RAPID: ACQUISITION OF A PROTEOMICS ANALYZER TO ELUCIDATE PATHWAYS OF PETROLEUM HYDROCARBON BIOREMEDIATION IN THE GULF OF MEXICO

RAPID：购买蛋白质组分析仪来阐明墨西哥湾石油碳氢化合物生物修复的途径

• 批准号: 1057414
• 负责人: Kartik Chandran
• 金额: $ 20万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1057414&HistoricalAwards=false
• 关键词: RAPID; ACQUISITION; PROTEOMICS; ANALYZER; ELUCIDATE

PI: Kartik ChandranProposal Number: 1057414Institution: Columbia UniversityTitle: OIA: Acquisition of a Proteomics Analyzer to Elucidate Pathways of Petroleum Hydrocarbon Bioremediation in the Gulf of MexicoThis NSF MRI RAPID project will support the acquisition of a state-of-the art instrument to elucidate the microbial mechanisms of methane and complex petroleum hydrocarbon biodegradation at the quantitative proteomics level. The mechanistic information thus obtained will be used to develop bioremediation strategies to alleviate the environmental insults that have resulted from the Deepwater Horizon oil spill in the Gulf of Mexico. The Deepwater Horizon oil spill has resulted in drastically impaired aquatic life and environmental health of the gulf. Nevertheless, the specific chemical composition of the contaminant stream presents a novel opportunity for simultaneous bioremediation of both the primary organics (the petroleum hydrocarbons) and the methane (the purported cause of the explosion that led to the spill). Methane is typically the primary energy substrate for methane oxidizing bacteria (MOB). However, owing to the broad substrate specificity of the first enzyme involved in methane oxidation to formaldehyde (methane monooxygenase), MOB can oxidize not only methane but also alternate organic substrates such as longer chain aliphatic and aromatic compounds. However, MOB cannot derive energy from such transformations, which are termed ?co-metabolic?. Co-metabolism based bioremediation strategies are therefore characterized by a finite transformation capacity, limited by availability of the primary substrate (in casu, methane). In this project, they will elucidate the proteomics scale mechanisms of MOB to oxidize methane (via primary energy metabolism) and the mix of petroleum hydrocarbons (via co-metabolism) in lab-scale bioreactors. This NSF MRI Rapid project will support the acquisition of a quantitative proteomics analyzer (Waters Corp.), which will be housed initially for six-months in Earth and Environmental Engineering (the home department of the PI, Dr. Kartik Chandran) and then deployed onsite in the Gulf. When used for ex-situ bioremediation of the sequestered or skimmed pollutants along with the methane (which is currently just being flared or released), the bio-process technology developed in this project could be specifically applied to address both contaminants simultaneously. Mathematical models that describe methane oxidation and co-metabolism of petroleum hydrocarbons will be constructed and parameterized using the proteomics data combined with chemical profiles and microbial measurements. Finally, operating strategies, ex-situ transformation and mineralization of methane and petroleum hydrocarbons mixes obtained on site will be developed and tested. Successful application of this project will enable the accelerated biological treatment of the widespread petroleum hydrocarbon and methane pollution in the Gulf of Mexico. Additionally, the developed strategies will help to accelerate treatment and minimize such widespread dissemination of this particular contaminant mix, which is typical of offshore drilling operations in the future.This instrument will be used for Gulf oil spill related research in a timely fashion consistent with RAPID funding requirements.

PI：Kartik Chandran 提案编号：1057414 机构：哥伦比亚大学 标题：OIA：采购蛋白质组分析仪以阐明墨西哥湾石油碳氢化合物生物修复的途径 该 NSF MRI RAPID 项目将支持采购最先进的仪器，以阐明甲烷和复杂石油的微生物机制 定量蛋白质组学水平上的碳氢化合物生物降解。由此获得的机械信息将用于制定生物修复策略，以减轻墨西哥湾深水地平线漏油事件造成的环境污染。深水地平线漏油事件严重损害了海湾的水生生物和环境健康。然而，污染物流的特定化学成分为同时对主要有机物（石油烃）和甲烷（据称导致泄漏的爆炸原因）进行生物修复提供了新的机会。甲烷通常是甲烷氧化细菌（MOB）的主要能源底物。然而，由于参与甲烷氧化成甲醛的第一种酶（甲烷单加氧酶）具有广泛的底物特异性，MOB 不仅可以氧化甲烷，还可以氧化其他有机底物，例如长链脂肪族和芳香族化合物。然而，MOB 无法从这种转化中获取能量，这种转化被称为“共代谢”。因此，基于共代谢的生物修复策略的特点是转化能力有限，受到主要底物（例如甲烷）的可用性的限制。在这个项目中，他们将阐明 MOB 在实验室规模生物反应器中氧化甲烷（通过初级能量代谢）和石油烃混合物（通过共代谢）的蛋白质组学规模机制。该 NSF MRI 快速项目将支持购买一台定量蛋白质组分析仪（沃特世公司），该分析仪最初将在地球与环境工程（PI Kartik Chandran 博士的所在部门）放置六个月，然后部署在海湾地区。 当用于对封存或脱脂污染物以及甲烷（目前刚刚燃烧或释放）进行异位生物修复时，该项目开发的生物工艺技术可以专门用于同时处理这两种污染物。将使用蛋白质组数据结合化学特征和微生物测量来构建和参数化描述甲烷氧化和石油烃共代谢的数学模型。最后，将开发和测试现场获得的甲烷和石油烃混合物的操作策略、异地转化和矿化。该项目的成功应用将加速墨西哥湾广泛的石油碳氢化合物和甲烷污染的生物处理。此外，所制定的策略将有助于加速处理并最大程度地减少这种特定污染物混合物的广泛传播，这是未来海上钻井作业的典型现象。该仪器将根据 RAPID 资金要求及时用于海湾漏油相关研究。