MRI: Acquisition of a Microarray Scanner and Real-Time PCR System for Interdisciplinary Research and Teaching in an Undergraduate College Setting

MRI：采购微阵列扫描仪和实时 PCR 系统，用于本科院校的跨学科研究和教学

• 批准号: 0821057
• 负责人: Margaret Kovach
• 金额: $ 8.73万
• 依托单位: University of Tennessee Chattanooga
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0821057&HistoricalAwards=false
• 关键词: MRI; Acquisition; Microarray; Scanner; Real

A grant has been awarded to the University of Tennessee at Chattanooga under the direction of Margaret J. Kovach to acquire a microarray scanner and real-time PCR system to enhance undergraduate research and education in molecular biology. The instrumentation will be used by faculty and students in the departments of Biological and Environmental Sciences and Chemistry to elucidate changes in gene expression associated with biological processes. Microarray analysis offers the advantage of profiling expression levels of thousands of genes simultaneously, whereas real-time PCR analysis provides precise quantification of expression levels for a small set of genes. In this proposal, these methods will be applied to model systems to measure responses to experimental stimuli to gain a better understanding of the molecular events behind certain biological processes. Specifically, this proposal is comprised of 13 research and teaching activities by five faculty members that represent a wide range of biological research and organismal systems including: mechanisms of gene regulation associated with genomic instability and tissue differentiation in mammals, physiological stress responses in plants, muscle and bone development in fish, the role of biologically active small molecules on biochemical processes and the activity of soil microbes in bioremediation and biogeochemistry of forests and wetlands. This proposal will enhance faculty development and student growth by enriching course offerings in molecular biology, plant physiology, developmental biology, biochemistry and microbiology through integration of hands-on exposure to advanced genomic and proteomic applications such as microarray analysis and quantitative real-time PCR. Pivotal to the student?s education is increasing the number and variety of experimental procedures available for independent research projects through modern instrumentation. Ultimately, this grant will serve to train young scientists in cutting edge technologies relevant to post-graduate studies and employment in molecular biology and biotechnology.

在Margaret J. Kovach的指导下，田纳西州查塔努加大学获得了一笔赠款，用于购买微阵列扫描仪和实时PCR系统，以加强分子生物学的本科生研究和教育。该仪器将被生物与环境科学和化学系的教师和学生使用，以阐明与生物过程相关的基因表达变化。微阵列分析提供了同时分析数千个基因表达水平的优势，而实时PCR分析提供了一小部分基因表达水平的精确定量。在本提案中，这些方法将应用于模型系统，以测量对实验刺激的反应，以更好地理解某些生物过程背后的分子事件。具体来说，该提案由5名教师的13项研究和教学活动组成，这些活动代表了广泛的生物研究和有机系统，包括：与基因组不稳定性和哺乳动物组织分化相关的基因调控机制，植物生理应激反应，鱼类肌肉和骨骼发育，生物活性小分子在生物化学过程中的作用以及森林和湿地生物修复和生物地球化学中土壤微生物的活性。该提案将通过整合先进的基因组学和蛋白质组学应用（如微阵列分析和实时定量PCR），丰富分子生物学、植物生理学、发育生物学、生物化学和微生物学方面的课程，促进教师的发展和学生的成长。对学生至关重要吗？美国的教育正在通过现代仪器为独立研究项目增加实验程序的数量和种类。最终，这笔拨款将用于培训年轻科学家，使他们掌握与分子生物学和生物技术的研究生学习和就业相关的尖端技术。