GOALI: 2D Optoelectronic Graphene Nanoprobes for Neural Network

1264982 -Xu本研究的目的是通过一个2D独立的光电石墨烯平台，研究从单突触到多神经元电路的亚微米到厘米尺寸尺度的信号处理原理。该方法是直接在大面积悬浮的石墨烯膜上生长神经元，这是液体环境中超灵敏石墨烯晶体管的一部分。通过石墨烯光电导测量、膜片钳记录和光学成像，研究神经元回路中轴突、树突和突触的电学、化学和光学变化。概述：神经元活动通常使用电极阵列刺激神经元细胞并记录其电响应。 该系统有几个限制：电极的数量通常很小，并且细胞相对于电极的放置没有很好地定义。 该提案将部署新材料石墨烯，用作培养神经元的表面。 石墨烯具有光电效应，这意味着可以使用显微镜光学监测细胞的电活动。 这将规避电极阵列的限制，并且将允许监测大量神经元细胞的活动。

1264982 - XuThe objective of this research is to investigate, across submicron to centimeter size scales, the principle of signal processing from single synapse to multi-neuron circuitry, via a 2D free-standing optoelectronic graphene platform. The approach is to grow neurons directly on a large-area suspended graphene membrane, which is part of an ultra-sensitive graphene transistor in a liquid environment. Through graphene photoconductance measuring, patch-clamp recording, and optical imaging, the electrical, chemical, and optical changes in axons, dendrites and synapses across a neuronal circuit will be investigated.General statement:Neuronal activity is typically mapped using electrode arrays that stimulate neuronal cells and record their electrical responses. This system has several limitations: the number of electrodes is typically small and placement of cells in relation to electrodes is not well defined. This proposal will deploy novel material, grapheme, to be used as the surface for culturing neurons. Grapheme has optoelectrical effect, meaning that electrical activity of cells may be monitored optically using a microscope. This would circumvent limitations of electrode arrays and would allow for monitoring activity of a much larger number of neuronal cells.