MRI: Acquisition of a Liquid Chromatograph Mass Spectrometer for Research and Education in the Rural Western Illinois Region

MRI：采购一台液相色谱质谱仪，用于伊利诺伊州西部农村地区的研究和教育

• 批准号: 1827209
• 负责人: Liguo Song
• 金额: $ 29.97万
• 依托单位: Western Illinois University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1827209&HistoricalAwards=false
• 关键词: MRI; Acquisition; Liquid; Chromatograph; Mass

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Professor Liguo Song from Western Illinois University (WIU) and colleagues Jenq-Kuen Huang (WIU), Meshack Afitlhile (WIU), Audra Sostarecz from Monmouth College, and Caitlin Deskins from Quincy University have acquired a high-resolution quadrupole, time-of-flight, liquid chromatograph mass spectrometer (Q-TOF-LCMS). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex samples. In a typical experiment, the components flow into a mass spectrometer where they are ionized into ions and then the masses of the ions are measured. This highly sensitive technique allows the structure of molecules in complex mixtures to be studied. An instrument with a liquid chromatograph can separate mixtures of compounds before they reach the mass spectrometer. In the time-of-flight method, the mass-to-charge ratio of an ion is determined by the way of a time measurement in which ions are accelerated by an electric field of known strength. This acceleration results in an ion having the same kinetic energy as any other ion that has the same charge. The acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse groups of students in research and research training using this modern analytical technique. It also provides training opportunities to many undergraduate students including those from underrepresented groups and will serve as a regional resource serving neighboring universities, agricultural associations and in institutions that need forensics analyses.The proposal is aimed at enhancing research and education at all levels. It especially impacts trace level analysis of drugs of abuse in hair, investigation of the self-heating and spontaneous ignition process of vegetable oils, and investigations of the structure-function of antibacterial molecules with Langmuir monolayer films. The mass spectrometer aids researchers studying metabolic engineering of secondary alcohol dehydrogenase from the bacterium nocardia cholesterolicum NRRL 5767 via CRISP/Cas9 genome editing and those investigating the slow pH-dependent equilibrium of protease inhibitor leupeptin. The instrument is also essential for the analyses of volatile compounds such as those in breads.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要研究仪器和化学研究仪器计划支持。来自西伊利诺伊大学（WIU）的Liguo Song教授和同事Jenq-Kuen Huang（WIU），Meshack Afitlhile（WIU），来自蒙茅斯学院的Audra Sostarecz和来自昆西大学的Caitlin Deskins获得了高分辨率四极杆飞行时间液相色谱质谱仪（Q-TOF-LCMS）。一般来说，质谱（MS）是用于识别和表征复杂样品中嵌入的少量化学物质的关键分析方法之一。在一个典型的实验中，成分流入质谱仪，在那里它们被电离成离子，然后测量离子的质量。这种高灵敏度的技术可以研究复杂混合物中的分子结构。带有液相色谱仪的仪器可以在化合物到达质谱仪之前将其分离。在飞行时间方法中，离子的质荷比通过时间测量的方式来确定，其中离子被已知强度的电场加速。这种加速导致离子具有与具有相同电荷的任何其他离子相同的动能。此次收购加强了大学和地区的研究基础设施。该工具通过利用这一现代分析技术使不同群体的学生参与研究和研究培训，扩大了参与。它还为许多本科生提供培训机会，包括来自代表性不足群体的本科生，并将作为区域资源，为邻近大学、农业协会和需要法医分析的机构提供服务。该提案旨在加强各级研究和教育。它特别影响头发中滥用药物的痕量分析，植物油自加热和自燃过程的研究，以及用Langmuir单层膜研究抗菌分子的结构-功能。质谱仪帮助研究人员通过CRISP/Cas9基因组编辑研究来自细菌Nocardia cholesterolicum NRRL 5767的仲醇脱氢酶的代谢工程，以及研究蛋白酶抑制剂亮抑酶肽的缓慢pH依赖性平衡的研究人员。该仪器对于分析面包等挥发性化合物也是必不可少的。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Quantification of meclofenamic acid in equine plasma by ultra-high-performance liquid chromatography electrospray ionization tandem mass spectrometry (UHPLC-ESI/MS/MS)

通过超高效液相色谱电喷雾电离串联质谱 (UHPLC-ESI/MS/MS) 定量马血浆中的甲氯芬那酸

• 发表时间: 2021
• 期刊: 113rd Illinois State Academy of Science Annual Meeting
• 作者: Chao, M.

Knockout of a secondary alcohol dehydrogenase gene in Nocardia cholesterolicum NRRL 5767 by CRISPR-Cas9 technology

通过 CRISPR-Cas9 技术敲除胆固醇诺卡氏菌 NRRL 5767 中的二级醇脱氢酶基因

• 发表时间: 2019
• 期刊: 2019 ACS Midwest Regional Meeting
• 作者: Huang, J.K.;Samassekou, K.;Seiver, J.;McClenahan, S.;Holt, S.;Wen, L.
• 通讯作者: Wen, L.

Quantification of phenylbutazone in equine plasma for doping control in horse racing using strong anion exchange solid phase extraction followed by liquid chromatography with UV detection

使用强阴离子交换固相萃取和紫外检测液相色谱对马血浆中保泰松进行定量，用于赛马兴奋剂控制

• 发表时间: 2019
• 期刊: 111th Illinois State Academy of Science Annual Meeting
• 作者: Lin, F.;Heiser, N.;Song, L.
• 通讯作者: Song, L.

Quantification of phenylbutazone in equine plasma by ultra-high-performance liquid chromatography electrospray ionization tandem mass spectrometry (UHPLC-ESI/MS/MS)

通过超高效液相色谱电喷雾电离串联质谱 (UHPLC-ESI/MS/MS) 定量马血浆中的保泰松

• 发表时间: 2021
• 期刊: 113rd Illinois State Academy of Science Annual Meeting
• 作者: Chao, M.

High-Throughput and Simultaneous Analysis of 12 Cannabinoids in Hemp Oil Using Liquid Chromatography With Ultraviolet (LC-UV) Detection

使用具有紫外 (LC-UV) 检测功能的液相色谱法对大麻油中的 12 种大麻素进行高通量同步分析

• 发表时间: 2020
• 期刊: 2020 American Academy of Forensic Sciences Annual Scientific Meeting
• 作者: Song, L.;Pathipaka, S.B.;Leese, J.D.;Chao, M.;Collins, T.;Westein, J.P.
• 通讯作者: Westein, J.P.