EAGER: Carbide Derived Carbons with Pore Structure and Surface Chemistry Designed for Selective Adsorption of Proteins

EAGER：具有孔结构和表面化学性质的碳化物衍生碳，专为选择性吸附蛋白质而设计

• 批准号: 0945230
• 负责人: Yury Gogotsi
• 金额: $ 12.5万
• 依托单位: Drexel University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0945230&HistoricalAwards=false
• 关键词: EAGER; Carbide; Derived; Carbons; Pore

NON-TECHNICAL DESCRIPTION: Severe sepsis kills about the same number of people per day as lung or breast cancer. Since the inflammatory response in case of sepsis is driven by cytokines, their removal from blood brings under control the unregulated pro- and anti-inflammatory processes driving sepsis. Unlike dialysis and filtration, not to mention drug-based therapy, adsorption can remove cytokines from blood plasma without introducing any other substances into the blood. The proposed research is expected to provide a tool for better understanding of the fundamental mechanisms governing the adsorption of cytokines by carbon materials. The use of the results of this study in medicine may save the lives of people suffering from autoimmune diseases, severe sepsis, and multiple organ failure. This program will facilitate an interdisciplinary international cooperation between the research groups working on nanoporous carbons in Philadelphia (U.S.) and Brighton (U.K.). Exchange with the group of Professor S. Mikhalovsky, University of Brighton, will allow graduate and undergraduate students from Drexel University to obtain international experience. TECHNICAL DETAILS: The goal of this research is to investigate the effect of pore size, pore shape, particle size, and surface termination of carbide-derived carbons (CDC) on adsorption of cytokines and other proteins. In the CDC process, the metal is extracted from a binary or ternary carbide or carbonitride ceramic by halogens. Therefore, the structure of the carbon can be templated by the carbide structure, with an opportunity for further tuning by controlling the temperature and composition of the environment. Preliminary testing at the University of Brighton has demonstrated that CDC samples significantly outperform the best commercial carbons in the efficiency of protein adsorption. This important breakthrough warrants conducting further studies immediately to explore if a truly transformational approach to sepsis can be carried out. Experimental studies will be performed on cytokine (interleukins and TNF-alpha) adsorption kinetics as a function of the pore size (5-50 nm) by using CDC produced from different precursors. The effects of surface functional groups on the interaction of proteins with the carbon will be experimentally investigated. The PI will determine the conditions for selective adsorption of specific proteins and develop materials with the surface termination and pore size tuned to maximize adsorption capacity and kinetics of adsorption for selected proteins.

非技术描述：严重脓毒症每天杀死的人数与肺癌或乳腺癌相同。 由于脓毒症情况下的炎症反应是由细胞因子驱动的，因此从血液中去除它们会控制驱动脓毒症的不受调节的促炎和抗炎过程。与透析和过滤不同，更不用说基于药物的治疗，吸附可以从血浆中去除细胞因子，而不会将任何其他物质引入血液中。该研究有望为更好地理解碳材料吸附细胞因子的基本机制提供工具。这项研究的结果在医学上的使用可能会挽救患有自身免疫性疾病，严重败血症和多器官衰竭的人的生命。 该计划将促进费城（美国）纳米多孔碳研究小组之间的跨学科国际合作。布莱顿（英国）与S教授小组交流。布莱顿大学的Mikhalovsky将允许德雷克塞尔大学的研究生和本科生获得国际经验。技术规格：本研究的目的是研究碳化物衍生碳（CDC）的孔径，孔形状，颗粒大小和表面终止对细胞因子和其他蛋白质吸附的影响。在CDC工艺中，金属通过卤素从二元或三元碳化物或碳氮化物陶瓷中提取。因此，碳的结构可以通过碳化物结构来模板化，并有机会通过控制环境的温度和组成来进一步调整。布莱顿大学的初步测试表明，CDC样品在蛋白质吸附效率方面明显优于最好的商业碳。这一重要的突破值得立即进行进一步的研究，以探索是否可以对脓毒症进行真正的变革性方法。将通过使用由不同前体产生的CDC对作为孔径（5-50 nm）的函数的细胞因子（白介素和TNF-α）吸附动力学进行实验研究。将实验研究表面官能团对蛋白质与碳相互作用的影响。PI将确定选择性吸附特定蛋白质的条件，并开发具有表面终止和孔径调整的材料，以最大限度地提高选定蛋白质的吸附能力和吸附动力学。