CAREER: Plasmonic Nanoclusters with Built-in Artificial Antibodies for Label-free Biosensing

职业：具有内置人工抗体的等离激元纳米簇，用于无标记生物传感

• 批准号: 1254399
• 负责人: Srikanth Singamaneni
• 金额: $ 40万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1254399&HistoricalAwards=false
• 关键词: CAREER; Plasmonic; Nanoclusters; Built; Artificial

1254399 - SingamaneniPlasmonic sensors hold enormous potential for the development of low-cost, label-free, point-of-care biodiagnostics, rapid chemical detection and environmental monitoring. However, two major challenges need to be overcome to reap the benefits of this class of biosensors: (i) state-of-theart plasmonic biosensors either offer limited sensitivity or are impractical for real-world applications due to their poor stability and excessive cost; (ii) these biosensors rely on natural antibodies, which are high-cost and impose severe limitations in handling, storage and device integration. We propose to realize highly sensitive and inexpensive plasmonic sensors. The focus of the proposed effort will be hierarchically ordered, self-assembled arrays of chemically synthesized plasmonic nanostructures. We will incorporate artificial monoclonal antibodies and demonstrate these to be a highly sensitive, specific, stable and low-cost biodiagnostic platform. This will be achieved through orientation-controlled molecular imprinting.This proposal will lead to development of simpler and more robust biosensors for detection of multiple important compounds. This will be achieved by replacing antibodies - complicated, expensive and unstable molecules - with artificial equivalents based on less expensive and more stable materials. In addition, novel nanotechnology approaches will be used for detection.

1254399-Singamaneniplomonic传感器在开发低成本、无标签、护理点生物诊断、快速化学检测和环境监测方面具有巨大的潜力。然而，要获得这类生物传感器的好处，需要克服两个主要挑战：(I)最先进的等离子体生物传感器要么提供有限的灵敏度，要么由于其较差的稳定性和过高的成本而不切实际地应用于现实世界；(Ii)这些生物传感器依赖于天然抗体，后者成本高，并在处理、存储和设备集成方面造成严重限制。我们建议实现高灵敏度和低成本的等离子体传感器。拟议工作的重点将是化学合成的等离子体纳米结构的分级有序、自组装阵列。我们将加入人造单抗，并展示这些是一个高度敏感、特异、稳定和低成本的生物诊断平台。这将通过定向控制的分子印迹来实现。这一提议将导致开发出更简单、更强大的生物传感器来检测多种重要化合物。这将通过用基于更便宜、更稳定的材料的人造等价物取代抗体--复杂、昂贵和不稳定的分子--来实现。此外，还将使用新的纳米技术方法进行检测。