Role and Regulation of Early Cell Polarity in Sea Urchin Embryos

海胆胚胎早期细胞极性的作用和调控

• 批准号: 0615233
• 负责人: David Burgess
• 金额: $ 15万
• 依托单位: Boston College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0615233&HistoricalAwards=false
• 关键词: Role; Regulation; Early; Cell; Polarity

Burgess, DavidIOB-0615233 Role and Regulation of Early Cell Polarity in Sea Urchin EmbryosOne goal of this research is to provide an opportunity for the investigator to re-tool his research background and perspective. He is an active cell biologist who has studied recently the last stages of cell division using sea urchin eggs as a model system. Interestingly, an observation his laboratory made on dividing eggs has opened up an opportunity to address an important question in the area of developmental biology. To gain a better understanding and depth in the field of developmental biology and because research will be conducted at the Marine Biological Laboratory (MBL), the investigator will attend one of the best courses in developmental biology: the Embryology: Concepts and Techniques in Modern Developmental Biology course. The research to be done addresses questions about how and when apical-basolateral (inside out) polarity originates in embryos and how the cell's cytoskeleton is regulated to assist in the polarization. The investigator and students will study the role of precocious cell polarity in cells isolated from early sea urchin embryos and how their distinct surfaces play a role in normal development. Single cell surgery, combined with cellular localization studies of proteins thought to control cell polarity will be performed. This research should result in a greater understanding of the mechanisms of precocious embryonic cell polarity and its role in early embryos. Becoming a successful scientist who happens to be Cherokee and raised in Native culture has provided the investigator opportunities to make sure opportunities exist for the next generation of minority scientists. He will continue mentoring and involving American Indian, African American and Hispanic undergraduates in his research and continue working both nationally and locally in the policy arena to help develop opportunities for underrepresented minorities in science.

Burgess，DavidIOB-0615233海胆胚胎早期细胞极性的作用和调节本研究的一个目标是为研究人员提供一个重新调整其研究背景和观点的机会。 他是一位活跃的细胞生物学家，最近使用海胆卵作为模型系统研究了细胞分裂的最后阶段。 有趣的是，他的实验室对卵子分裂的观察为解决发育生物学领域的一个重要问题提供了机会。为了更好地了解和深入发育生物学领域，并且因为研究将在海洋生物实验室（MBL）进行，研究人员将参加发育生物学中最好的课程之一：胚胎学：现代发育生物学课程的概念和技术。即将进行的研究解决了关于顶侧-底侧（由内而外）极性如何以及何时起源于胚胎以及细胞的细胞骨架如何被调节以帮助极化的问题。研究人员和学生将研究早熟细胞极性在从早期海胆胚胎分离的细胞中的作用，以及它们独特的表面如何在正常发育中发挥作用。将进行单细胞手术，结合被认为控制细胞极性的蛋白质的细胞定位研究。这项研究将使我们更好地理解早熟胚胎细胞极性的机制及其在早期胚胎中的作用。 成为一名成功的科学家谁碰巧是切罗基和提出的土著文化提供了调查机会，以确保机会存在的下一代少数民族科学家。 他将继续指导和参与美国印第安人，非洲裔美国人和西班牙裔本科生在他的研究，并继续在国家和地方的政策竞技场工作，以帮助发展机会在科学代表性不足的少数民族。