SGER: Biologically Compatible Engineered Nanoparticles to Prevent UV-Radiation Induced Damage

SGER：生物相容性工程纳米颗粒可防止紫外线辐射引起的损伤

• 批准号: 0541516
• 负责人: Sudipta Seal
• 金额: --
• 依托单位: The University of Central Florida Board of Trustees
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0541516&HistoricalAwards=false
• 关键词: SGER; Biologically; Compatible; Engineered; Nanoparticles

The goal of the proposed research is focused on determining whether this interaction is specific to ceria nano-particles, and to obtain biochemical evidence in support of this hypothesis to explain the observed effects on human cells. The specific aims are to: 1.) engineering of ultrafine rare earth nanoparticles and subsequent material characterization, 2.) Determine the nature of the interactions of the particles with the human cells by microscopy and 3.) Determine the changes in behavior of the nanoparticles upon exposure to reactive oxygen species. The proposed studies will begin to pinpoint the molecular mechanism by which cerium oxide nanoparticles and similar class of rare earths can protect human cells against UV-induced cell death. The current understanding of interactions between these nanoparticles and human cells is sorely lacking. Little is known about the interaction of these small particles and mammalian systems in terms of toxicity to harness the beneficial aspects of these engineered nanostructures in combating environmental pollution. As interest in nano-scale synthesis of therapeutics increases, a basic understanding of the interactions of metal oxide particles is fundamental to determine potential hazards and risks to exposure to these particles, even in light of the potential benefits demonstrated in the cultured cells.Broader Impact: The use of nano-scale devices promises to be the key to future advances in engineering and medicine. Fundamental knowledge of how human cells interact with nanoparticles thus has very broad impact into the development of nano-scale devices and therapeutics. The training of graduate students will be strongly interdisciplinary - spanning the fields of materials engineering, cell biology, biochemistry. The interdisciplinary nature of the project is possible because of the broad training of the two principal investigators and will facilitate collaboration between these investigators in future research. The proposed research will strengthen the training of graduate students at the University of Central Florida.

拟议研究的目标是确定这种相互作用是否是二氧化铈纳米颗粒所特有的，并获得支持这一假设的生化证据，以解释观察到的对人体细胞的影响。具体目标是：1.) 超细稀土纳米颗粒的工程设计和随后的材料表征，2.) 通过显微镜确定颗粒与人体细胞相互作用的性质，3.) 确定纳米颗粒暴露于活性氧后行为的变化。 拟议的研究将开始查明氧化铈纳米颗粒和类似稀土类可以保护人体细胞免受紫外线诱导的细胞死亡的分子机制。目前对这些纳米颗粒与人体细胞之间相互作用的了解非常缺乏。人们对这些小颗粒和哺乳动物系统在毒性方面的相互作用知之甚少，以利用这些工程纳米结构的有益方面来对抗环境污染。随着人们对纳米级治疗合成的兴趣的增加，对金属氧化物颗粒相互作用的基本了解对于确定暴露于这些颗粒的潜在危害和风险至关重要，即使考虑到培养细胞中所展示的潜在益处也是如此。更广泛的影响：纳米级设备的使用有望成为工程和医学未来进步的关键。因此，关于人体细胞如何与纳米粒子相互作用的基础知识对纳米级设备和治疗方法的开发具有非常广泛的影响。研究生的培养将具有很强的跨学科性——跨越材料工程、细胞生物学、生物化学领域。由于两位主要研究人员接受了广泛的培训，该项目的跨学科性质是可能的，并将促进这些研究人员在未来研究中的合作。拟议的研究将加强中佛罗里达大学研究生的培训。