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Investigating Membrane Proteins with Magnetic Resonance Spectroscopy

用磁共振波谱研究膜蛋白

基本信息

批准号：
1807131
负责人：
Gary Lorigan
金额：
$ 45万
依托单位：
Miami University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-01 至 2022-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1807131&HistoricalAwards=false
关键词：
Investigating Membrane Proteins Magnetic Resonance

项目摘要

With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Professor Gary A. Lorigan from the Department of Chemistry and Biochemistry at Miami University to investigate the structural and dynamic properties of a membrane protein known as pinholin. Novel powerful state-of-the-art techniques are used to study pinholin, which is an important membrane-bound protein that is directly involved in cell death. The last step of the virus infection cycle is cell death through lysis, the breakdown of the cell membrane. Professor Lorigan plan to develop the experimental procedures to characterize the structure and mechanism of the active and inactive forms of pinholin in a membrane. The pursuit allows graduate students, undergraduate students, and students from under-represented groups to obtain specialized training in cutting-edge Electron Paramagnetic Resonance (EPR) biophysical techniques. The new biophysical methods developed are useful generally to study a variety of different membrane protein systems. This research project also enables the development of a transformative STEM mentoring program called DUOS at Miami University that strengthens the synergy/interaction between graduate and undergraduate researchers to collaborate on a research project under the supervision of a faculty member in a Ph.D. granting department. One undergraduate/graduate student team from the Lorigan lab is scheduled to participate in this new program.The research project is conducted to characterize the structure, topology, dynamics, and oligomerization state of the active and inactive forms of pinholin. Powerful CW-EPR and pulsed EPR spectroscopic techniques are used to investigate the different formations of pinholin. Multiple distances are to be measured between spin labels to provide important structural information on the pinholin embedded in a membrane. Information from this study should provide new insight into the pinholin mechanism and cell lysis, and provide novel methodology generally applicable to membrane proteins.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

有了这个奖项，化学系的生命过程化学项目正在资助加里教授。Lorigan博士和迈阿密大学化学与生物化学系的一位科学家一起研究了一种被称为pinholin的膜蛋白的结构和动力学特性。新的强大的最先进的技术被用来研究pinholin，这是一个重要的膜结合蛋白，直接参与细胞死亡。病毒感染周期的最后一步是通过裂解细胞死亡，细胞膜的破坏。Lorigan教授计划开发实验程序来表征细胞膜中pinholin的活性和非活性形式的结构和机制。追求允许研究生，本科生和来自代表性不足群体的学生获得尖端电子顺磁共振（EPR）生物物理技术的专业培训。新的生物物理方法的发展是有用的，一般研究各种不同的膜蛋白系统。该研究项目还使迈阿密大学能够开发一个名为DUOS的变革性STEM指导计划，该计划加强了研究生和本科生研究人员之间的协同作用/互动，以便在博士学位教师的监督下合作开展研究项目。授予部门。 Lorigan实验室的一个本科生/研究生团队计划参与这个新项目。该研究项目旨在表征活性和非活性形式的pinholin的结构，拓扑结构，动力学和低聚状态。强大的CW-EPR和脉冲EPR光谱技术被用来研究不同形式的针洞素。在自旋标记之间测量多个距离，以提供关于嵌入膜中的针孔蛋白的重要结构信息。这项研究的信息应提供新的见解pinholin机制和细胞裂解，并提供新的方法，一般适用于membraneprotein.This奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Structural characterization of styrene-maleic acid copolymer-lipid nanoparticles (SMALPs) using EPR spectroscopy

DOI：
10.1016/j.chemphyslip.2019.02.003
发表时间：
2019-05-01
期刊：
CHEMISTRY AND PHYSICS OF LIPIDS
影响因子：
3.4
作者：
Bali, Avnika P.;Sahu, Indra D.;Lorigan, Gary A.
通讯作者：
Lorigan, Gary A.

Solid phase synthesis and spectroscopic characterization of the active and inactive forms of bacteriophage S21 pinholin protein

DOI：
10.1016/j.ab.2018.12.003
发表时间：
2019-02-15
期刊：
ANALYTICAL BIOCHEMISTRY
影响因子：
2.9
作者：
Drew, Daniel L., Jr.;Ahammad, Tanbir;Lorigan, Gary A.
通讯作者：
Lorigan, Gary A.

Investigating the Secondary Structure of Membrane Peptides Utilizing Multiple 2 H-Labeled Hydrophobic Amino Acids via Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy

利用多个 2 H 标记的疏水性氨基酸通过电子自旋回波包络调制 (ESEEM) 光谱研究膜肽的二级结构

DOI：
10.1021/acs.jpcb.7b11890
发表时间：
2018
期刊：
The Journal of Physical Chemistry B
影响因子：
0
作者：
Liu, Lishan;Sahu, Indra D.;Bottorf, Lauren;McCarrick, Robert M.;Lorigan, Gary A.
通讯作者：
Lorigan, Gary A.

Assessing topology and surface orientation of an antimicrobial peptide magainin 2 using mechanically aligned bilayers and electron paramagnetic resonance spectroscopy