Molecular-scale Interactions at the Peptide-mineral Interface

肽-矿物质界面的分子尺度相互作用

• 批准号: 0613972
• 负责人: Pupa Gilbert
• 金额: --
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0613972&HistoricalAwards=false
• 关键词: Molecular; scale; Interactions; Peptide; mineral

ABSTRACT CHE-0613972De Stasio Gilbert/Wisconsin - MadisonBiomineralization is the production of minerals by living organisms. The structure and formation of natural biominerals are controlled at the molecular scale by proteins, but most aspects of key organic-inorganic interactions remain unclear. With support from the Analytical and Surface Chemistry Program in the Division of Chemistry and the Biomaterials Program in the Division of Material Research, Gelsomina "Pupa" De Stasio Gilbert and her group in the University of Wisconsin-Madison Department of Physics are employing and developing spectroscopic methods to study molecular-scale interactions at the interfaces between peptides or proteins on one hand, and minerals or their precursor nanoparticles on the other. This will reveal how mineral structure and protein conformation influence one another, and how they evolve together during biomineralization. In particular, they focus on the interactions between peptides and calcium phosphate and calcium carbonate, which are relevant to the early steps in bone, teeth and shell formation.Both graduate and undergraduate students are involved in the proposed experiments, and are assisting with preparation of biomineralization instructional materials for college and high school students. These materials will integrate the research results acquired by the students themselves, thereby integrating research, teaching and learning. Students will also invent and prepare demonstration stands and hands-on experiences on biomineralization for the broader community at the UW Synchrotron Radiation Center annual Open House, which hosts more than 200 people from the general public once a year, and for the "Microscopy of Life" course, which the PI teaches every spring (40 graduate and undergraduate students from 8 departments). Research in biomineralization is a multidisciplinary field, including chemistry, physics, biology, and materials science. The impact of the proposed experiments, therefore, will reach far beyond the boundaries of chemistry and physics.

摘要CHE -0613972DE Stasio Gilbert/Wisconsin -MadisonBiomineration是由活生物体生产的矿物质。 自然生物矿物质的结构和形成是通过蛋白质在分子尺度控制的，但是关键的有机无机相互作用的大多数方面尚不清楚。 在材料研究划分的化学和生物材料分析和生物材料计划中的分析和表面化学计划的支持下，Gelsomina“ Pupa” De Stasio Gilbert及其在威斯康星大学麦迪逊分校物理学系的小组正在采用和开发光谱方法，以研究分子相互作用，以研究分子相互作用或在粉刺之间或蛋白质之间进行分子相互作用，或者另一个纳米颗粒。 这将揭示矿物结构和蛋白质构象如何相互影响，以及它们在生物矿化过程中如何共同发展。 特别是，它们专注于肽与磷酸钙和碳酸钙之间的相互作用，这些相互作用与骨骼，牙齿和壳形成的早期步骤有关。毕业生和本科生都参与了拟议的实验，并正在协助准备大学和高中生的生物矿化教学材料。 这些材料将整合学生本身获得的研究结果，从而整合研究，教学。 学生还将在UW Synchrotron辐射中心年度开放日内发明和准备有关更广泛社区的生物矿化的演示摊和动手经验，该公寓每年一次拥有200多人来自公众的200人，以及“ PI的“生命显微镜”课程”，PI每年春季教授40名研究生（40名研究生毕业生，来自8个部门的学生））。 生物矿化研究是一个多学科领域，包括化学，物理，生物学和材料科学。 因此，提出的实验的影响将超出化学和物理学的边界。