Scalable Laser Printing of Three-Dimensional Living Tissue Constructs

三维活组织结构的可扩展激光打印

• 批准号: 1537956
• 负责人: Yong Huang
• 金额: $ 30万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1537956&HistoricalAwards=false
• 关键词: Scalable; Laser; Printing; Three; Dimensional

The rising success rate of transplants has resulted in a critical need for more tissues and organs. Approximately 95,000 people are on the waiting list for new organs in the US alone, and some die every day waiting for transplants. Organ printing provides a promising solution to the problem of organ donor shortage. This award supports fundamental research to generate knowledge that enables the scale up of a jet-based, orifice-free laser bioprinting technique for organ printing. Results from this research will enable the wider use of laser printing for the fabrication of living tissue constructs for implantation. In addition, this award will promote the development of bioprinting technologies for cell and organ printing and provide three-dimensional engineered tissues for cell behavior and pharmacokinetics studies.In this new laser bioprinting technique, an optical scanner will be incorporated into the laser beam delivery system to optically guide laser pulses to efficiently transfer bioinks of a ribbon instead of mechanically moving the ribbon. The objective of this research is to understand the effects of the interaction of laser pulses-induced adjacent fluid jets on the jettability and printability during optical scanner-enabled laser-induced forward transfer bioprinting. The jettability will be evaluated based on whether two adjacent well-defined jets can be formed during jet formation, and the printability will be assessed based on whether a well-defined line can be formed from well-defined jets. The formation of each laser-induced jet will be modeled using the existing homogenous nucleation-based phase explosion theory. The governing fluid continuity and Navier-Stokes equations for droplet formation under the jet interaction will be solved numerically using a finite element method with mixed interpolation for spatial discretization as well as an implicit finite difference method for time integration. In order to derive a reduced system of equations and boundary conditions containing the governing physics, a scale analysis of the governing equations and interfacial conditions will be performed. The scale analysis results will be used to validate the numerical simulation on the onset of instability (droplet formation) under the effects of two adjacent jets. The resulting knowledge of the interaction of adjacent jets will be used to predict the influences of printing conditions such as the scanning speed of optical scanner on the jettability and printability and further validated during the laser bioprinting of cellular tubes.

移植成功率的提高导致了对更多组织和器官的迫切需求。仅在美国，就有大约95，000人在等待新器官的名单上，每天都有一些人在等待移植时死亡。器官打印为解决器官捐献者短缺问题提供了一个很有前途的解决方案。该奖项支持基础研究，以产生知识，从而能够扩大基于喷射的，无牺牲品的激光生物打印技术用于器官打印。这项研究的结果将使激光打印更广泛地用于制造用于植入的活组织结构。此外，该奖项还将促进用于细胞和器官打印的生物打印技术的发展，并为细胞行为和药代动力学研究提供三维工程组织。在这种新的激光生物打印技术中，光学扫描仪将被纳入激光束传输系统，以光学方式引导激光脉冲有效地转移色带的生物墨水，而不是机械地移动色带。本研究的目的是了解在光学扫描仪使能的激光诱导前向转移生物打印过程中，激光脉冲诱导的相邻流体射流的相互作用对喷射性和可打印性的影响。将基于在喷射形成期间是否可以形成两个相邻的明确限定的喷射来评价喷射，并且将基于是否可以从明确限定的喷射形成明确限定的线来评估适印性。每个激光诱导射流的形成将使用现有的均匀成核相爆炸理论建模。在射流相互作用下液滴形成的控制流体连续性和Navier-Stokes方程将使用混合插值的有限元法进行空间离散化以及隐式有限差分法进行时间积分来数值求解。为了推导出包含控制物理的简化方程组和边界条件，将对控制方程和界面条件进行尺度分析。尺度分析的结果将被用来验证数值模拟的不稳定性（液滴形成）的两个相邻的射流的影响下的发病。相邻射流相互作用的所得知识将用于预测打印条件（例如光学扫描仪的扫描速度）对射流和可打印性的影响，并在细胞管的激光生物打印期间进一步验证。