Profiling Translated mRNAs from the Organ to Specific Cell Level for Modeling Mechanisms of Gene Regulation and Response to Hypoxia

分析从器官到特定细胞水平的翻译 mRNA，以模拟基因调控和缺氧反应的机制

• 批准号: 0750811
• 负责人: Julia Bailey-Serres
• 金额: $ 54.31万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0750811&HistoricalAwards=false
• 关键词: Profiling; Translated; mRNAs; Organ; Specific

Bailey-Serres Proposal # IOS-0750811Profiling Translated mRNAs from the Organ to Specific Cell Level for Modeling Mechanisms of Gene Regulation and Response to HypoxiaPlants and animals use oxygen from the atmosphere to produce the energy needed for cell function. These organisms respond in an exquisite manner when oxygen levels are diminished. This can occur in plant roots when a field is flooded or in a brain cell during a heart attack or stroke. The goal is to understand the process by which cells endure transient periods of inadequate oxygen. The focus is on the conservation of cellular energy that occurs through a change in the manner in which proteins are synthesized from gene transcripts - the intermediate between gene and protein. In plants, as well as in animals, cells that lack oxygen become more selective in protein synthesis. This occurs by storing most gene transcripts. However, some gene transcripts escape this repression; these generally encode the proteins that the cell requires to cope with the stress. This project will study the regulation of gene transcript use during oxygen deprivation. This will include evaluation of the management in the root and the shoot, as well as in specific layers of cells. This analysis will provide a level of resolution that has previously not been obtained - yielding information on the response to the stress that occurs in organs and specialized cells. The research will also address the mechanism by which cells store gene transcripts, in order to spare energy. A broader impact will be cross-disciplinary training of scientists: a biologist, will be trained in computer science and a chemist will be trained in plant physiology. Undergraduate students, some of whom will be from small colleges, will gain hands-on research experience. Another broader impact will be the provision of plant lines to the research community that can be used to monitor gene transcripts associated with the protein synthesis machinery in specialized cell types.

Bailey-Serres提案#IOS-0750811分析从器官到特定细胞水平的mRNAs，用于模拟基因调控和对低氧的反应植物和动物利用大气中的氧气来产生细胞功能所需的能量。当氧气水平降低时，这些有机体会以一种巧妙的方式做出反应。这可能发生在农田被淹时的植物根部，或者在心脏病发作或中风期间的脑细胞中。其目标是了解细胞耐受氧气不足的短暂时期的过程。重点是通过改变基因转录物合成蛋白质的方式来守恒细胞能量--基因转录物是基因和蛋白质之间的中间产物。在植物和动物中，缺氧的细胞在蛋白质合成方面变得更具选择性。这是通过存储大多数基因转录本来实现的。然而，一些基因转录本逃脱了这种抑制；这些转录本通常编码细胞应对压力所需的蛋白质。本项目将研究缺氧过程中基因转录物的使用规律。这将包括对根部和地上部以及特定细胞层的管理进行评估。这项分析将提供以前从未获得过的分辨率水平--产生关于器官和特殊细胞对应激反应的信息。这项研究还将探讨细胞储存基因转录本的机制，以节省能量。更广泛的影响将是科学家的跨学科培训：一名生物学家将接受计算机科学培训，一名化学家将接受植物生理学培训。本科生，其中一些将来自小型学院，将获得实践研究经验。另一个更广泛的影响将是向研究界提供植物品系，这些植物品系可用于监测与特定细胞类型的蛋白质合成机制相关的基因转录本。