EAGER: 3D Printing of Aligned Muscle Fibers for Thick Structured Meat Production
EAGER:用于厚结构肉生产的对齐肌肉纤维的 3D 打印
基本信息
- 批准号:2233814
- 负责人:
- 金额:$ 30万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-15 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The continued growth of human populations with declined resources has imposed a significant challenge to affordable and sustainable foods and nutrition. One resource-efficient solution is cultured meat, which is genuine animal meat produced by cultivating animal cells directly using a bioreactor. Though scaffold-based technologies have demonstrated the feasibility of making minced or unstructured meat products, such technologies are limited and cannot produce thick structured meat. This EArly-concept Grant for Exploratory Research (EAGER) supports fundamental research that aims to establish a scaffold-free 3D embedded bioprinting technology to enable the production of cultured meat with centimeter thick and structured features. The study will explore the alignment and fusion of myoblasts during embedded meat printing in a gelatin composite-based cellular matrix bath. The results will catalyze future scale-up production of thick structured cuts of cultured meat and promote cellular agriculture as the future of complementary food production for the benefits of sustainability, public health, and animal welfare. The project will also stimulate science-based bioprinting research to advance cultured-meat manufacture and broaden the participation of underrepresented students in crosscutting STEM fields via the bioprinting study.The objective of this research is to understand the effects of extrusion-induced shear force and post-printing tension on the formation of aligned muscle fibers from myoblasts during embedded printing of thick multicellular structured meat-like tissues. Specifically, myoblasts will be printed in an embedded manner, aligned, and stretched for myoblast fusion to be myotubes and further matured as myofibers in the gelatin composite-based yield-stress matrix bath. The printed sacrificial bioink will then be removed to form perfusable channels. While embedded 3D printing of myoblasts and adipocyte progenitor cells will enable printed tissues to be structured, the perfusable channels and capillaries self-assembled by endothelial and adipose-derived stem cells will enable the printed tissues to grow thick. Theoretically, the effect of shear force on the myoblast alignment during printing will be computationally modeled using the Eulerian formulation and myoblasts will be macroscopically treated as a linear elastic solid in the myoblast bioink. The modeling results will be validated with the orientation of the printed myoblasts. Next, the cyclic tension-induced effect on myoblast fusion will be investigated during the culturing of the printed meat-like tissues in a customized bioreactor, and the printed tissues will be perfused via the channels. The resulting meat-like tissues will be characterized in terms of vascularization, myoblast differentiation as well as myotube and myofiber formation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
