MRI: Acquisition of an Orbitrap Lumos Mass Spectrometer for Plant Proteomics and Lipidomics

MRI：购买 Orbitrap Lumos 质谱仪用于植物蛋白质组学和脂质组学

• 批准号: 1827534
• 负责人: Bradley Evans
• 金额: $ 95.35万
• 依托单位: Donald Danforth Plant Science Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-10-01 至 2022-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1827534&HistoricalAwards=false
• 关键词: MRI; Acquisition; Orbitrap; Lumos; Mass

An award is made to the Donald Danforth Plant Science Center in Saint Louis, Missouri to purchase a high resolution/accurate mass Orbitrap mass spectrometer for plant proteomics and lipidomics studies. The investment in mass spectrometry technology and method development at the Danforth Center benefits integrated studies of plant development and metabolism and provides guiding protocols for the greater scientific community. The projects supported by this new instrumentation aim to increase productivity of food and biofuel crops through a detailed understanding of how plants turn energy provided by the sun to biomass and how plants sense and coordinate their physiology in response to seasonal change using their internal biological timepiece known as the circadian clock. As part of the development of new technologies, the investigators will perform a special study with undergraduates from Harris-Stowe State University and other nearby undergraduate institutions' students to mine the large data stream anticipated to come from the new instrument for biological treasure. The students will get firsthand experience with the contemporary proteomics techniques that may open the door to further bioanalytical studies in their careers. This will add to the educational outreach taking place at the Danforth Center. Currently, the training of tomorrow's scientists occurs through an active NSF-REU program, recruitment of high school students that work in Danforth Center laboratories for classroom credit, participation in a regional program for high school students - STARS (Students and Teachers As Research Scientists), workshops for teachers and an interactive middle- and high school mentoring program that connects our scientists with nearby schools. These programs are essential to core Danforth Center mission goals to strengthen the plant science community.The long-term goal is to improve plant productivity that: i) proficiently feeds a growing world population, ii) provides sustainable feed-stock alternatives to petroleum and iii) supplies useful bioactive compounds; all designed to improve quality of life. Advanced mass spectrometry instrumentation is needed perform complex stable isotope labeling experiments (an alternative to radioactive isotope labeling) and for characterizing post-translational modifications on proteins that are present a very low level in cells. A major impediment to understanding biochemical function in plants is our incomplete characterization of metabolism and proteins, the cellular machines that it. The dynamism of metabolite and protein levels, including their post-translational modifications (PTMs), are the result of linked networks that establish plant phenotype. Measurement of these biomolecules is critical to assessing plant health and response to changing environments; however, there are hundreds of thousands of metabolites found in the plant kingdom and organisms have more than a million of proteoforms that vary in abundance by many orders of magnitude and that change dynamically with time. Research efforts on NSF-funded and other projects at the Donald Danforth Plant Science Center and surrounding community are advancing science through methods development on scantly low abundance and highly dynamic proteins and metabolites and will be greatly extended by this technology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

位于密苏里州圣路易斯市的唐纳德·丹福斯植物科学中心被授予购买一台用于植物蛋白质组学和脂类组学研究的高分辨率/精确的质量轨道质谱仪。丹福斯中心对质谱学技术和方法开发的投资有利于对植物发育和新陈代谢的综合研究，并为更大的科学界提供指导方案。这一新仪器支持的项目旨在通过详细了解植物如何将太阳提供的能量转化为生物量，以及植物如何使用被称为生物钟的内部生物时钟来感知和协调它们的生理以应对季节性变化，从而提高粮食和生物燃料作物的生产率。作为新技术开发的一部分，研究人员将与哈里斯-斯托州立大学的本科生和附近其他本科生进行一项特别研究，以挖掘预计将来自新的生物宝藏仪器的大量数据流。学生们将获得当代蛋白质组学技术的第一手经验，这可能会为他们的职业生涯中进一步的生物分析研究打开大门。这将增加丹福斯中心正在进行的教育推广活动。目前，对未来科学家的培训是通过积极的NSF-REU计划、招募在丹福斯中心实验室工作的高中生获得课堂学分、参加面向高中生的地区性计划-STAR(学生和教师作为研究科学家)、教师研讨会以及将我们的科学家与附近学校联系起来的互动初中和高中导师计划来进行的。这些项目对于丹福斯中心加强植物科学界的核心使命目标至关重要。长期目标是提高植物生产率，即：i)熟练地养活不断增长的世界人口；ii)提供石油的可持续原料替代品；iii)提供有用的生物活性化合物；所有这些都旨在提高生活质量。需要先进的质谱仪进行复杂的稳定同位素标记实验(放射性同位素标记的替代方法)，以及对细胞中存在的非常低水平的蛋白质进行翻译后修饰的特征。理解植物生化功能的一个主要障碍是我们对新陈代谢和蛋白质的不完全描述，以及它所依赖的细胞机器。代谢产物和蛋白质水平的动态变化，包括它们的翻译后修饰(PTM)，是建立植物表型的连锁网络的结果。对这些生物分子的测量对于评估植物健康和对不断变化的环境的反应至关重要；然而，在植物界中发现了数十万种代谢物，生物体中有100多万种蛋白质形式，它们的丰度变化了许多个数量级，并且随着时间的变化而动态变化。唐纳德·丹福斯植物科学中心和周边社区对NSF资助的项目和其他项目的研究工作正在通过对低丰度和高活力蛋白质和代谢物的方法开发来推动科学，并将通过这项技术得到极大的扩展。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。