Protein toxin complexes of Photorhabdus luminescens

发光杆菌蛋白毒素复合物

• 批准号: 183175329
• 负责人: Professor Dr. Klaus Aktories
• 金额: --
• 依托单位: Abteilung I
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/183175329?language=en
• 关键词: Protein; toxin; complexes; Photorhabdus; luminescens

Photorhabdus luminescens is mutualistically associated with nematodes, which invade and kill insect larvae by means of the bacteria. Therefore, nematodes carrying bacteria are used as insecticides. Toxin complexes (Tc toxins) from P. luminescens, consisting of three components TcA, TcB and TcC, are essential for prevailing of bacteria and nematodes in the insect host. Recently, we have shown that two TcC components (TccC3 and TccC5) are ADP-ribosyltransferases. Whereas TccC3 ADP-ribosylates actin at threonine-148 to induce actin polymerization, TccC5 modifies Rho-GTPases at glutamine-63/61 to constitutively activate the GTPases. In the project the toxin complex is analyzed. The functional consequences and structural and biochemical requirements of the ADP-ribosylation will be studied. Furthermore, topic of the project is the analysis of the functions of TcA and TcB components of Tc toxin. It is proposed that the study elucidates the mode of actions and the interactions of the components of a new toxin family, which is found in several entomopathogenic bacteria but also in human pathogens.

发光杆菌与线虫共生，线虫通过发光杆菌侵入并杀死昆虫幼虫。因此，携带细菌的线虫被用作杀虫剂。发光假单胞菌（P. luminescens）的毒素复合物（Tc toxins）由TcA、TcB和TcC三种组分组成，是细菌和线虫在昆虫宿主中繁殖所必需的。最近，我们已经表明，两个TcC组件（TccC 3和TccC 5）是ADP-核糖基转移酶。TccC 3在苏氨酸-148处ADP-核糖基化肌动蛋白以诱导肌动蛋白聚合，而TccC 5在谷氨酰胺-63/61处修饰Rho-GTP酶以组成型激活GTP酶。在该项目中，分析了毒素复合物。ADP-核糖基化的功能后果和结构和生化要求将被研究。此外，本项目的主题是分析Tc毒素的TcA和TcB组分的功能。有人提出，这项研究阐明了一个新的毒素家族，这是在几个昆虫病原细菌，但也在人类病原体的成分的作用模式和相互作用。

项目成果

Targeted delivery of an ADP-ribosylating bacterial toxin into cancer cells

将 ADP 核糖基化细菌毒素靶向递送至癌细胞中

• DOI: 10.1038/srep41252
• 发表时间: 2017
• 期刊: Scientific Reports
• 影响因子: 4.6
• 作者: A.E. Lang;L. Kaiser;C.D. Fichter;S. Lassmann;A. McCluskey;A. Augspach;K. Aktories;G. Schmidt
• 通讯作者: G. Schmidt

Insecticidal Toxin Complexes from Photorhabdus luminescens.

• DOI: 10.1007/82_2016_55
• 发表时间: 2017
• 期刊: Current topics in microbiology and immunology
• 作者: J. Sheets;K. Aktories

The actin and Rho‐modifying toxins PTC3 and PTC5 of Photorhabdus luminescens: enzyme characterization and induction of MAL/SRF‐dependent transcription

Photorhabdus luminescens 的肌动蛋白和 Rhoâ 修饰毒素 PTC3 和 PTC5：酶表征和 MAL/SRFâ 依赖性转录的诱导

• DOI: 10.1111/cmi.12386
• 发表时间: 2015
• 期刊: Cellular Microbiology
• 影响因子: 3.4
• 作者: Pfaumann;A.E. Lang;C. Schwan;G. Schmidt;K. Aktories
• 通讯作者: K. Aktories

Mechanism of Tc toxin action revealed in molecular detail

• DOI: 10.1038/nature13015
• 发表时间: 2014-04-03
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Meusch, Dominic;Gatsogiannis, Christos;Raunser, Stefan
• 通讯作者: Raunser, Stefan