Controlling Microscale Collagen Peptide Assembly with Metal Triggers

用金属触发器控制微型胶原蛋白肽组装

• 批准号: 0848325
• 负责人: Jean Chmielewski
• 金额: $ 50.1万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0848325&HistoricalAwards=false
• 关键词: Controlling; Microscale; Collagen; Peptide; Assembly

Controlling Microscale Collagen Peptide Self Assembly with Metal TriggersCollagen is one of the major structural proteins of the extracellular matrix and is found associated with skin, bone, tendons, ligaments, and blood vessels. As such, there is great interest in using collagen for applications in regenerative medicine and tissue engineering. Natural collagen has limitations, however, for these applications due to difficulties in precisely controlling the protein's structure at the nano- and micro-scale and a limited ability to modify its chemical structure. These difficulties could be overcome by using synthetic fragments of collagen (peptides) as building blocks for larger, functional biomaterials. To this end, the Chmielewski group has designed collagen peptide building blocks that can be assembled using metal ion/ligand interactions to generate unique micron-scaled structures, such as fibers, spheres, meshes and disks.The overall goals of the proposed research are to understand the mechanism used in the formation of the collagen peptide assemblies and to fully optimize the conditions needed to generate distinct collagen peptide microstructures on demand. Specific questions that will be addressed include: (1) What roles do the metal-ligand combination and the chemical structure of the collagen building block play in the assembly of nano- to micro-scaled structures? (2) What is the mechanism of the formation of the collagen peptide microstructures? (3) Can features of different building block design strategies be combined to create modular microstructures?The initial designs of collagen peptide assembly using metal-ligand interactions have provided fascinating microstructures, some of unprecedented sizes and shape. The intellectual merit of the proposed studies is focused on gaining a firm understanding of the parameters and mechanisms of the assembly process. The metal-promoted assembly of collagen peptides into micro-scale materials is evocative and may have broad impact on many areas of science, including bio-nanotechnology, tissue engineering and drug delivery. The results of the proposed scientific questions will, therefore, have a broad impact on society, as will the training of the students who participate in this interdisciplinary area of research.

胶原蛋白是细胞外基质的主要结构蛋白之一，被发现与皮肤、骨骼、肌腱、韧带和血管有关。 因此，人们对将胶原蛋白用于再生医学和组织工程中的应用非常感兴趣。 然而，天然胶原蛋白对于这些应用具有局限性，这是由于难以在纳米和微米尺度上精确控制蛋白质的结构以及改变其化学结构的能力有限。 这些困难可以通过使用胶原蛋白（肽）的合成片段作为更大的功能性生物材料的构建块来克服。 为此，Chmielewski小组设计了胶原蛋白肽构建模块，可以使用金属离子/配体相互作用组装，以产生独特的微米级结构，如纤维，球体，所提出的研究的总体目标是了解在胶原蛋白肽组装体的形成中使用的机制，并充分优化产生不同的胶原蛋白肽微结构所需的条件。需求 具体的问题将被解决包括：（1）什么样的角色做的金属配体组合和胶原蛋白构建块的化学结构在组装纳米到微米尺度的结构？ (2)胶原肽微结构的形成机制是什么？ (3)不同积木式设计策略的特征能否结合起来创建模块化微结构？使用金属-配体相互作用的胶原蛋白肽组装的初始设计提供了迷人的微观结构，其中一些具有前所未有的尺寸和形状。 拟议研究的智力价值集中在获得对组装过程的参数和机制的深刻理解上。 金属促进的胶原肽组装成微米级材料是令人回味的，并且可能对许多科学领域产生广泛影响，包括生物纳米技术、组织工程和药物递送。 因此，所提出的科学问题的结果将对社会产生广泛的影响，参与这一跨学科研究领域的学生的培训也将如此。