Modeling the Formation of Highly Aligned Texture on the Inner Surface of Semi-Permeable Axonal Guidance Hollow Fiber Membranes

这项资助为理解使用湿相转化过程在半渗透性轴突引导中空纤维膜（HFM）的内表面上高度对齐的纹理的形成机制提供了支持。这种理解对于开发可再生和高效的组织再生支架至关重要。本研究的目的是模拟湿相转化过程中的HFM槽的形成机制，并建立一个灵敏度之间的关系制造槽的几何形状和轴突生长速率。主要研究活动包括设计和建造具有完全传感和控制能力的实验性相反转装置，了解沟槽形成机理，对所制造的HFM宏观几何和微观几何进行建模，验证沟槽形成的建模方法，并通过背根神经节外植体再生试验测试沟几何形状对轴突生长的敏感性。这项研究预计将产生以下知识HFM内表面凹槽形成建模的物理推理、HFM宏观和微观几何形状对轴突生长有效性的敏感性、以及用于基于相反转的HFM制造的传感器集成装置的更好设计。这项研究将是一个开创性的尝试，了解，建模，并制造高度对齐的内表面上的凹槽的HFM。这项研究将提高临床应用的轴突生长，刺激生物制造研究的科学理解，促进生物制造的教学和培训，扩大代表性不足的群体的参与，并加强克莱姆森大学生物制造研究和教育的基础设施，并向全国进行详细的推广。

This grant provides support for the effort to understand the formation mechanism of highly aligned textures on the inner surface of semi-permeable axonal guidance hollow fiber membranes (HFMs) using a wet phase inversion process. Such an understanding is critical in developing reproducible and highly effective scaffolds for tissue regeneration. The objectives of this research are to model the HFM groove formation mechanism during the wet phase inversion process and establish a sensitivity relationship between the fabricated groove geometry and axonal outgrowth rate. Key research activities will include designing and building an experimental phase inversion apparatus with full sensing and controlling capability, understanding the groove formation mechanism, modeling the fabricated HFM macrogeometry and microgeometry, verifying the modeling approaches for groove formation, and testing the sensitivity of groove geometry on axonal outgrowth via a dorsal root ganglion explant regeneration assay.This study is expected to generate knowledge on physical reasoning in modeling HFM inner surface groove formation, sensitivity of HFM macroscopic and microscopic geometries on axonal outgrowth effectiveness, and a better design for a sensor integrated apparatus for phase inversion based HFM fabrication. This research will be a pioneering attempt to understand, model, and fabricate highly aligned grooves on the inner surface of HFMs. This study will improve the axonal outgrowth for clinical applications, stimulate scientific understanding of biomanufacturing research, promote teaching and training in biomanufacturing, broaden participation of underrepresented groups, and enhance the infrastructure for biomanufacturing research and education at Clemson University with a detailed outreach to the whole nation.