IDR: ECCS - EPDT: Ultra high-throughput holographic on-chip cytometry using inertial micro-fluidics

“该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。流式细胞术是现代细胞生物学和临床诊断的主力，但具有三个显著的缺点：（i）有限的通量;（ii）高成本;和（iii）在小型化的同时仍然保持其通量的挑战。解决这些局限性可能会增加细胞仪的可用性和应用领域，显著改变生物医学研究和临床分析。在该提议中，为了解决细胞分析平台的现有限制，目的是（1）将惯性微流体聚焦与片上全息成像平台集成;以及（2）测试该集成系统用于利用白色血细胞差异进行全血细胞计数的性能。鉴于其紧凑且具有成本效益的设计，没有鞘液或透镜，加上其超大吞吐量和独特的全息信息内容，所提出的片上细胞仪平台有望对干细胞，免疫学癌症相关研究产生变革性影响&，以及临床诊断，特别是在资源有限或该提案还将建立一个补充性的教育推广计划，该计划将涉及来自加利福尼亚州圣费尔南多谷主要贫困和社会经济影响社区的高中学生。暑期研究项目，研讨会和开放参观将为高中生提供与前沿研究环境互动的服务，有助于提高他们的科学好奇心，并塑造他们在科学和工程领域的职业目标。此外，在本提案的时间轴内，为了更好地促进UCLA校园内代表性不足的工程专业学生的发展、招聘、保留和毕业，将创造几个本科生研究机会，以补充PI在这方面的现有活动。最后，一个新的研究生水平的课程，题为“实验室上的一个芯片光子学”，将开发，其目的是整合“知识”;和“设计”;工程教育方面到一个单一的课程。

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."0930501OzcanFlow cytometry is a workhorse of modern cell biology and clinical diagnostics, but suffers from three significant downsides: (i) limited throughput; (ii) high cost; and (iii) challenges towards miniaturization while still keeping its throughput. Addressing these limitations is likely to increase the availability and application areas of cytometers, significantly transforming biomedical research and clinical analysis. In this proposal, to address the existing limitations of cell analysis platforms, the aim is to (1) Integrate inertial microfluidic focusing with an on-chip holographic imaging platform; and (2) Test the performance of this integrated system for conducting complete blood counts with white blood cell differentials. Given its compact and cost-effective design without sheath fluids or lenses, together with its ultra-large throughput, and unique holographic information content, the proposed on-chip cytometry platform is poised to have a transformative impact for stem cell, immunology & cancer related research, as well as clinical diagnostics especially in resource limited or point-of-care settings.This proposal will also establish a complementary educational outreach program which will involve high school students from a predominantly underprivileged and socio-economically impacted neighborhood of San Fernando Valley, CA. Summer research projects, seminars and open house visits will serve the high school students to interact with a cutting edge research environment, helping to increase their scientific curiosity and shaping their career goals in science and engineering. Furthermore, during the timeline of this proposal, to better contribute to the development, recruitment, retention, and graduation of underrepresented engineering students on UCLA campus, several undergraduate research opportunities will be created to supplement the existing activities of the PI's in this regard. Finally, a new graduate level course titled "Lab-on-a-Chip Photonics"; will be developed, which aims to integrate "knowledge"; and "design"; aspects of engineering education into a single course.