Lipid Dip-Pen Nanolithography for Model Bio-Membrane Systems

用于模型生物膜系统的脂质浸笔纳米光刻

• 批准号: 0723542
• 负责人: Chad Mirkin
• 金额: $ 42万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0723542&HistoricalAwards=false
• 关键词: Lipid; Dip; Pen; Nanolithography; Model

The Analytical and Surface Chemistry Program in the Division of Chemistry will support the collaborative research program of Prof. Mirkin of Northwestern University and Prof. Harald Fuchs of the University of Munster, Germany. This award coordinates with a collaborative award funded by the Deutsche Forschungsgemeinschaft (DFG) through a joint program between the NSF and DFG that jointly funds collaborative projects between US and German investigators.The purpose of this collaborative project is to develop the method of Lipid Dip-Pen Nanolithography (L-DPN) for the fabrication of phospholipid membranes capable of mimicking cellular functions in vitro. The expertise of the Mirkin group in surface biochemistry, DPN and microfabrication will be combined with the Fuchs group expertise in scanning probe microscopy, self organized systems and nano-scale analytics. There is growing evidence that the various properties of biological membranes strongly depend on both two- and three-dimensional heterogeneities. Therefore, it would be useful for modeling cellular functions to have multi-component membrane arrays with ultra-high lateral resolution in which 2 and 3 dimensional interaction can be controlled. Current methods for generating fluid and mechanically stable membrane arrays on surfaces are not optimum for patterning lipids on substrates for reconstitution of proteins. Additionally, encapsulation of materials into supported lipid multilayers at an appropriate scale or with several chemical components remains a significant challenge. DPN is the only currently available technology that may be capable of overcoming these obstacles. L-DPN fabricated patterns will be characterized as a function of the lipid ink composition, substrate chemistry, and environmental conditions in order to determine suitable feature sizes for studying interactions of biological molecules, the supramolecular structures of lipid patterns, and materials which are amenable to integration with phospholipid inks. Once fully developed, these tools will allow biologists, chemists, and others to study biological and chemical interactions at a fundamental level. The project will provide excellent training opportunities to students in a highly multi-disciplinary area, which will include international research experience in Germany and for German students in the US.The award is co-funded by the Office of International Science and Engineering at NSF.

化学系的分析和表面化学计划将支持西北大学米尔金教授和德国芒斯特大学的Harald Fuchs教授的合作研究计划。 This award coordinates with a collaborative award funded by the Deutsche Forschungsgemeinschaft (DFG) through a joint program between the NSF and DFG that jointly funds collaborative projects between US and German investigators.The purpose of this collaborative project is to develop the method of Lipid Dip-Pen Nanolithography (L-DPN) for the fabrication of phospholipid membranes capable of mimicking细胞在体外功能。 Mirkin组在表面生物化学，DPN和微分化方面的专业知识将与Fuchs组扫描探针显微镜，自组织系统和纳米级分析的专业知识相结合。越来越多的证据表明，生物膜的各种特性在很大程度上取决于二维和三维异质性。 因此，对于具有超高侧分辨率的多组分膜阵列进行建模是有用的，其中可以控制2和3维相互作用。在表面上生成流体和机械稳定的膜阵列的当前方法并不是对蛋白质重构的底物进行脂质的构图。此外，将材料以适当的规模或几种化学成分的范围封装到受支持的脂质多层中仍然是一个重大挑战。 DPN是当前唯一能够克服这些障碍的技术。 L-DPN制造的模式将被表征为脂质墨水组成，底物化学和环境条件的函数，以确定用于研究生物分子相互作用的合适特征大小，脂质模式的超分子结构以及与磷脂Inks相结合的材料。一旦完全开发，这些工具将允许生物学家，化学家和其他人在基本水平上研究生物学和化学相互作用。该项目将为高度多学科领域的学生提供极好的培训机会，该培训将包括德国的国际研究经验和美国的德国学生。该奖项由NSF国际科学和工程办公室共同资助。