EAGER: 3D Printing of Aligned Muscle Fibers for Thick Structured Meat Production
EAGER:用于厚结构肉生产的对齐肌肉纤维的 3D 打印
基本信息
- 批准号:2233814
- 负责人:
- 金额:$ 30万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-15 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The continued growth of human populations with declined resources has imposed a significant challenge to affordable and sustainable foods and nutrition. One resource-efficient solution is cultured meat, which is genuine animal meat produced by cultivating animal cells directly using a bioreactor. Though scaffold-based technologies have demonstrated the feasibility of making minced or unstructured meat products, such technologies are limited and cannot produce thick structured meat. This EArly-concept Grant for Exploratory Research (EAGER) supports fundamental research that aims to establish a scaffold-free 3D embedded bioprinting technology to enable the production of cultured meat with centimeter thick and structured features. The study will explore the alignment and fusion of myoblasts during embedded meat printing in a gelatin composite-based cellular matrix bath. The results will catalyze future scale-up production of thick structured cuts of cultured meat and promote cellular agriculture as the future of complementary food production for the benefits of sustainability, public health, and animal welfare. The project will also stimulate science-based bioprinting research to advance cultured-meat manufacture and broaden the participation of underrepresented students in crosscutting STEM fields via the bioprinting study.The objective of this research is to understand the effects of extrusion-induced shear force and post-printing tension on the formation of aligned muscle fibers from myoblasts during embedded printing of thick multicellular structured meat-like tissues. Specifically, myoblasts will be printed in an embedded manner, aligned, and stretched for myoblast fusion to be myotubes and further matured as myofibers in the gelatin composite-based yield-stress matrix bath. The printed sacrificial bioink will then be removed to form perfusable channels. While embedded 3D printing of myoblasts and adipocyte progenitor cells will enable printed tissues to be structured, the perfusable channels and capillaries self-assembled by endothelial and adipose-derived stem cells will enable the printed tissues to grow thick. Theoretically, the effect of shear force on the myoblast alignment during printing will be computationally modeled using the Eulerian formulation and myoblasts will be macroscopically treated as a linear elastic solid in the myoblast bioink. The modeling results will be validated with the orientation of the printed myoblasts. Next, the cyclic tension-induced effect on myoblast fusion will be investigated during the culturing of the printed meat-like tissues in a customized bioreactor, and the printed tissues will be perfused via the channels. The resulting meat-like tissues will be characterized in terms of vascularization, myoblast differentiation as well as myotube and myofiber formation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
人口持续增长,资源减少,对负担得起和可持续的粮食和营养构成了重大挑战。一种资源节约型的解决方案是培养肉,这是通过使用生物反应器直接培养动物细胞生产的真正的动物肉。虽然基于支架的技术已经证明了制造切碎或非结构化肉类产品的可行性,但这些技术是有限的,不能生产厚的结构化肉类。EARLY概念探索性研究资助(EAGER)支持基础研究,旨在建立无支架3D嵌入式生物打印技术,以生产具有厘米厚和结构特征的培养肉。该研究将探讨在明胶复合物为基础的细胞基质浴中嵌入式肉类打印过程中成肌细胞的排列和融合。研究结果将促进未来培养肉厚结构切片的规模化生产,并促进细胞农业作为未来补充食品生产的可持续性,公共卫生和动物福利。该项目还将刺激基于科学的生物打印研究,以推进培养肉制造,并通过生物打印研究扩大代表性不足的学生在交叉STEM领域的参与。本研究的目的是了解在嵌入式打印厚多细胞结构的肉样组织期间,挤压诱导的剪切力和打印后张力对成肌细胞形成对齐肌纤维的影响。具体地,成肌细胞将以包埋方式打印、对齐和拉伸,以使成肌细胞融合为肌管,并在基于明胶复合物的屈服应力基质浴中进一步成熟为肌纤维。然后将去除打印的牺牲生物墨水以形成可灌注通道。虽然成肌细胞和脂肪细胞祖细胞的嵌入式3D打印将使打印的组织结构化,但由内皮和脂肪源性干细胞自组装的可灌注通道和毛细血管将使打印的组织变厚。理论上,在打印期间剪切力对成肌细胞排列的影响将使用欧拉公式进行计算建模,并且成肌细胞将在成肌细胞生物墨水中被宏观地视为线性弹性固体。将使用打印的成肌细胞的方向来验证建模结果。接下来,在定制的生物反应器中培养打印的肉样组织期间,将研究循环张力诱导对成肌细胞融合的影响,并且打印的组织将通过通道灌注。该奖项反映了NSF的法定使命,并通过使用基金会的智力价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Laponite nanoclay-modified sacrificial composite ink for perfusable channel creation via embedded 3D printing