资源减少的人类人口持续增长,对负担得起和可持续的食物和营养提出了重大挑战。一种资源高效的解决方案是培养肉,这是通过直接使用生物反应器培养动物细胞生产的真正的动物肉。尽管基于脚手架的技术已经证明了制造碎肉或非结构化肉类产品的可行性,但这种技术是有限的,无法生产厚重的结构性肉类。这一早期概念探索性研究补助金(AGERGE)支持旨在建立无支架3D嵌入式生物打印技术的基础研究,从而能够生产出具有厘米厚和结构特征的养殖肉类。这项研究将探索成肌细胞在明胶复合材料细胞基质浴中嵌入肉类印花过程中的排列和融合。这一结果将促进未来规模化养殖肉类的规模化生产,并促进细胞农业作为补充食品生产的未来,以促进可持续发展、公共卫生和动物福利。该项目还将促进基于科学的生物印刷研究,以促进培养肉类的制造,并通过生物印刷研究扩大代表不足的学生在横切STEM领域的参与。本研究的目的是了解挤压诱导的剪切力和印刷后张力对成肌细胞在厚重的多细胞结构肉类组织嵌入印刷过程中形成排列的肌肉纤维的影响。具体地说,成肌细胞将以嵌入的方式打印、排列和拉伸,以便成肌细胞融合成肌管,并在明胶复合材料的屈服应力基质浴中进一步成熟为肌纤维。然后,印刷的牺牲生物墨水将被移除,以形成可灌装的通道。虽然成肌细胞和脂肪细胞前体细胞的嵌入式3D打印将使打印组织结构化,但由内皮干细胞和脂肪来源的干细胞自组装的可灌流的通道和毛细血管将使打印组织变厚。从理论上讲,印刷过程中剪切力对成肌细胞排列的影响将使用欧拉公式进行计算建模,成肌细胞将被宏观地视为成肌细胞生物墨水中的线弹性固体。建模结果将与打印的成肌细胞的取向进行验证。接下来,在定制的生物反应器中培养打印的肉样组织时,将研究循环张力对成肌细胞融合的影响,打印的组织将通过通道进行灌流。由此产生的肉样组织将在血管形成、成肌细胞分化以及肌管和肌纤维形成方面具有特征。该奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Laponite nanoclay-modified sacrificial composite ink for perfusable channel creation via embedded 3D printing
- DOI:10.1016/j.compositesb.2023.110851
- 发表时间:2023-08
- 期刊:
- 影响因子:0
- 作者:B. Ren;Kaidong Song;Yunxia Chen;W. Murfee;Yong Huang
- 通讯作者:B. Ren;Kaidong Song;Yunxia Chen;W. Murfee;Yong Huang
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Yong Huang其他文献
Insight into the production of phenol from co‐pyrolysis of cellulose and sodium borohydride
深入了解纤维素和硼氢化钠共热解生产苯酚
- DOI:
10.1002/bbb.2370 - 发表时间:
2022-04 - 期刊:
- 影响因子:0
- 作者:
Yanqian Gu;Shasha Liu;Gang Wu;Yinlong Wu;Shoujun Zhang;Yong Huang;Shu Zhang;Hong Zhang - 通讯作者:
Hong Zhang
Can Reflective Interventions Improve Students’ Academic Achievement: A Meta-analysis
反思性干预能否提高学生的学业成绩:荟萃分析
- DOI:
10.1016/j.tsc.2023.101373 - 发表时间:
2023 - 期刊:
- 影响因子:3.7
- 作者:
N. Zhai;Yong Huang;Xiaomei Ma;Jing Chen - 通讯作者:
Jing Chen
A multiscale Galerkin method for second-order boundary value problems of Fredholm integro-differential equation
Fredholm积分微分方程二阶边值问题的多尺度伽辽金法
- DOI:
10.1016/j.cam.2015.06.020 - 发表时间:
2015-12 - 期刊:
- 影响因子:2.4
- 作者:
Yong Huang;Haiwu Rong;Tingting Wu;Taishan Zeng - 通讯作者:
Taishan Zeng
Optimization of Long-wavelength InAs/GaSb Superlattice Photodiodes Grown by MOCVD
MOCVD 生长的长波长 InAs/GaSb 超晶格光电二极管的优化
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:2.6
- 作者:
Yu Zhao;Yan Teng;Xiujun Hao;Qihua Wu;Jingjun Miao;Xin Li;Min Xiong;Yong Huang - 通讯作者:
Yong Huang
Serum NOX2 as a new biomarker candidate for HBV-related disorders
血清 NOX2 作为 HBV 相关疾病的新候选生物标志物
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:2.2
- 作者:
Yuan Xiong;Yuanyuan Ye;Li Pu;Yahui Xiong;Jinju Mao;Yong Huang;Weixian Chen;Bo Wang - 通讯作者:
Bo Wang
Yong Huang的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Yong Huang', 18)}}的其他基金
Pore Formation and Polymer Thermal Debinding during Vapor-Induced Phase Separation-Enabled Metal Printing
蒸汽诱导相分离金属打印过程中的孔形成和聚合物热脱脂
- 批准号:
2315811 - 财政年份:2023
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Manufacturing USA: Study of Self-Supporting Nanoclay as Internal Scaffold Material for Printing of Skeletal Tissue Constructs
美国制造:自支撑纳米粘土作为骨骼组织结构打印内部支架材料的研究
- 批准号:
1762941 - 财政年份:2018
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
GOALI: Printing of Heterogeneous Tissue Constructs from Reactive Biomaterials using Intersecting Jets
GOALI:使用相交喷射机打印反应性生物材料的异质组织结构
- 批准号:
1634755 - 财政年份:2016
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Scalable Laser Printing of Three-Dimensional Living Tissue Constructs
三维活组织结构的可扩展激光打印
- 批准号:
1537956 - 财政年份:2015
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Workshop: Environmental Implications of Additive Manufacturing; Arlington, Virginia; October 14-15, 2014
研讨会:增材制造的环境影响;
- 批准号:
1450529 - 财政年份:2014
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Collaborative Research: Understanding Machining-Induced Influences to Ultra-Fine Grained Pure Titanium for Biomedical Applications
合作研究:了解机械加工对生物医学应用超细晶纯钛的影响
- 批准号:
1404926 - 财政年份:2014
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
NSF Workshop on Frontiers of Additive Manufacturing Research and Education; Arlington, Virginia; 11-12 July 2013
