ERASynBio: BioMolecular Origami

ERASynBio：生物分子折纸

• 批准号: 1445201
• 负责人: David Baker
• 金额: $ 38.88万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2017-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1445201&HistoricalAwards=false
• 关键词: ERASynBio; BioMolecular; Origami

This project, funded by the Systems and Synthetic Biology Program in MCB and the Biotechnology, Biochemical and Biomass Engineering Program in CBET, is part of a larger ERASynBio funded collaborative. The team of investigators will develop the rules that govern the design of complex nanometer scale structures made from nucleic acids and polypeptides, and use those rules to create new biological molecules that have never been seen before in nature. They will also develop the tools that will enable the assembly of these new materials that could potentially be used to control many aspects of cell function, or create new materials that could be used as sensors or in biomanufacturing.Technical: Biological organisms are capable of producing chemicals, materials and molecular machines that far exceed our engineering capabilities. Underlying these abilities are the unique properties of proteins, exquisitely evolved for function, allowing precise positioning of atoms and chemistries. Designing novel proteins is difficult because of our still incomplete understanding of how proteins fold for a given primary amino-acid sequence. In this project, researchers will apply principles of synthetic biology to define and modularize building blocks that can be combined in rational ways to enable control of 3D positioning in designed macromolecular structure. Members of the consortium have advanced design and engineering principles for polypeptide- and DNA-based nanostructures and developed next-generation gene synthesis to facilitate high-throughput approaches. The team will build on these foundations to engineer bio-macromolecular assemblies with shapes and functions of unprecedented complexity. They will deliver an expanded toolbox of polypeptide building elements; rules, design principles and methods for constructing complex bionanostructures; and routes to nucleic acid/ polypeptide-hybrid platforms for the community of synthetic biology. The project will expand the limits of the designed polypeptide and nucleic acid/protein hybrid providing a platform to facilitate their use in a wide range of biomanufacturing applications.

该项目由MCB的系统和合成生物学计划以及CBET的生物技术，生化和生物量工程计划的资助，是较大的Erasynbio资助协作的一部分。研究人员团队将制定规则，该规则由核酸和多肽制成的复杂纳米尺度结构的设计，并使用这些规则来创建新的生物分子，这些分子在本质上从未见过。 他们还将开发这些工具，这些工具将使这些新材料组装有可能用于控制细胞功能的许多方面，或者创建可以用作传感器或生物制造中的新材料。技术生物：生物生物体能够生产化学物质，材料和分子机器，这些机器远远超过了我们的工程能力。这些能力的基础是蛋白质的独特特性，它为功能而精确地进化，从而可以精确地定位原子和化学物质。由于我们对给定主要氨基酸序列如何折叠的蛋白质如何折叠的蛋白质仍然不完全理解，因此很难设计新型蛋白质。在该项目中，研究人员将应用合成生物学原理来定义和模块化构建块，这些构建块可以合理地结合起来，以控制设计的大分子结构中的3D定位。该财团的成员具有基于多肽和基于DNA的纳米结构的高级设计和工程原理，并开发了下一代基因合成以促进高通量方法。 该团队将以这些基础为基础建立，以设计具有前所未有的复杂性的形状和功能的生物大分子组件。他们将提供扩展的多肽构建元件的工具箱；用于构建复杂bionanostructures的规则，设计原则和方法；以及用于合成生物学社区的核酸/多肽杂交平台的途径。该项目将扩大设计的多肽和核酸/蛋白质混合动力的限制，从而提供一个平台，以促进它们在广泛的生物制造应用中使用。