A Soft Polymer-on-Silicon Nano Photonic Device for High-Speed Fluorescence Multi-Spectrum Acquisition in Integrated Microfluidic Immunoassay System

本研究的目的是开发一种新型的纳米光子可调谐装置，用于高速单探测器光谱测量。该装置集成在微流体系统中，以简单的光学和较少的计算需求，在流式微球为基础的荧光免疫分析中实现高通量多分析物检测。该方法基于新型聚合物-硅混合微机电系统技术。它允许在三维聚合物微观结构的表面上刻印纳米级特征。该系统的光谱采集速度预计将超过100 nm/ms，并允许在微流体系统中进行实时光谱。智力优势：本研究开发的创新纳米光子技术将指导波长区分检测的未来发展，用于多种化学和生物物种的识别和定量。集成了微流体细胞培养和免疫分析系统，该设备导致了一种不存在的科学仪器的发展，允许在微流体通道中对细胞参数的时间变化进行现场监测。更广泛的影响：由于其成本效益和在生命科学研究和开发中的实用性，开发的微流控氟免疫分析系统可能会找到新的商业市场。拟议的项目将促进培养新一代工程师和科学家的绝佳机会，他们将跨越传统研究领域的界限，创造新的研究途径。

0601237KurabayashiThe objective of this research is to develop a novel nano photonic tunable device for high-speed single-detector spectral measurement. The proposed device is integrated in a microfluidic system to achieve high-throughput multi-analyte detection in flow-through microsphere-based fluoroimmunoassay with simple optics and with less computational requirements. The approach is based on the new polymer-silicon hybrid microelectromechanical systems technology. It allows imprinting of nanoscale features on the surface of the three-dimensional polymer microstructure. The spectrum acquisition speed of the system is expected to exceed 100 nm/ms and to allow real-time spectroscopy in a microfluidic system. Intellectual merit:The innovative nano photonic technology developed in this research will guide future advancements of wavelength-discriminating detection for the identification and quantification of multiple chemical and biological species. Integrated with microfluidic cell culture and immunoassay systems, the device leads to development of a non-existing scientific instrument that permits in-situ monitoring of time variations of cellular parameters in a microfluidic channel. Broader Impact:The developed microfluidic fluoroimmunoassay system may find new commercial markets because of its cost-effectiveness and utility in life sciences research and development. The proposed project will promote an excellent opportunity to train a new generation of engineers and scientists who will cross the boundaries of traditional research fields and create new avenues of research.