Molecular-scale Interactions at the Peptide-mineral Interface

肽-矿物质界面的分子尺度相互作用

• 批准号: 0613972
• 负责人: Pupa Gilbert
• 金额: --
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0613972&HistoricalAwards=false
• 关键词: Molecular; scale; Interactions; Peptide; mineral

ABSTRACT CHE-0613972De Stasio Gilbert/Wisconsin - MadisonBiomineralization is the production of minerals by living organisms. The structure and formation of natural biominerals are controlled at the molecular scale by proteins, but most aspects of key organic-inorganic interactions remain unclear. With support from the Analytical and Surface Chemistry Program in the Division of Chemistry and the Biomaterials Program in the Division of Material Research, Gelsomina "Pupa" De Stasio Gilbert and her group in the University of Wisconsin-Madison Department of Physics are employing and developing spectroscopic methods to study molecular-scale interactions at the interfaces between peptides or proteins on one hand, and minerals or their precursor nanoparticles on the other. This will reveal how mineral structure and protein conformation influence one another, and how they evolve together during biomineralization. In particular, they focus on the interactions between peptides and calcium phosphate and calcium carbonate, which are relevant to the early steps in bone, teeth and shell formation.Both graduate and undergraduate students are involved in the proposed experiments, and are assisting with preparation of biomineralization instructional materials for college and high school students. These materials will integrate the research results acquired by the students themselves, thereby integrating research, teaching and learning. Students will also invent and prepare demonstration stands and hands-on experiences on biomineralization for the broader community at the UW Synchrotron Radiation Center annual Open House, which hosts more than 200 people from the general public once a year, and for the "Microscopy of Life" course, which the PI teaches every spring (40 graduate and undergraduate students from 8 departments). Research in biomineralization is a multidisciplinary field, including chemistry, physics, biology, and materials science. The impact of the proposed experiments, therefore, will reach far beyond the boundaries of chemistry and physics.

de Stasio Gilbert/Wisconsin - madison生物矿化是生物体产生矿物质的过程。天然生物矿物的结构和形成在分子尺度上由蛋白质控制，但主要的有机-无机相互作用的大多数方面仍不清楚。在化学学部的分析与表面化学项目和材料研究部的生物材料项目的支持下，威斯康星大学麦迪逊分校物理系的Gelsomina“Pupa”De Stasio Gilbert和她的团队正在采用并开发光谱方法来研究肽或蛋白质与矿物质或其前体纳米颗粒之间界面的分子尺度相互作用。这将揭示矿物结构和蛋白质构象如何相互影响，以及它们在生物矿化过程中如何共同进化。他们特别关注肽与磷酸钙和碳酸钙之间的相互作用，这与骨骼、牙齿和外壳形成的早期步骤有关。研究生和本科生都参与了拟议的实验，并协助准备大学和高中学生的生物矿化教学材料。这些材料将整合学生自己获得的研究成果，从而集研究、教与学于一体。学生还将在西澳大学同步辐射中心的年度开放日上为更广泛的社区发明和准备示范台，并为生物矿化提供实践经验，该开放日每年接待200多名公众，以及PI每年春季教授的“生命显微镜”课程（来自8个部门的40名研究生和本科生）。生物矿化研究是一个多学科领域，包括化学、物理、生物和材料科学。因此，所提出的实验的影响将远远超出化学和物理的界限。