IDR: ECCS - EPDT: Ultra high-throughput holographic on-chip cytometry using inertial micro-fluidics

IDR：ECCS - EPDT：使用惯性微流体的超高通量全息片上细胞术

• 批准号: 0930501
• 负责人: Aydogan Ozcan
• 金额: $ 59.88万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0930501&HistoricalAwards=false
• 关键词: IDR; ECCS; EPDT; Ultra; throughput

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."0930501OzcanFlow cytometry is a workhorse of modern cell biology and clinical diagnostics, but suffers from three significant downsides: (i) limited throughput; (ii) high cost; and (iii) challenges towards miniaturization while still keeping its throughput. Addressing these limitations is likely to increase the availability and application areas of cytometers, significantly transforming biomedical research and clinical analysis. In this proposal, to address the existing limitations of cell analysis platforms, the aim is to (1) Integrate inertial microfluidic focusing with an on-chip holographic imaging platform; and (2) Test the performance of this integrated system for conducting complete blood counts with white blood cell differentials. Given its compact and cost-effective design without sheath fluids or lenses, together with its ultra-large throughput, and unique holographic information content, the proposed on-chip cytometry platform is poised to have a transformative impact for stem cell, immunology & cancer related research, as well as clinical diagnostics especially in resource limited or point-of-care settings.This proposal will also establish a complementary educational outreach program which will involve high school students from a predominantly underprivileged and socio-economically impacted neighborhood of San Fernando Valley, CA. Summer research projects, seminars and open house visits will serve the high school students to interact with a cutting edge research environment, helping to increase their scientific curiosity and shaping their career goals in science and engineering. Furthermore, during the timeline of this proposal, to better contribute to the development, recruitment, retention, and graduation of underrepresented engineering students on UCLA campus, several undergraduate research opportunities will be created to supplement the existing activities of the PI's in this regard. Finally, a new graduate level course titled "Lab-on-a-Chip Photonics"; will be developed, which aims to integrate "knowledge"; and "design"; aspects of engineering education into a single course.

“这项奖励是根据2009年美国复苏和再投资法案（公法111-5）资助的。”ozcanflow cytometry（流式细胞术）是现代细胞生物学和临床诊断的主力，但有三个显著的缺点：(i)有限的吞吐量；（二）成本高；（三）在保持其吞吐量的同时实现小型化的挑战。解决这些限制可能会增加细胞仪的可用性和应用领域，显著改变生物医学研究和临床分析。为了解决现有细胞分析平台的局限性，本文的目标是：(1)将惯性微流控聚焦与片上全息成像平台集成；(2)测试该综合系统的性能，以进行白细胞分化的全血细胞计数。鉴于其紧凑且经济高效的设计，无需鞘液或透镜，加上其超大吞吐量和独特的全息信息内容，拟议的片上细胞仪平台将对干细胞，免疫学和癌症相关研究以及临床诊断产生变革性影响，特别是在资源有限或护理点环境中。该提案还将建立一个补充的教育推广计划，该计划将涉及来自加利福尼亚州圣费尔南多谷主要贫困和社会经济受影响社区的高中生。夏季研究项目，研讨会和开放参观将为高中生提供与前沿研究环境的互动，帮助提高他们的科学好奇心并塑造他们在科学和工程方面的职业目标。此外，在本提案实施期间，为了更好地促进加州大学洛杉矶分校校园内未被充分代表的工程学生的发展、招聘、保留和毕业，将创造几个本科生研究机会，以补充PI在这方面的现有活动。最后，一个名为“芯片实验室光子学”的新研究生水平课程；将开发，其目的是整合“知识”；和“设计”;将工程教育的各个方面纳入单一课程。