- DOI:10.1016/j.compositesb.2023.110851
- 发表时间:2023-08
- 期刊:
- 影响因子:0
- 作者:B. Ren;Kaidong Song;Yunxia Chen;W. Murfee;Yong Huang
- 通讯作者:B. Ren;Kaidong Song;Yunxia Chen;W. Murfee;Yong Huang
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Yong Huang其他文献
Navigation of Chang’E-2 asteroid exploration mission and the minimum distance estimation during its fly-by of Toutatis
嫦娥二号小行星探测任务导航及飞越图塔蒂斯过程中的最小距离估算
- DOI:
10.1016/j.asr.2014.10.005 - 发表时间:
2015 - 期刊:
- 影响因子:2.6
- 作者:
Jianfeng Cao;Yong Liu;Songjie Hu;Lei Liu;Geshi Tang;Yong Huang;Peijia Li - 通讯作者:
Peijia Li
Simulation of groundwater flow in fractured rocks using a coupled model based on the method of domain decomposition
基于域分解方法的耦合模型模拟裂隙岩体中地下水流
- DOI:
10.1007/s12665-014-3184-y - 发表时间:
2014-03 - 期刊:
- 影响因子:2.8
- 作者:
Yong Huang;Zhifang Zhou;Jinguo Wang;Zhi Dou - 通讯作者:
Zhi Dou
Effect of UV-B on growth and development and secondary metabolism of flue-cured tobacco: Effect of UV-B on growth and development and secondary metabolism of flue-cured tobacco
UV-B对烤烟生长发育和次生代谢的影响: UV-B对烤烟生长发育和次生代谢的影响
- DOI:
10.3724/sp.j.1011.2009.00140 - 发表时间:
2009 - 期刊:
- 影响因子:0
- 作者:
Yong Huang;Ji;Mingmei Zheng;Hong;Ning Zhang - 通讯作者:
Ning Zhang
Dielectric properties of hybrid perovskites and drift-diffusion modeling of perovskite cells
混合钙钛矿的介电特性和钙钛矿细胞的漂移扩散建模
- DOI:
- 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
L. Pédesseau;M. Képénekian;D. Sapori;Yong Huang;A. Rolland;Alexandre Beck;C. Cornet;O. Durand;Shijian Wang;C. Katan;J. Even - 通讯作者:
J. Even
Does aggressiveness help? Evidence from IPO corruption and pricing in China
攻击性有帮助吗?
- DOI:
10.1016/j.jcorpfin.2021.101901 - 发表时间:
2021-04 - 期刊:
- 影响因子:6.1
- 作者:
Yong Huang;Chao Yan;Kam C. Chan - 通讯作者:
Kam C. Chan
Yong Huang的其他文献
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{{ truncateString('Yong Huang', 18)}}的其他基金
Pore Formation and Polymer Thermal Debinding during Vapor-Induced Phase Separation-Enabled Metal Printing
蒸汽诱导相分离金属打印过程中的孔形成和聚合物热脱脂
- 批准号:
2315811 - 财政年份:2023
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Manufacturing USA: Study of Self-Supporting Nanoclay as Internal Scaffold Material for Printing of Skeletal Tissue Constructs
美国制造:自支撑纳米粘土作为骨骼组织结构打印内部支架材料的研究
- 批准号:
1762941 - 财政年份:2018
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
GOALI: Printing of Heterogeneous Tissue Constructs from Reactive Biomaterials using Intersecting Jets
GOALI:使用相交喷射机打印反应性生物材料的异质组织结构
- 批准号:
1634755 - 财政年份:2016
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Scalable Laser Printing of Three-Dimensional Living Tissue Constructs
三维活组织结构的可扩展激光打印
- 批准号:
1537956 - 财政年份:2015
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Workshop: Environmental Implications of Additive Manufacturing; Arlington, Virginia; October 14-15, 2014
研讨会:增材制造的环境影响;
- 批准号:
1450529 - 财政年份:2014
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Collaborative Research: Understanding Machining-Induced Influences to Ultra-Fine Grained Pure Titanium for Biomedical Applications
合作研究:了解机械加工对生物医学应用超细晶纯钛的影响
- 批准号:
1404926 - 财政年份:2014
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
CAREER: Understanding Process-Induced Damage in Laser-Assisted Cell Direct Writing - Bridging Manufacturing Science and Biomedical Research
职业:了解激光辅助细胞直写过程中引起的损伤 - 连接制造科学和生物医学研究
- 批准号:
1321271 - 财政年份:2013
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
NSF Workshop on Frontiers of Additive Manufacturing Research and Education; Arlington, Virginia; 11-12 July 2013
NSF 增材制造研究和教育前沿研讨会;
- 批准号:
1339027 - 财政年份:2013
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Fabrication of Double-Layer Cellular Spheroid using Acoustic Excitation-Assisted Compound Jetting
使用声激励辅助复合喷射制备双层细胞球体
- 批准号:
1314834 - 财政年份:2013
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
Collaborative Research: Laser-Assisted Orifice-Free Fabrication of Viscous Alginate Microspheres
合作研究:激光辅助无孔制造粘性海藻酸盐微球
- 批准号:
1314830 - 财政年份:2013
- 资助金额:
$ 30万 - 项目类别:
Standard Grant
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