IDR: ECCS - EPDT: Ultra high-throughput holographic on-chip cytometry using inertial micro-fluidics

“该奖项是根据 2009 年美国复苏和再投资法案（公法 111-5）提供资金的。”0930501Ozcan 流式细胞术是现代细胞生物学和临床诊断的主力，但存在三个显着的缺点：(i) 通量有限； (二)成本高； (iii) 在保持吞吐量的同时实现小型化的挑战。解决这些限制可能会增加细胞仪的可用性和应用领域，从而显着改变生物医学研究和临床分析。在该提案中，为了解决细胞分析平台现有的局限性，目标是（1）将惯性微流控聚焦与片上全息成像平台集成； (2) 测试该集成系统进行全血细胞计数和白细胞分类的性能。鉴于其紧凑且经济高效的设计，无需鞘液或透镜，加上其超大吞吐量和独特的全息信息内容，拟议的片上细胞计数平台有望对干细胞、免疫学和癌症相关研究以及临床诊断产生变革性影响，特别是在资源有限或定点护理环境中。该提案还将建立一个补充性教育推广计划，该计划将涉及来自圣费尔南多谷主要贫困和社会经济影响社区的高中生，加利福尼亚州。夏季研究项目、研讨会和开放日参观将为高中生提供与前沿研究环境互动的机会，帮助提高他们的科学好奇心并塑造他们在科学和工程领域的职业目标。此外，在该提案期间，为了更好地促进加州大学洛杉矶分校校园中代表性不足的工程专业学生的发展、招聘、保留和毕业，将创建一些本科生研究机会，以补充 PI 在这方面的现有活动。最后，一门新的研究生课程，名为“芯片实验室光子学”；将被开发，其目的是整合“知识”；和“设计”；将工程教育的各个方面整合到一门课程中。

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."0930501OzcanFlow cytometry is a workhorse of modern cell biology and clinical diagnostics, but suffers from three significant downsides: (i) limited throughput; (ii) high cost; and (iii) challenges towards miniaturization while still keeping its throughput. Addressing these limitations is likely to increase the availability and application areas of cytometers, significantly transforming biomedical research and clinical analysis. In this proposal, to address the existing limitations of cell analysis platforms, the aim is to (1) Integrate inertial microfluidic focusing with an on-chip holographic imaging platform; and (2) Test the performance of this integrated system for conducting complete blood counts with white blood cell differentials. Given its compact and cost-effective design without sheath fluids or lenses, together with its ultra-large throughput, and unique holographic information content, the proposed on-chip cytometry platform is poised to have a transformative impact for stem cell, immunology & cancer related research, as well as clinical diagnostics especially in resource limited or point-of-care settings.This proposal will also establish a complementary educational outreach program which will involve high school students from a predominantly underprivileged and socio-economically impacted neighborhood of San Fernando Valley, CA. Summer research projects, seminars and open house visits will serve the high school students to interact with a cutting edge research environment, helping to increase their scientific curiosity and shaping their career goals in science and engineering. Furthermore, during the timeline of this proposal, to better contribute to the development, recruitment, retention, and graduation of underrepresented engineering students on UCLA campus, several undergraduate research opportunities will be created to supplement the existing activities of the PI's in this regard. Finally, a new graduate level course titled "Lab-on-a-Chip Photonics"; will be developed, which aims to integrate "knowledge"; and "design"; aspects of engineering education into a single course.