Collaborative Research: IIBR Instrumentation: The Nanosizer - A new tool for the preparation of arbitrary bioactive surfaces

合作研究：IIBR 仪器：Nanosizer - 用于制备任意生物活性表面的新工具

• 批准号: 2032180
• 负责人: Chad Mirkin
• 金额: $ 45万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2032180&HistoricalAwards=false
• 关键词: Collaborative; Research; IIBR; Instrumentation; Nanosizer

An award is made to Northwestern University and the City University of New York to develop an instrument capable of synthesizing arbitrary 3D nanopatterns of biologically active materials, coined “the Nanosizer.” The development of said instrument, the instrument itself, and the new photochemistry developed, will have wide reaching impacts on trainees, the nearby Chicago and New York City communities, and the broader scientific community. Both Mirkin and Braunschweig groups have previously and will continue to mentor trainees, including undergraduates, graduate students, and postdoctoral fellows, on the design of experiments and communication skills. Importantly, the researchers will lead the experimentation effort and work closely with the PIs on the dissemination of results through high-impact publications, patents, grant reports, and conference presentations. Exposure to all aspects of the research process will provide trainees with the highest probability of success on their paths forward, as proven by the track record of academic and industrial job placement from the two groups. The neighboring Chicago and New York communities stand to benefit from the award, through participation in outreach endeavors. Braunschweig group will participate in the Research Internships in Materials Science and Mirkin members will volunteer with Science In your Community Center with the goal of engaging K-12 students, primarily those from traditionally underrepresented groups, in science and technology. The Nanosizer will make arbitrary 3D nanopatterns of biologically active macromolecules with 100 nm resolution a reality through in situ surface patterning and fully orthogonal biomacromolecule construction. Through a combination of beam-pen lithography with 100 nm pixel resolution, precise multiwavelength irradiation control, and continuous flow of variable reagent streams, the Nanosizer will enable control over the reaction conditions at any 100 nm pixel over a 1 cm2 area. This, in conjunction with the development of orthogonal photochemistries for surface functionalization and synthesis of biomolecules, such as oligopeptides, oligonucleotides, and glycans, will enable the massively parallel generation of biomolecule arrays, with each pixel containing a chemically unique and well-defined molecule. Synthetic interfaces coated with biologically active materials have screening and functional applications in nearly every field of biology. The Nanosizer will provide monumental improvements in batch-to-batch reproducibility, resolution size, feature density, feature quantity, and overall cost in the preparation of such substrates. Results from these studies will be published in peer-reviewed journals, presented at scientific meetings, broadly distributed through commercialization, and incorporated into outreach presentations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

西北大学和纽约城市大学获得了一项奖项，开发一种能够合成生物活性材料的任意 3D 纳米图案的仪器，称为“Nanosizer”。所述仪器的开发、仪器本身以及开发的新光化学将对学员、附近的芝加哥和纽约市社区以及更广泛的科学界产生广泛的影响。米尔金和不伦瑞克研究小组此前已经并将继续为包括本科生、研究生和博士后在内的学员提供实验设计和沟通技巧方面的指导。重要的是，研究人员将领导实验工作，并与 PI 密切合作，通过高影响力的出版物、专利、资助报告和会议演示来传播结果。接触研究过程的各个方面将为学员提供在前进道路上获得成功的最高可能性，这两个小组的学术和工业就业记录证明了这一点。邻近的芝加哥和纽约社区将通过参与外展活动从该奖项中受益。布伦瑞克小组将参加材料科学方面的研究实习，Mirkin 成员将志愿参与社区中心的科学，目标是让 K-12 学生（主要是来自传统上代表性不足的群体的学生）参与科学和技术。 Nanosizer 将通过原位表面图案化和完全正交的生物大分子结构，使具有 100 nm 分辨率的生物活性大分子的任意 3D 纳米图案成为现实。通过将光束笔光刻技术与 100 nm 像素分辨率、精确的多波长照射控制以及可变试剂流的连续流动相结合，Nanosizer 将能够控制 1 cm2 面积上任何 100 nm 像素的反应条件。这与用于表面功能化和生物分子（例如寡肽、寡核苷酸和聚糖）合成的正交光化学的发展相结合，将能够大规模并行生成生物分子阵列，每个像素都包含化学上独特且明确的分子。涂有生物活性材料的合成界面在几乎每个生物学领域都有筛选和功能应用。 Nanosizer 将在批量再现性、分辨率尺寸、特征密度、特征数量以及制备此类基材的总体成本方面提供巨大的改进。这些研究的结果将发表在同行评审的期刊上，在科学会议上发表，通过商业化广泛分发，并纳入外展演示中。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Photopolymerized Features via Beam Pen Lithography as a Novel Tool for the Generation of Large Area Protein Micropatterns

通过束笔光刻的光聚合特征作为生成大面积蛋白质微图案的新工具

• DOI: 10.1002/smll.202105998
• 发表时间: 2022
• 期刊: Small
• 影响因子: 13.3
• 作者: Zhang, Xinpeng;Ding, Shaowei;Magoline, Jared;Ivankin, Andrey;Mirkin, Chad A.
• 通讯作者: Mirkin, Chad A.