CAREER: Development of Microsecond Time-Resolved Mass Spectrometry for the Study of Biochemical Reaction Mechanisms and Kinetics

职业：开发微秒时间分辨质谱用于生化反应机制和动力学研究

• 批准号: 1149367
• 负责人: Hao Chen
• 金额: $ 57.48万
• 依托单位: Ohio University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1149367&HistoricalAwards=false
• 关键词: CAREER; Development; Microsecond; Time; Resolved

This CAREER award by the Chemical Measurement and Imaging (CMI) program of the Division of Chemistry supports work by Professor Hao Chen at Ohio University for the development of microsecond time-resolved mass spectrometry based on ionization of a flying liquid jet generated from ultrafast mixing of reactant solutions by an ambient ionization method. The proposed method is expected to improve sampling resolution from current millisecond sampling to micro-second time resolution. The sucessful development of the described technology will facilitate the investigation of kinetics and mechanisms of fast biochemical reactions, which may lead to a better understanding of protein folding/unfolding, enzymatic catalysis and protein/DNA oxidation processes.The work provides research opportunities for both undergraduate and graduate students, including members of underrepresented and disadvantaged groups. Dr. Chen is also actively involved in educational outreach activities, including ChemShows/Workshops/Seminars for pre-college students to learn about modern instrumental analysis and to develop enthusiasm for scientific research.

由化学学部化学测量与成像（CMI）项目颁发的CAREER奖支持俄亥俄大学陈浩教授的工作，他的工作是开发微秒时间分辨质谱法，该质谱法基于通过环境电离方法对由反应溶液的超快混合产生的飞行液体射流进行电离。该方法有望将采样分辨率从目前的毫秒级采样提高到微秒级采样。该技术的成功开发将有助于研究快速生化反应的动力学和机制，从而更好地理解蛋白质折叠/展开、酶催化和蛋白质/DNA氧化过程。这项工作为本科生和研究生提供了研究机会，包括代表性不足和弱势群体的成员。陈博士还积极参与教育推广活动，包括为大学预科学生提供ChemShows/ workshop /研讨会，以了解现代仪器分析并培养科学研究的热情。