of a Raman microscope with metallic nanoparticle surface enhancement of the incident and/or scattered electromagnetic fields from intrinsically hyper-Raman-active chromophores.

0507960 Kelley本提案是响应纳米科学与工程倡议，NSF 04-043，类别NER。将探索表面增强超拉曼光谱（SEHRS）的非线性技术在生物分析传感和成像中的可能应用。线性模拟、表面增强拉曼光谱（Sers）是广泛应用于获得低浓度（在某些情况下低至单分子水平）的分析物的振动光谱的众所周知的技术。Sers被证明对包括细胞内检测在内的生物传感应用是有价值的，但其局限性包括基于拉曼的方法对来自荧光和样品光降解的干扰的通常敏感性。SEHRS，其中红色或近红外激光激发产生近紫外到可见光的拉曼散射，具有许多潜在的优点，包括对荧光的灵敏度较低，样品光损伤较小，对感兴趣的分析物的选择性较大，在成像应用中的空间分辨率较好，以及由于激发和检测频率之间的较大差异而能够使用更简单的检测系统。主要的缺点是通常的效果弱。本研究的目标是确定在何种程度上可以减轻结合小焦体积的拉曼显微镜与金属纳米粒子表面增强的入射和/或散射的电磁场从本质上超拉曼-活性发色团。