CAREER: Understanding Process-Induced Damage in Laser-Assisted Cell Direct Writing - Bridging Manufacturing Science and Biomedical Research

本学院早期职业发展（CAREER）计划项目的研究目标是阐明激光辅助活细胞直写中过程诱导的热机械负载幅度和持续时间对细胞损伤的影响。利用激光辅助活细胞直写技术，本研究将1）模拟细胞液滴形成和着陆过程中的过程诱导的热机械载荷大小和持续时间; 2）通过理解细胞损伤/存活率与过程诱导的热机械载荷大小和持续时间之间的机械相关性来模拟细胞转移后的存活率;以及3）基于细胞死亡测量来校准和验证机械细胞存活力模型。教育目标是通过生物医学制造创新促进工程。主要的教育活动包括通过一个电子博物馆向高中生提供外展服务，该博物馆名为“加工身体：青年机械师，包括一个青年调查员视频系列，并为生物医学制造业的高等教育开发和传播教育材料。这项研究将有助于了解激光辅助细胞液滴形成和着陆过程的动力学以及过程的影响，诱导的热机械负荷对细胞损伤/活力的影响。由此产生的机械细胞活力模型将阐明由基于喷射的直接写入引起的过程诱导的细胞损伤的理解。这项研究的更广泛影响是多方面的。细胞直写的安全和高效实现使其在器官打印和基于细胞的产品的快速原型制作中得到广泛应用。社会将受益于这些新的按需器官制造和细胞接种相关的健康技术，这项研究将促进制造科学和生物医学研究之间的研究合作。将教育与生物医学制造研究相结合将吸引代表性不足的少数民族学生，并将工程学推广到更广泛的人群。

The research objective of this Faculty Early Career Development (CAREER) Program project is to elucidate the effects of process-induced thermomechanical loading magnitude and duration on cell damage in laser-assisted living cell direct writing. Using a laser-assisted living cell direct-write technology, this research will 1) model the process-induced thermomechanical loading magnitude and duration during cell droplet formation and landing processes; 2) model the cell post-transfer viability by understanding the mechanistic correlation between cell damage/viability and process-induced thermomechanical loading magnitude and duration; and 3) calibrate and validate the mechanistic cell viability model based on cell death measurements. The educational objective is to promote engineering through biomedical manufacturing innovations. Key education activities include providing outreach to high school students through an e-museum, Machining the Body: Young Machinist, including a Young Investigator Video series and developing and disseminating educational materials for high education in biomedical manufacturing.If successful, this study will contribute towards an understanding of the dynamics of laser-assisted cell droplet formation and landing processes and the effects of process-induced thermomechanical loading on cell damage/viability. The resulting mechanistic cell viability model will elucidate an understanding of process-induced cell damage caused by jet-based direct writing. Broader impacts of this study are multifaceted. The safe and efficient implementation of cell direct writing enables its wide application in both organ printing and rapid prototyping of cell-based products. Society will benefit from these new on-demand organ manufacturing and cell seeding-related health technologies, and this study will foster research collaboration between manufacturing science and biomedical research. The integration of education with biomedical manufacturing research will attract underrepresented and minority students and promote engineering to a broader population.