CAREER: Understanding Process-Induced Damage in Laser-Assisted Cell Direct Writing - Bridging Manufacturing Science and Biomedical Research

本项目的研究目的是阐明激光辅助活细胞直写中过程诱导的热机械负荷的大小和持续时间对细胞损伤的影响。利用激光辅助活细胞直写技术，本研究将1)模拟细胞液滴形成和着陆过程中过程诱导的热机械载荷的大小和持续时间；2)通过了解细胞损伤/存活与过程诱导的热机械载荷大小和持续时间之间的机制关系来模拟细胞转移后的活力；以及3)基于细胞死亡测量来校准和验证细胞存活的机械模型。教育目标是通过生物医学制造创新促进工程学。主要的教育活动包括通过电子博物馆向高中生提供外展服务，加工身体：年轻的机械师，包括青年调查员视频系列，以及为生物医学制造的高等教育开发和传播教育材料。如果成功，这项研究将有助于理解激光辅助细胞液滴形成和着陆过程的动力学，以及过程诱导的热机械负荷对细胞损伤/存活的影响。由此产生的机械细胞存活模型将阐明对基于喷射的直接写入所导致的过程诱导的细胞损伤的理解。这项研究的更广泛影响是多方面的。细胞直接写入的安全和高效实现使其在器官打印和基于细胞的产品的快速成型方面得到了广泛的应用。社会将受益于这些新的按需器官制造和细胞种子相关的健康技术，这项研究将促进制造科学和生物医学研究之间的研究合作。教育与生物医学制造研究的结合将吸引代表不足的少数族裔学生，并将工程学推广到更广泛的人群中。

The research objective of this Faculty Early Career Development (CAREER) Program project is to elucidate the effects of process-induced thermomechanical loading magnitude and duration on cell damage in laser-assisted living cell direct writing. Using a laser-assisted living cell direct-write technology, this research will 1) model the process-induced thermomechanical loading magnitude and duration during cell droplet formation and landing processes; 2) model the cell post-transfer viability by understanding the mechanistic correlation between cell damage/viability and process-induced thermomechanical loading magnitude and duration; and 3) calibrate and validate the mechanistic cell viability model based on cell death measurements. The educational objective is to promote engineering through biomedical manufacturing innovations. Key education activities include providing outreach to high school students through an e-museum, Machining the Body: Young Machinist, including a Young Investigator Video series and developing and disseminating educational materials for high education in biomedical manufacturing.If successful, this study will contribute towards an understanding of the dynamics of laser-assisted cell droplet formation and landing processes and the effects of process-induced thermomechanical loading on cell damage/viability. The resulting mechanistic cell viability model will elucidate an understanding of process-induced cell damage caused by jet-based direct writing. Broader impacts of this study are multifaceted. The safe and efficient implementation of cell direct writing enables its wide application in both organ printing and rapid prototyping of cell-based products. Society will benefit from these new on-demand organ manufacturing and cell seeding-related health technologies, and this study will foster research collaboration between manufacturing science and biomedical research. The integration of education with biomedical manufacturing research will attract underrepresented and minority students and promote engineering to a broader population.