3D C-NEMS Based Aptasensors

项目目标：设计、制造、评估和优化基于碳纳米机电系统（C-NEMS）的三维片上电化学aptassensor。摘要：血小板衍生生长因子（platelet-derived growth factor， PDGF）等蛋白在肿瘤发生早期和肿瘤进展过程中均有升高。PI将专注于开发片上电容式适配体传感器，该传感器采用独特的三维石墨烯微柱阵列，采用适配体作为识别元件，可以提供高灵敏度，良好的稳定性和低检测限。小型化的可重构电极设计旨在利用界面面积和整体体积之间的可扩展关系。该项目将有助于实现研究、教育和多样性目标，并有力地支持复旦大学超越可能2020战略计划。它将扩大少数民族学生和妇女在科学和工程领域的参与，并促进跨学科的互动。除了对微加工研究界的影响外，该项目将对癌症诊断产生更广泛的影响。在这个项目中产生的知识和确定的关键问题将影响传感器制造和开发的广泛领域。技术摘要：血小板衍生生长因子（platelet-derived growth factor， PDGF）等蛋白在肿瘤发生早期和肿瘤进展过程中均有升高。在该项目中，PI将专注于开发基于独特的3D C-MEMS（碳-微机电系统）和C-NEMS（碳纳米机电系统）平台的片上适配体传感器，该传感器采用适配体作为识别元件。该项目的目标是设计，制造，评估和优化基于3D C-NEMS的片上电化学配体传感器，该传感器具有优异的性能（即高灵敏度，良好的稳定性，低检测限）。PI计划(1)利用C-MEMS技术制造具有手指间的三维高纵横比微电极阵列；(2)采用静电喷涂和电泳沉积方法在C-MEMS上沉积碳基纳米材料；(3)利用活性化学基团对全碳平台进行功能化，实现适配体的高效稳定固定化；(4)基于电化学阻抗和电容行为特征，开发并评价一种用于PDGF蛋白检测的电化学无标记适配体传感器。3D C-NEMS平台将具有很大的优势，是实现高灵敏度生物传感器的最佳解决方案之一。采用独特的C-NEMS技术，可以重复制造具有所需尺寸，结构和材料特性的传感器。小型化的电极设计是为了利用界面面积和整体体积之间的可扩展关系。该项目将有助于实现研究、教育和多样性目标，并有力地支持复旦大学超越可能2020战略计划。它将扩大少数民族学生和妇女在科学和工程领域的参与。新开发的技术将在PI的研究生/本科课程中得到重点介绍。除了对学术界的MEMS和NEMS社区产生影响外，拟议的研究将对癌症诊断产生更广泛的影响。在这个项目中产生的知识和确定的关键问题将影响传感器制造和开发的广泛领域。预计该装置在临床领域的成功实施将对大量癌症患者的早期发现、发病率和死亡率产生重大影响，并建立一个创新的生物医学技术领域。

Title: 3D C-NEMS Based AptasensorsBrief description of project Goals: Design, fabricate, evaluate and optimize three dimensional on-chip electrochemical aptasensors based on Carbon-NanoElectroMechanical Systems (C-NEMS).Nontechnical Abstract:The increased amount of proteins such as platelet-derived growth factor (PDGF) can be observed in the early tumorigenesis as well as cancer progression. The PI will focus on developing on-chip capacitive aptasensors employing aptamers as recognition element using unique three dimensional graphene based micropillar arrays, which could deliver high sensitivity, good stability and low detection limits. The miniaturized reconfigurable electrode design is geared to take advantage from the scalable relationship between the interfacial area and overall volume. This project will contribute to the research, education, and diversity goals and strongly support FIUBeyondPossible2020 strategic plan. It will broaden the participation of minority students and women in science and engineering, and foster interdisciplinary interactions. Beyond the impact on the microfabrication research community, this project will have broader impact on cancer diagnosis. The knowledge generated and the key issues identified in this project will impact a broad area of sensor fabrication and development.Technical Abstract:The increased amount of proteins such as platelet-derived growth factor (PDGF) can be observed in the early tumorigenesis as well as cancer progression. In this project, the PI will focus on developing on-chip aptasensors employing aptamers as recognition element based on unique 3D C-MEMS (Carbon-MicroElectroMechanical Systems) and C-NEMS (Carbon-NanoElectroMechanical Systems) platforms. The goal of this project is to design, fabricate, evaluate and optimize the 3D C-NEMS based on-chip electrochemical aptasensors that deliver superior performance (i.e., high sensitivity, good stability, low detection limits).The PI plans to (1) fabricate 3D high-aspect-ratio microelectrode arrays with interdigital fingers by C-MEMS technique; (2) deposit carbon based nanomaterials onto the C-MEMS using electrostatic spray deposition and electrophoretic deposition; (3) functionalize the whole-carbon platform with active chemical groups for efficient and stable immobilization of aptamers; (4) develop and evaluate an electrochemical label-free aptasensor for PDGF protein detection based on characterizing electrochemical impedance and capacitive behaviors. 3D C-NEMS platform will have great advantages and is one of the best solutions to achieve high-sensitivity biosensors. Employing unique C-NEMS technique aptasensors can be fabricated repeatedly with desired dimensions, structures, and material properties. The miniaturized electrode design is geared to take advantage from the scalable relationship between the interfacial area and overall volume. This project will contribute to the research, education, and diversity goals and strongly support FIUBeyondPossible2020 strategic plan. It will broaden the participation of minority students and women in science and engineering. The newly developed technique will be highlighted in the PI's graduate/undergraduate courses. Beyond the impact on the MEMS and NEMS community in academia, the proposed research will have broader impact on cancer diagnosis. The knowledge generated and the key issues identified in this project will impact a broad area of sensor fabrication and development. It is anticipated that the successful implementation of this device in the clinical arena will have significant impact on the early detection, morbidity, and mortality for the large number of patients with cancers, as well as establishing an innovative realm of biomedical technology.