Development of MAS NMR RF Power and Spinning Speed Controllers and 1H-X Dipolar Recoupling Methods

MAS NMR RF 功率和旋转速度控制器以及 1H-X 偶极重耦合方法的开发

• 批准号: 1608149
• 负责人: Terry Gullion
• 金额: $ 43.4万
• 依托单位: West Virginia University Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1608149&HistoricalAwards=false
• 关键词: Development; MAS; NMR; RF; Power

This project, funded by the Chemical Measurement & Imaging Program of the Chemistry Division, supports Professor Terry Gullion of West Virginia University to develop magnetic resonance methods (analogous to those used in magnetic resonance imaging) to determine the structures of proteins in the solid phase. Proteins are key components in the control of biological processes. Determination of their structures can lead to better understanding of their biochemical functions. For example, interactions of proteins on metallic nanoparticles offer potential therapies for targeting cancer cells. To help communicate this science, Dr. Gullion and his group are developing and presenting chemistry to the public, providing educational opportunities for elementary and middle school students, and developing online tutorials for undergraduate and graduate students.This work focuses on the development of magic-angle spinning nuclear magnetic resonance (MAS NMR) hardware and techniques. The first goal is the development of high-stability MAS NMR hardware necessary for long-term NMR experiments. A controller is being designed to accurately control radio-frequency (rf) power levels as needed for NMR experiments designed to determine molecular structures by dipolar recoupling. A novel method to control the sample spinning speed during MAS NMR experiments is also being developed. This enables NMR experiments that require rf pulse trains to be applied synchronously with the sample rotation. A second goal is to develop MAS NMR 1H-Ag, 1H-Au, 1H-2H dipolar recoupling experiments to determine the structures of peptides chemisorbed on gold and silver nanoparticles and to determine the relative orientation between the adsorbed peptide and the metal surface. To help undergraduate and graduate students that are just beginning to work in solid-state NMR, a series of educational tutorials describing NMR hardware is being produced and posted online.

该项目由化学部的化学测量成像计划资助，支持西弗吉尼亚大学的Terry Gullion教授开发磁共振方法（类似于磁共振成像中使用的方法），以确定固相中蛋白质的结构。 蛋白质是控制生物过程的关键成分。 确定它们的结构可以更好地了解它们的生物化学功能。 例如，蛋白质在金属纳米颗粒上的相互作用为靶向癌细胞提供了潜在的治疗方法。 为了帮助传播这门科学，Gullion博士和他的团队正在开发和向公众展示化学，为小学和中学生提供教育机会，并为本科生和研究生开发在线教程。这项工作的重点是魔角旋转核磁共振（MAS NMR）硬件和技术的开发。 第一个目标是开发长期NMR实验所需的高稳定性MAS NMR硬件。 一个控制器正在设计，以准确地控制所需的核磁共振实验，旨在确定分子结构的偶极再耦合的射频（rf）功率水平。 一种新的方法来控制样品旋转速度在MAS NMR实验也正在开发中。 这使得NMR实验，需要射频脉冲串施加同步样品旋转。 第二个目标是开发MAS NMR 1H-Ag，1H-Au，1H-2 H偶极再耦合实验，以确定化学吸附在金和银纳米颗粒上的肽的结构，并确定吸附的肽和金属表面之间的相对取向。 为了帮助刚开始从事固态NMR工作的本科生和研究生，一系列描述NMR硬件的教育教程正在制作并在线发布。