Role and Regulation of Early Cell Polarity in Sea Urchin Embryos

海胆胚胎早期细胞极性的作用和调控本研究的目的之一是为研究者提供一个机会，重新整理他的研究背景和观点。他是一位活跃的细胞生物学家，最近用海胆卵作为模型系统研究细胞分裂的最后阶段。有趣的是，他的实验室对卵细胞分裂的观察为解决发育生物学领域的一个重要问题提供了一个机会。为了更好地了解和深入发育生物学领域，并且由于研究将在海洋生物实验室（MBL）进行，研究者将参加发育生物学最好的课程之一：胚胎学：现代发育生物学的概念和技术课程。这项有待完成的研究解决了以下问题：胚胎的顶-底侧（由内到外）极性是如何以及何时产生的，以及细胞骨架是如何调节以协助极化的。研究者和学生将研究从早期海胆胚胎中分离的细胞中早熟细胞极性的作用，以及它们独特的表面如何在正常发育中发挥作用。将进行单细胞手术，结合被认为控制细胞极性的蛋白质的细胞定位研究。这项研究将有助于更好地理解早衰胚胎细胞极性的机制及其在早期胚胎中的作用。成为一名成功的科学家，碰巧是切罗基人，在土著文化中长大，这为研究者提供了机会，确保下一代少数民族科学家有机会。他将继续指导美国印第安人、非洲裔美国人和西班牙裔本科生参与他的研究，并继续在全国和地方的政策领域工作，帮助在科学领域被忽视的少数民族发展机会。

Burgess, DavidIOB-0615233 Role and Regulation of Early Cell Polarity in Sea Urchin EmbryosOne goal of this research is to provide an opportunity for the investigator to re-tool his research background and perspective. He is an active cell biologist who has studied recently the last stages of cell division using sea urchin eggs as a model system. Interestingly, an observation his laboratory made on dividing eggs has opened up an opportunity to address an important question in the area of developmental biology. To gain a better understanding and depth in the field of developmental biology and because research will be conducted at the Marine Biological Laboratory (MBL), the investigator will attend one of the best courses in developmental biology: the Embryology: Concepts and Techniques in Modern Developmental Biology course. The research to be done addresses questions about how and when apical-basolateral (inside out) polarity originates in embryos and how the cell's cytoskeleton is regulated to assist in the polarization. The investigator and students will study the role of precocious cell polarity in cells isolated from early sea urchin embryos and how their distinct surfaces play a role in normal development. Single cell surgery, combined with cellular localization studies of proteins thought to control cell polarity will be performed. This research should result in a greater understanding of the mechanisms of precocious embryonic cell polarity and its role in early embryos. Becoming a successful scientist who happens to be Cherokee and raised in Native culture has provided the investigator opportunities to make sure opportunities exist for the next generation of minority scientists. He will continue mentoring and involving American Indian, African American and Hispanic undergraduates in his research and continue working both nationally and locally in the policy arena to help develop opportunities for underrepresented minorities in science.