Role and Regulation of Early Cell Polarity in Sea Urchin Embryos

Burgess，DavidIOB-0615233在海胆胚胎早期细胞极性中的作用和调控本研究的目的之一是为研究人员提供一个机会来重新调整他的研究背景和观点。他是一位活跃的细胞生物学家，最近使用海胆卵作为模型系统研究了细胞分裂的最后阶段。有趣的是，他的实验室对卵子分裂的观察为解决发育生物学领域的一个重要问题提供了机会。为了更好地了解和深入发育生物学领域，由于研究将在海洋生物实验室(MBL)进行，研究人员将参加最好的发育生物学课程之一：胚胎学：现代发育生物学的概念和技术课程。将要进行的研究涉及胚胎如何以及何时产生心尖-基侧(内向外)极性，以及细胞的细胞骨架如何被调节以帮助极化。研究人员和学生将研究从早期海胆胚胎分离的细胞中早熟细胞极性的作用，以及它们独特的表面如何在正常发育中发挥作用。将进行单细胞手术，结合被认为控制细胞极性的蛋白质的细胞定位研究。这项研究应该能更好地理解早熟胚胎细胞极化的机制及其在早期胚胎中的作用。成为一名碰巧是切诺基人并在土著文化中长大的成功科学家，为研究人员提供了机会，以确保下一代少数族裔科学家有机会。他将继续指导和让美国印第安人、非裔美国人和西班牙裔本科生参与他的研究，并继续在国家和地方政策领域工作，帮助未被充分代表的少数族裔在科学领域发展机会。

Burgess, DavidIOB-0615233 Role and Regulation of Early Cell Polarity in Sea Urchin EmbryosOne goal of this research is to provide an opportunity for the investigator to re-tool his research background and perspective. He is an active cell biologist who has studied recently the last stages of cell division using sea urchin eggs as a model system. Interestingly, an observation his laboratory made on dividing eggs has opened up an opportunity to address an important question in the area of developmental biology. To gain a better understanding and depth in the field of developmental biology and because research will be conducted at the Marine Biological Laboratory (MBL), the investigator will attend one of the best courses in developmental biology: the Embryology: Concepts and Techniques in Modern Developmental Biology course. The research to be done addresses questions about how and when apical-basolateral (inside out) polarity originates in embryos and how the cell's cytoskeleton is regulated to assist in the polarization. The investigator and students will study the role of precocious cell polarity in cells isolated from early sea urchin embryos and how their distinct surfaces play a role in normal development. Single cell surgery, combined with cellular localization studies of proteins thought to control cell polarity will be performed. This research should result in a greater understanding of the mechanisms of precocious embryonic cell polarity and its role in early embryos. Becoming a successful scientist who happens to be Cherokee and raised in Native culture has provided the investigator opportunities to make sure opportunities exist for the next generation of minority scientists. He will continue mentoring and involving American Indian, African American and Hispanic undergraduates in his research and continue working both nationally and locally in the policy arena to help develop opportunities for underrepresented minorities in science.