Enhancing the Performance of High Performance Liquid Chromatography and Mass Spectrometry Using Nanofibers

本项目由美国国家科学基金会化学分部化学测量与成像项目资助。俄亥俄州立大学的Susan Olesik教授正在开发一种分析方法，这种方法能够检测低浓度水平的化合物，与目前的分析能力相比，分析时间缩短了。这项工作可能会影响该国面临的越来越多的挑战，例如需要监测自然水体中的低水平污染物以及需要进行快速临床和法医分析。这项工作的广泛影响包括碳基固定相的发现所带来的潜在社会效益，以及中学生和教师参与这项研究开发的积极科学项目。本项目重点研究液相色谱-质谱联用方法，该方法具有更高的动力学性能、更高的灵敏度和独特的选择性。有四个项目目标。首先，开发了一种碳基开管液相色谱方法。二是研究开发了碳基填充型液相色谱柱。第三，研究了纳米纤维基表面辅助激光解吸电离的机理。第四，开发了与中学研究相兼容的基于纳米纤维的平面色谱法，并将其添加到当地中学的一个新的主动学习和科学推广计划中。

This project is funded by the Chemical Measurement & Imaging Program of the Chemistry Division at the National Science Foundation. Professor Susan Olesik of the Ohio State University is developing analysis methods with the ability to detect compounds at low concentration levels with improved analysis time compared to current capabilities. Increasing challenges in this country such as the need to monitor low levels of pollutants in natural waters and the need for fast clinical and forensic analyses may be impacted by this work. The broader impacts of this work include the potential societal benefits from the discoveries of the carbon based stationary phases as well as, middle school student and teacher engagement in active science projects developed from this research.This project focuses on liquid chromatography-mass spectrometric methods with increased kinetic performance, improved sensitivity, and unique selectivity. There are four project goals. First, a carbon based open tubular liquid chromatographic methods is being developed. Second, a carbon based packed liquid chromatographic column is being studied and developed. Third, the mechanism of nanofiber-based surface assisted laser desorption ionization are studied. Fourth, nanofiber based planar chromatography that is compatible with middle school studies are developed and added to a new active-learning, science outreach program for local middle schools.