3D C-NEMS Based Aptasensors

基于 3D C-NEMS 的适配体传感器

• 批准号: 1611088
• 负责人: Chunlei Wang
• 金额: $ 28.29万
• 依托单位: Florida International University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2021-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1611088&HistoricalAwards=false
• 关键词: 3D; NEMS; Based; Aptasensors

Title: 3D C-NEMS Based AptasensorsBrief description of project Goals: Design, fabricate, evaluate and optimize three dimensional on-chip electrochemical aptasensors based on Carbon-NanoElectroMechanical Systems (C-NEMS).Nontechnical Abstract:The increased amount of proteins such as platelet-derived growth factor (PDGF) can be observed in the early tumorigenesis as well as cancer progression. The PI will focus on developing on-chip capacitive aptasensors employing aptamers as recognition element using unique three dimensional graphene based micropillar arrays, which could deliver high sensitivity, good stability and low detection limits. The miniaturized reconfigurable electrode design is geared to take advantage from the scalable relationship between the interfacial area and overall volume. This project will contribute to the research, education, and diversity goals and strongly support FIUBeyondPossible2020 strategic plan. It will broaden the participation of minority students and women in science and engineering, and foster interdisciplinary interactions. Beyond the impact on the microfabrication research community, this project will have broader impact on cancer diagnosis. The knowledge generated and the key issues identified in this project will impact a broad area of sensor fabrication and development.Technical Abstract:The increased amount of proteins such as platelet-derived growth factor (PDGF) can be observed in the early tumorigenesis as well as cancer progression. In this project, the PI will focus on developing on-chip aptasensors employing aptamers as recognition element based on unique 3D C-MEMS (Carbon-MicroElectroMechanical Systems) and C-NEMS (Carbon-NanoElectroMechanical Systems) platforms. The goal of this project is to design, fabricate, evaluate and optimize the 3D C-NEMS based on-chip electrochemical aptasensors that deliver superior performance (i.e., high sensitivity, good stability, low detection limits).The PI plans to (1) fabricate 3D high-aspect-ratio microelectrode arrays with interdigital fingers by C-MEMS technique; (2) deposit carbon based nanomaterials onto the C-MEMS using electrostatic spray deposition and electrophoretic deposition; (3) functionalize the whole-carbon platform with active chemical groups for efficient and stable immobilization of aptamers; (4) develop and evaluate an electrochemical label-free aptasensor for PDGF protein detection based on characterizing electrochemical impedance and capacitive behaviors. 3D C-NEMS platform will have great advantages and is one of the best solutions to achieve high-sensitivity biosensors. Employing unique C-NEMS technique aptasensors can be fabricated repeatedly with desired dimensions, structures, and material properties. The miniaturized electrode design is geared to take advantage from the scalable relationship between the interfacial area and overall volume. This project will contribute to the research, education, and diversity goals and strongly support FIUBeyondPossible2020 strategic plan. It will broaden the participation of minority students and women in science and engineering. The newly developed technique will be highlighted in the PI's graduate/undergraduate courses. Beyond the impact on the MEMS and NEMS community in academia, the proposed research will have broader impact on cancer diagnosis. The knowledge generated and the key issues identified in this project will impact a broad area of sensor fabrication and development. It is anticipated that the successful implementation of this device in the clinical arena will have significant impact on the early detection, morbidity, and mortality for the large number of patients with cancers, as well as establishing an innovative realm of biomedical technology.

职务名称：基于碳纳米机电系统（C-NEMS）的三维芯片电化学适体传感器的设计、制造、评估和优化。非技术摘要：血小板衍生生长因子（PDGF）等蛋白质的增加可以在早期肿瘤发生和癌症进展中观察到。PI将专注于开发芯片上的电容适体传感器，采用适体作为识别元件，使用独特的三维石墨烯微柱阵列，可以提供高灵敏度，良好的稳定性和低检测限。小型化的可重构电极设计适于利用界面面积和总体积之间的可缩放关系。该项目将有助于研究，教育和多样性目标，并大力支持FIUBeyondPossible 2020战略计划。它将扩大少数民族学生和妇女对科学和工程的参与，并促进跨学科的互动。除了对微加工研究界的影响外，该项目还将对癌症诊断产生更广泛的影响。在这个项目中产生的知识和确定的关键问题将影响传感器的制造和development.Technical摘要广泛的领域：增加的蛋白质，如血小板衍生生长因子（PDGF）可以观察到在早期肿瘤发生以及癌症的进展。在该项目中，PI将专注于开发基于独特的3D C-MEMS（碳微机电系统）和C-NEMS（碳纳米机电系统）平台的芯片适配体传感器，该芯片采用适配体作为识别元件。该项目的目标是设计、制造、评估和优化基于3D C-NEMS的片上电化学适配器传感器，其提供上级性能（即，该研究所计划（1）利用C-MEMS技术制作具有叉指结构的3D高纵横比微电极阵列;（2）利用静电喷雾沉积和电泳沉积将碳基纳米材料沉积到C-MEMS上;（3）利用活性化学基团对全碳平台进行功能化，以有效和稳定地固定适体;（4）利用碳纳米材料的纳米结构和碳纳米材料的纳米结构，以提高碳纳米材料的稳定性。（4）基于电化学阻抗和电容特性，开发和评估用于PDGF蛋白检测的电化学无标记适体传感器。3D C-NEMS平台将具有巨大的优势，是实现高灵敏度生物传感器的最佳解决方案之一。采用独特的C-NEMS技术适配器传感器可以重复制造所需的尺寸，结构和材料特性。微型化电极设计适合利用界面面积和总体积之间的可缩放关系。 该项目将有助于研究，教育和多样性目标，并大力支持FIUBeyondPossible 2020战略计划。它将扩大少数民族学生和妇女对科学和工程的参与。新开发的技术将在PI的研究生/本科课程中突出显示。除了对学术界的MEMS和NEMS社区的影响外，拟议的研究将对癌症诊断产生更广泛的影响。该项目中产生的知识和确定的关键问题将影响传感器制造和开发的广泛领域。预期该装置在临床竞技场的成功实施将对大量癌症患者的早期检测、发病率和死亡率产生重大影响，并建立生物医学技术的创新领域。