Investigating Membrane Proteins with Magnetic Resonance Spectroscopy

用磁共振波谱研究膜蛋白

• 批准号: 1305664
• 负责人: Gary Lorigan
• 金额: $ 43万
• 依托单位: Miami University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1305664&HistoricalAwards=false
• 关键词: Investigating; Membrane; Proteins; Magnetic; Resonance

This research award in the Chemistry of Life Processes (CLP) program supports work by Professor Gary A. Lorigan in the Department of Chemistry and Biochemistry at Miami University in Oxford, Ohio to probe the structure of membrane peptides using Electron Paramagnetic Resonance (EPR) spectroscopy. Membrane peptides are difficult to study because they are very hydrophobic. Thus, the peptides are incorporated into a lipid bilayer membrane. The objective of the proposed research is to develop state-of-the-art EPR spectroscopic techniques to probe the structural properties of integral membrane peptides. These techniques will be widely applicable to all peptide and protein systems. The pulsed EPR technique of ESEEM spectroscopy will be used to measure short-range distances (less than 8 Angstroms) between a spin label and deuterium nuclei on integral membrane peptides.It is expected that the ESEEM technique will become a valuable biophysical tool for measuring distances on chemical and biological systems. EPR spectroscopy is approximately a thousand fold more sensitive than NMR spectroscopy. This new biophysical technique can move the field forward by providing an efficient and easy way to distinguish between alpha-helical, 3-10-helical and beta-sheet structural segments. The potential future benefits of this basic research are to develop new techniques to obtain structural information on membrane proteins. This methodology is useful for all peptides and proteins, associated with energy shortages, electron transport, photosynthesis, and greenhouse gases. Through this research program undergraduate, graduate and postdoctoral students from diverse backgrounds are trained in advanced techniques in biophysical chemistry, helping to strengthen the US STEM workforce.

这项生命过程化学（CLP）项目的研究奖支持了俄亥俄州牛津市迈阿密大学化学和生物化学系Gary A. Lorigan教授利用电子顺磁共振（EPR）光谱探测膜肽结构的工作。由于膜肽具有很强的疏水性，因此很难对其进行研究。因此，多肽被纳入脂质双层膜。提出的研究目标是发展最先进的EPR光谱技术来探测整体膜肽的结构特性。这些技术将广泛适用于所有肽和蛋白质系统。ESEEM光谱的脉冲EPR技术将用于测量整体膜肽上自旋标记与氘核之间的短距离（小于8埃）。预计该技术将成为一种有价值的生物物理工具，用于测量化学和生物系统的距离。EPR光谱学的灵敏度大约是核磁共振光谱学的一千倍。这种新的生物物理技术通过提供一种有效和简单的方法来区分α -螺旋、3-10-螺旋和β -薄片结构片段，可以推动该领域的发展。这项基础研究的潜在未来效益是开发新的技术来获取膜蛋白的结构信息。该方法适用于所有与能源短缺、电子传递、光合作用和温室气体相关的肽和蛋白质。通过这一研究项目，来自不同背景的本科生、研究生和博士后接受了生物物理化学先进技术的培训，有助于加强美国的STEM劳动力。