MRI: Acquisition of a multiphoton confocal microscope for research, training and teaching

MRI：购买多光子共焦显微镜用于研究、培训和教学

• 批准号: 0923559
• 负责人: Sean Wilson
• 金额: $ 101.8万
• 依托单位: Loma Linda University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923559&HistoricalAwards=false
• 关键词: MRI; Acquisition; multiphoton; confocal; microscope

This award for the acquisition of a multiphoton laser scanning confocal microscope has established a shared-use imaging facility that will serve varied and extensive research and training purposes for basic scientists at Loma Linda University. This advanced microscopy system will provide a broad range of imaging technologies including multiphoton and spectral analysis, as well as real-time imaging. The system is equipped with UV and multiple lasers for visible and multiphoton excitation, a multi-channel scanning module for multispectral detection and point scanning, a transmitted-light detector, and 2 non-descanned detectors for multiphoton imaging. This array of excitation and emission detection systems will provide researchers the capacity to use multiple fluorophores for innovative multidimensional imaging studies. To promote investigation of basic mechanisms of cellular interactions and function and perform live-cell and tissue studies, the system will include an incubation stage, an electrophysiology system, and myograph apparatus. The confocal microscope will be integrated into a microscopy training program, academic courses, and training workshops. The visual nature of the images acquired by the proposed system make an excellent entry point for students to engage in research. Students will be recruited through established outreach and research internship and graduate programs at Loma Linda University that target high-school age, undergraduate and graduate students and post-graduate trainees with a strong emphasis on under-represented minorities. Our goal for use of the proposed instrumentation is to facilitate multidisciplinary research and training at Loma Linda University and in the surrounding community. This facility and its members will provide the resource base and essential infrastructure that meet the needs for enduring collaboration and education, as well as serve as a catalyst that promotes basic science productivity in a diverse academic community.

该奖项用于收购多光子激光扫描共聚焦显微镜，建立了一个共享使用的成像设施，将为洛马琳达大学的基础科学家提供各种广泛的研究和培训。这种先进的显微镜系统将提供广泛的成像技术，包括多光子和光谱分析，以及实时成像。该系统配备了用于可见光和多光子激发的UV和多个激光器，用于多光谱检测和点扫描的多通道扫描模块，透射光检测器和用于多光子成像的2个非去扫描检测器。这种激发和发射检测系统阵列将为研究人员提供使用多种荧光团进行创新多维成像研究的能力。为了促进对细胞相互作用和功能的基本机制的研究，并进行活细胞和组织研究，该系统将包括孵育阶段、电生理系统和肌电描记仪。共焦显微镜将被整合到显微镜培训计划，学术课程和培训研讨会。所提出的系统获得的图像的视觉性质，使学生从事研究的一个很好的切入点。将通过洛马琳达大学既定的外联和研究实习及研究生方案招募学生，这些方案的目标是高中年龄、本科生和研究生以及研究生受训人员，重点是代表性不足的少数群体。我们的目标是使用拟议的仪器，以促进多学科的研究和培训在洛马琳达大学和周围社区。该设施及其成员将提供资源基础和必要的基础设施，以满足持久合作和教育的需求，并作为促进基础科学生产力在一个多元化的学术界的催化剂。