NIRT: Single molecule detection in living cells using carbon nanotube optical probes

NIRT：使用碳纳米管光学探针进行活细胞中的单分子检测

• 批准号: 0708459
• 负责人: Michael Strano
• 金额: $ 99.93万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-15 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0708459&HistoricalAwards=false
• 关键词: NIRT; Single; molecule; detection; living

0708459Strano This proposal was received in response to the Active Nanostructures and Nanosystems initiative, NSF 306-595, category NIRT. This proposal seeks to demonstrate real time, sustained optical signal transduction from living cells at the single molecule level. Nanotechnology is yielding new classes of materials with unique optical properties that can provide innovative engineering solutions to traditionally difficult biological problems. While single molecule detection techniques have significantly impacted our understanding of biology, limitations such as photobleaching, intermittent emission (blinking), unfavorable wavelengths and auto-fluorescence have restricted in-vitro and in-vivo single molecule studies to superficial regions of biological systems. The high electronic density of states of carbon nanotubes enables their spectroscopic characterization at the single nanotube level. Hence, it is now theoretically possible to transduce single molecule adsorption events at a nanotube surface. Furthermore, carbon nanotubes are unique in that they do not photo-bleach, show no intermittent blinking and are detectable at the single molecule level in the near infrared, where auto-fluorescent background is negligible. In this proposal, the research team will demonstrates the engineering of single walled carbon nanotubes by attaching recognition ligands, which modulate the optical properties in response to a specific molecular adsorption. Preliminary data has been shown for ultra sensitive detection of glucose and streptavidin using prototype carbon nanotube-based optical probes. In addition, the PI demonstrates that wrapping DNA oligonucliotides and other biomolecules around nanotube sensors can deliver them directly into the cytoplasm of murine myoblast stem cells. Thus they present, as proof of concept, the first nanotube spectroscopy from within a living cell.

本提案是对NSF 306-595 NIRT类主动纳米结构和纳米系统倡议的响应。这项提议试图在单分子水平上展示活细胞的实时、持续的光信号转导。纳米技术正在产生具有独特光学性质的新型材料，可以为传统上难以解决的生物学问题提供创新的工程解决方案。虽然单分子检测技术已经显著地影响了我们对生物学的理解，但诸如光漂白、间歇性发射(闪烁)、不良波长和自体荧光等限制了体外和体内单分子研究仅限于生物系统的表面区域。碳纳米管的高电子态密度使得它们能够在单纳米管水平上进行光谱表征。因此，从理论上讲，现在有可能在纳米管表面传递单分子吸附事件。此外，碳纳米管的独特之处在于它们不会光漂白，不会间歇性闪烁，并且可以在近红外的单分子水平上检测到，其中自动荧光背景可以忽略不计。在这项提案中，研究小组将展示通过附加识别配体来设计单壁碳纳米管的工程，识别配体可以响应特定的分子吸附来调节光学性质。初步数据显示，利用原型碳纳米管光学探针可以超灵敏地检测葡萄糖和链霉亲和素。此外，PI表明，将DNA寡核苷酸和其他生物分子包裹在纳米管传感器周围可以将它们直接输送到小鼠成肌细胞干细胞的细胞质中。因此，作为概念的证明，他们提出了第一个来自活细胞内的纳米管光谱。