Biocatalytic Nanolithography

生物催化纳米光刻

• 批准号: 0600513
• 负责人: Robert Clark
• 金额: $ 26万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0600513&HistoricalAwards=false
• 关键词: Biocatalytic; Nanolithography

This grant provides funding to advance high-throughput manufacturing methods for creating surface patterns at the nanoscale with immobilized enzymes through catalytic stamping, designated as biocatalytic nanolithography. There are three essential components to the proposed work. The first component is devoted to the development of the protein chemistry required to enable the manufacturing processes. For this component the density of both enzyme and substrate will be varied to determine the optimum conditions for enzymatic transformation near surfaces. Additionally, the surface activity of several groups of enzymes will be studied to expand the group of enzymes available for pattern transfer. The second component focuses on the development of master stamps, a parametric study of stamping conditions, and the use of instrumentation with appropriate metrology required to automate manipulation of the stamps with nanometer precision. The third component is focused on providing an integrative educational and research experience for both graduate and undergraduate students, leveraging the currently funded NSF IGERT program in biologically inspired materials and material systems. If successful, a new method of manufacturing at the nanoscale will result, utilizing nature's tools (enzymes) for catalyzing reactions on a surface and employing engineering tools required to automate the process. The development of a stamp in lithographic manufacturing processes at the nano- or micro-scale constitutes one of the costly steps. By initially functionalizing the master stamp with molecules that facilitate reversible immobilization chemistry, the masters can be re-inked with new catalyst, reducing engineering time and cost in high-throughput manufacturing. This new catalyst might simply replace inactive (worn-out) enzyme, or an alternative enzyme that catalyzes different chemistry can be substituted. Advantages of the process include 1) lack of physical transfer of molecules from the stamp to the patterned surface, enabling high resolution, 2) inherent selectivity of enzymes that facilitate orthogonal reactivity and the ability to create complex patterns through multiple stamping procedures, and 3) a green chemistry that avoids the use of expensive, toxic, and flammable solvents, heavy metals and other reagents. Students trained in this evolving field of research will contribute to the use of Nature's tools for manufacturing at the nanoscale.

这笔赠款提供资金来推进高通量制造方法，通过催化冲压（称为生物催化纳米光刻）用固定化酶创建纳米级表面图案。 拟议工作由三个基本组成部分组成。 第一个部分致力于开发制造过程所需的蛋白质化学。 对于该组分，酶和底物的密度将发生变化，以确定表面附近酶促转化的最佳条件。 此外，还将研究几组酶的表面活性，以扩大可用于图案转移的酶组。 第二部分重点是母版印模的开发、冲压条件的参数化研究，以及使用具有纳米精度自动操作印模所需的适当计量仪器。 第三部分的重点是利用目前资助的 NSF IGERT 项目在生物启发材料和材料系统方面为研究生和本科生提供综合教育和研究经验。 如果成功，将产生一种新的纳米级制造方法，利用自然界的工具（酶）催化表面反应，并采用自动化该过程所需的工程工具。 在纳米或微米尺度的光刻制造工艺中开发印模是成本高昂的步骤之一。 通过最初用促进可逆固定化化学的分子对母版印章进行功能化，母版可以用新的催化剂重新上墨，从而减少高通量制造中的工程时间和成本。 这种新催化剂可能只是取代无活性（磨损）的酶，或者可以替代催化不同化学物质的替代酶。 该工艺的优点包括：1) 分子从印模到图案表面的物理转移不存在，从而实现了高分辨率；2) 酶固有的选择性，促进了正交反应性，并且能够通过多种冲压程序创建复杂的图案；3) 绿色化学，避免使用昂贵、有毒和易燃的溶剂、重金属和其他试剂。 在这个不断发展的研究领域接受过培训的学生将为使用自然工具进行纳米级制造做出贡献。