NSF 增材制造研究和教育前沿研讨会;
- 批准号:
1339027 - 财政年份:2013
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
CAREER: Understanding Process-Induced Damage in Laser-Assisted Cell Direct Writing - Bridging Manufacturing Science and Biomedical Research
职业:了解激光辅助细胞直写过程中引起的损伤 - 连接制造科学和生物医学研究
- 批准号:
1321271 - 财政年份:2013
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Fabrication of Double-Layer Cellular Spheroid using Acoustic Excitation-Assisted Compound Jetting
使用声激励辅助复合喷射制备双层细胞球体
- 批准号:
1314834 - 财政年份:2013
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Collaborative Research: Laser-Assisted Orifice-Free Fabrication of Viscous Alginate Microspheres
合作研究:激光辅助无孔制造粘性海藻酸盐微球
- 批准号:
1314830 - 财政年份:2013
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
相似国自然基金
锰酸锂基复合气凝胶的3D打印构筑及其提锂机制研究
- 批准号:JCZRLH202500778
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
3D细胞球来源的凋亡小体修饰间充质干细胞的制备及“内外兼修”策略的构建用于脊髓损伤修复的作用机制研究
- 批准号:QN25H060008
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
智能3D超微血管成像联合实时剪切波弹性评估胎盘功能对高血压孕妇子痫前期的预测效能分析
- 批准号:
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
3D打印楔形梯度多孔支架的优化构建及促进HTO术后骨再生修复的实验研究
- 批准号:
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
基于3D Slicer的颅内动脉瘤破裂风险评估机器学习模型开发及临床推广应用
- 批准号:
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
3D打印PH-GBS@CCP复合支架诱导骨肉瘤铜死亡及增效抗PD-1治疗的作用机制研究
- 批准号:
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
双重固化3D打印连续纤维C/C复合材料成型与渗碳致密化机理研究
- 批准号:2025JJ60269
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
载椎体骨髓干细胞外泌体3D打印n-HA/PA66生物支架的研制及促脊柱融合机制研究
- 批准号:2025JJ80409
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
基于微流控技术的3D细胞培养体系构建及在乳腺癌耐药机制中应用研究
- 批准号:2025JJ70487
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
多级结构仿生3D打印生物陶瓷调控
PI3K/Akt信号通路介导细胞应激反应促
进血管化骨再生的作用机制研究
- 批准号:
- 批准年份:2025
- 资助金额:10.0 万元
- 项目类别:省市级项目
相似海外基金
3D Printing Proteins for Continuous Flow Biocatalysis and Bioabsorbtion
用于连续流生物催化和生物吸收的 3D 打印蛋白质
- 批准号:
2753054 - 财政年份:2026
- 资助金额:
$ 30万 - 项目类别:
Studentship
Optimisation of Buildable Structures for 3D Concrete Printing
3D 混凝土打印可建造结构的优化
- 批准号:
DP240101708 - 财政年份:2024
- 资助金额:
$ 30万 - 项目类别:
Discovery Projects
I-Corps: Translation potential of 3D electronics manufacturing by integrated 3D printing and freeform laser induction
I-Corps:通过集成 3D 打印和自由形式激光感应实现 3D 电子制造的转化潜力
- 批准号:
2412186 - 财政年份:2024
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
I-Corps: Translation potential of stereolithography 3D printing to create soft elastomers
I-Corps:立体光刻 3D 打印制造软弹性体的转化潜力
- 批准号:
2414710 - 财政年份:2024
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Recycling of platinum electrodes demonstrating particulate electrochemical printing - PEP 3d Pt
铂电极的回收展示了颗粒电化学印刷 - PEP 3d Pt
- 批准号:
2905755 - 财政年份:2024
- 资助金额:
$ 30万 - 项目类别:
Studentship
FMSG: Cyber: 3D Printing of Holographic Optical Processors
FMSG:网络:全息光学处理器的 3D 打印
- 批准号:
2328362 - 财政年份:2024
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
CAREER: Frequency Agile Real-Time Reconfigurable RF Analog Co-Processor Design Leveraging Engineered Nanoparticle and 3D Printing
职业:利用工程纳米颗粒和 3D 打印进行频率捷变实时可重构射频模拟协处理器设计
- 批准号:
2340268 - 财政年份:2024
- 资助金额:
$ 30万 - 项目类别:
Continuing Grant
Designing metallic glass structures for damage tolerance via 3D printing
通过 3D 打印设计金属玻璃结构以实现损伤容限
- 批准号:
DP240101127 - 财政年份:2024
- 资助金额:
$ 30万 - 项目类别:
Discovery Projects
Integrated Framework for Cooperative 3D Printing: Uncertainty Quantification, Decision Models, and Algorithms
协作 3D 打印的集成框架:不确定性量化、决策模型和算法
- 批准号:
2329739 - 财政年份:2024
- 资助金额:
$ 30万 - 项目类别:
Standard Grant