Collaborative Research: IIBR Instrumentation: The Nanosizer - A new tool for the preparation of arbitrary bioactive surfaces

合作研究：IIBR 仪器：Nanosizer - 用于制备任意生物活性表面的新工具

• 批准号: 2032180
• 负责人: Chad Mirkin
• 金额: $ 45万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2032180&HistoricalAwards=false
• 关键词: Collaborative; Research; IIBR; Instrumentation; Nanosizer

An award is made to Northwestern University and the City University of New York to develop an instrument capable of synthesizing arbitrary 3D nanopatterns of biologically active materials, coined “the Nanosizer.” The development of said instrument, the instrument itself, and the new photochemistry developed, will have wide reaching impacts on trainees, the nearby Chicago and New York City communities, and the broader scientific community. Both Mirkin and Braunschweig groups have previously and will continue to mentor trainees, including undergraduates, graduate students, and postdoctoral fellows, on the design of experiments and communication skills. Importantly, the researchers will lead the experimentation effort and work closely with the PIs on the dissemination of results through high-impact publications, patents, grant reports, and conference presentations. Exposure to all aspects of the research process will provide trainees with the highest probability of success on their paths forward, as proven by the track record of academic and industrial job placement from the two groups. The neighboring Chicago and New York communities stand to benefit from the award, through participation in outreach endeavors. Braunschweig group will participate in the Research Internships in Materials Science and Mirkin members will volunteer with Science In your Community Center with the goal of engaging K-12 students, primarily those from traditionally underrepresented groups, in science and technology. The Nanosizer will make arbitrary 3D nanopatterns of biologically active macromolecules with 100 nm resolution a reality through in situ surface patterning and fully orthogonal biomacromolecule construction. Through a combination of beam-pen lithography with 100 nm pixel resolution, precise multiwavelength irradiation control, and continuous flow of variable reagent streams, the Nanosizer will enable control over the reaction conditions at any 100 nm pixel over a 1 cm2 area. This, in conjunction with the development of orthogonal photochemistries for surface functionalization and synthesis of biomolecules, such as oligopeptides, oligonucleotides, and glycans, will enable the massively parallel generation of biomolecule arrays, with each pixel containing a chemically unique and well-defined molecule. Synthetic interfaces coated with biologically active materials have screening and functional applications in nearly every field of biology. The Nanosizer will provide monumental improvements in batch-to-batch reproducibility, resolution size, feature density, feature quantity, and overall cost in the preparation of such substrates. Results from these studies will be published in peer-reviewed journals, presented at scientific meetings, broadly distributed through commercialization, and incorporated into outreach presentations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

美国西北大学（Northwestern University）和纽约城市大学（City University of New York）获得了一个奖项，以开发一种能够合成任意3D纳米生物活性材料的仪器，该仪器被称为“纳米尺寸仪”（Nanosizer）。上述仪器的发展、仪器本身以及所开发的新的光化学，将对受训人员、附近的芝加哥和纽约社区以及更广泛的科学界产生广泛的影响。米尔金和布伦瑞克的研究小组已经并将继续指导受训者，包括本科生、研究生和博士后，在实验设计和沟通技巧方面。重要的是，研究人员将领导实验工作，并与pi密切合作，通过高影响力的出版物、专利、拨款报告和会议报告传播结果。接触研究过程的各个方面将为受训者提供最高的成功可能性，正如两组的学术和工业就业安置记录所证明的那样。邻近的芝加哥和纽约社区将通过参与外展活动而从该奖项中受益。布伦瑞克大学的学生将参加材料科学的研究实习，米尔金大学的学生将在社区中心做科学志愿者，目的是吸引K-12学生，主要是那些传统上代表性不足的群体，参与科学和技术。Nanosizer将通过原位表面图像化和完全正交的生物大分子构建，使具有100纳米分辨率的生物活性大分子的任意3D纳米模式成为现实。通过结合具有100 nm像素分辨率的束笔光刻，精确的多波长辐照控制和可变试剂流的连续流动，nanosize将能够控制1 cm2面积上任何100 nm像素的反应条件。这与用于表面功能化和合成生物分子（如寡肽、寡核苷酸和聚糖）的正交光化学的发展相结合，将使生物分子阵列的大规模平行生成成为可能，每个像素包含一个化学上独特且定义良好的分子。生物活性材料包覆的合成界面在生物学的各个领域都有筛选和功能应用。纳米尺寸仪将在批对批的重现性、分辨率大小、特征密度、特征数量和制备这种基板的总成本方面提供巨大的改进。这些研究的结果将发表在同行评议的期刊上，在科学会议上提出，通过商业化广泛分发，并纳入外联报告。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Photopolymerized Features via Beam Pen Lithography as a Novel Tool for the Generation of Large Area Protein Micropatterns

通过束笔光刻的光聚合特征作为生成大面积蛋白质微图案的新工具

• DOI: 10.1002/smll.202105998
• 发表时间: 2022
• 期刊: Small
• 影响因子: 13.3
• 作者: Zhang, Xinpeng;Ding, Shaowei;Magoline, Jared;Ivankin, Andrey;Mirkin, Chad A.
• 通讯作者: Mirkin, Chad A.